{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:23:30Z","timestamp":1760059410094,"version":"build-2065373602"},"reference-count":26,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Alveolar echinococcosis (AE), caused by Echinococcus multilocularis (E. multilocularis), is a severe parasitic zoonosis that is potentially fatal for humans. The parasite is primarily transmitted by wildlife, with red foxes acting as definitive hosts and rodents as intermediate hosts, while humans can become accidental but dead-end hosts. The aim of this study is to use EmsB typing on E. multilocularis isolates from human AE cases and local animals such as foxes and rodents. In this study, retrospective EmsB typing was performed on 39 samples, including 11 tissue samples from 10 patients, 18 fecal swabs from foxes, and 10 tissue samples from rodents. A dendrogram was created to determine the EmsB profiles present. The results showed that all the rodent samples were associated with the EmsB P1 profile (10\/10), while the human and fox samples shared the EmsB profile P1 (5\/11 humans and 8\/18 foxes), a profile near P4 (2\/11 humans and 3 foxes), and a profile near P8 (1\/11 humans and 1\/18 foxes). The study demonstrates that the same EmsB profiles circulate among humans and animals, confirming that wildlife reservoirs play a key role in transmission.<\/jats:p>","DOI":"10.3390\/pathogens14060584","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T06:42:48Z","timestamp":1749710568000},"page":"584","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["EmsB Microsatellite Analysis of Echinococcus multilocularis Specimens Isolated from Belgian Patients with Alveolar Echinococcosis and from Animal Hosts"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3825-7560","authenticated-orcid":false,"given":"Sabrina","family":"Egrek","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology\u2014Belgian National Reference Laboratory for Echinococcosis, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Liege, 4000 Liege, Belgium"},{"name":"EchinoLi\u00e8ge, University Hospital of Liege (CHU-ULiege), Avenue de l\u2019H\u00f4pital, 1, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2054-1619","authenticated-orcid":false,"given":"Jenny","family":"Knapp","sequence":"additional","affiliation":[{"name":"Laboratory Chrono-Environment UMR CNRS 6249, University Marie & Louis Pasteur, 25030 Besan\u00e7on, France"},{"name":"Department of Parasitology-Mycology, National Reference Center for Echinococcoses, University Hospital of Besan\u00e7on, 25030 Besan\u00e7on, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3922-324X","authenticated-orcid":false,"given":"Rosalie","family":"Sacheli","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology\u2014Belgian National Reference Laboratory for Echinococcosis, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Liege, 4000 Liege, Belgium"},{"name":"EchinoLi\u00e8ge, University Hospital of Liege (CHU-ULiege), Avenue de l\u2019H\u00f4pital, 1, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3317-377X","authenticated-orcid":false,"given":"Khalid","family":"El Moussaoui","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology\u2014Belgian National Reference Laboratory for Echinococcosis, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Liege, 4000 Liege, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"L\u00e9onard","sequence":"additional","affiliation":[{"name":"EchinoLi\u00e8ge, University Hospital of Liege (CHU-ULiege), Avenue de l\u2019H\u00f4pital, 1, 4000 Li\u00e8ge, Belgium"},{"name":"Department of Infectious and Tropical Diseases, University Hospital of Liege, 4000 Liege, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0792-3346","authenticated-orcid":false,"given":"Eva","family":"Larranaga Lapique","sequence":"additional","affiliation":[{"name":"Department Clinic of Infectious Disease, University Hospital of Brussels, 1070 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5939-3409","authenticated-orcid":false,"given":"Laurence","family":"Millon","sequence":"additional","affiliation":[{"name":"Laboratory Chrono-Environment UMR CNRS 6249, University Marie & Louis Pasteur, 25030 Besan\u00e7on, France"},{"name":"Department of Parasitology-Mycology, National Reference Center for Echinococcoses, University Hospital of Besan\u00e7on, 25030 Besan\u00e7on, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Engelskirchen","sequence":"additional","affiliation":[{"name":"Fundamental and Applied Research for Animals and Health (FARAH), Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9436-6673","authenticated-orcid":false,"given":"Olivier","family":"Detry","sequence":"additional","affiliation":[{"name":"EchinoLi\u00e8ge, University Hospital of Liege (CHU-ULiege), Avenue de l\u2019H\u00f4pital, 1, 4000 Li\u00e8ge, Belgium"},{"name":"Department of Abdominal Surgery and Transplantation, University Hospital of Liege, 4000 Liege, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annick","family":"Linden","sequence":"additional","affiliation":[{"name":"EchinoLi\u00e8ge, University Hospital of Liege (CHU-ULiege), Avenue de l\u2019H\u00f4pital, 1, 4000 Li\u00e8ge, Belgium"},{"name":"Fundamental and Applied Research for Animals and Health (FARAH), Faculty of Veterinary Medicine, University of Liege, 4000 Liege, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9025-6493","authenticated-orcid":false,"given":"Marie-Pierre","family":"Hayette","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology\u2014Belgian National Reference Laboratory for Echinococcosis, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Liege, 4000 Liege, Belgium"},{"name":"EchinoLi\u00e8ge, University Hospital of Liege (CHU-ULiege), Avenue de l\u2019H\u00f4pital, 1, 4000 Li\u00e8ge, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1093\/clinids\/15.2.234","article-title":"Parasiticidal Effect of Chemotherapy in Alveolar Hydatid Disease: Review of Experience with Mebendazole and Albendazole in Alaskan 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