{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:13:58Z","timestamp":1792872838824,"version":"4.5.0"},"reference-count":82,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T00:00:00Z","timestamp":1750377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The wild boar (Sus scrofa) has experienced significant population growth as well as geographic expansion across Europe over the past 15 years, leading to increased concerns regarding its role in the transmission of zoonotic pathogens. Among these, Babesia spp. and Anaplasma spp. are of particular importance due to their impact on both wildlife and domestic animals. This study systematically reviews the prevalence and distribution of Babesia and Anaplasma spp. in wild boars and associated tick vectors across multiple European countries, synthesizing data from literature published between 2010 and 2024. A comprehensive search of Scopus, Google Scholar, and PubMed databases was conducted using predefined keywords related to babesiosis, anaplasmosis, wild boars, Europe, and tick-borne diseases. A total of 281 studies were initially retrieved, of which 19 met the inclusion criteria following relevance assessment. Data extraction focused on pathogen identification, diagnostic methods, sample type, host species, and prevalence rates. Molecular detection methods, primarily PCR and sequencing, were the most used diagnostic tools. Results indicate substantial regional variations in the prevalence of Babesia and Anaplasma spp. A. phagocytophilum was detected in wild boar populations across multiple countries, with the highest prevalence rates observed in Slovakia (28.2%) and Poland (20.34%). Conversely, lower prevalence rates were recorded in France (2%) and Portugal (3.1%). Babesia spp. showed higher prevalence rates in Italy (6.2%), while its detection in other regions such as Romania and Spain was minimal or absent. Notably, spleen and multi-organ samples (spleen\/liver\/kidney) exhibited higher positivity rates compared to blood samples, suggesting an organotropic localization of these pathogens. The findings underscore the role of wild boars as reservoirs for tick-borne pathogens and highlight their potential to contribute to the epidemiological cycle of these infections. The increasing distribution of wild boars, coupled with climate-driven shifts in tick populations, may further facilitate pathogen transmission. Future studies should focus on integrating molecular, serological, and ecological approaches to improve surveillance and risk assessment. Standardized methodologies across different regions will be essential in enhancing comparative epidemiological insights and informing targeted disease management strategies.<\/jats:p>","DOI":"10.3390\/pathogens14070612","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T05:17:42Z","timestamp":1750396662000},"page":"612","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A Systematic Review on the Occurrence of Babesia spp. and Anaplasma spp. in Ticks and Wild Boar from Europe\u2014A 15-Year Retrospective Study"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4207-473X","authenticated-orcid":false,"given":"Ioan Cristian","family":"Dreghiciu","sequence":"first","affiliation":[{"name":"Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3659-1517","authenticated-orcid":false,"given":"Diana","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiana","family":"Florea","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ion","family":"Oprescu","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7058-4430","authenticated-orcid":false,"given":"Simona","family":"Dumitru","sequence":"additional","affiliation":[{"name":"Veterinary and Food Safety Department 4, Surorile Martir Caceu, 300585 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3004-463X","authenticated-orcid":false,"given":"Mirela","family":"Imre","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vlad","family":"Iorgoni","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1888-2597","authenticated-orcid":false,"given":"Anamaria","family":"Plesko","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorin","family":"Morariu","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5484-4685","authenticated-orcid":false,"given":"Marius Stelian","family":"Ilie","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Disease, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d of Timisoara, 119, Calea Aradului, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.7589\/0090-3558-46.3.803","article-title":"Experimental Infection in Lambs with a Red Deer (Cervus elaphus) Isolate of Anaplasma phagocytophilum","volume":"46","author":"Stuen","year":"2010","journal-title":"J. Wildl. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1111\/mam.12015","article-title":"A review of wild boar Sus scrofa diet and factors affecting food selection in native and introduced ranges","volume":"44","author":"Ballari","year":"2014","journal-title":"Mammal Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"135","DOI":"10.7325\/Galemys.2004.NE.A11","article-title":"The environmental impact of wild boar","volume":"16","author":"Massei","year":"2004","journal-title":"Galemys"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Pittiglio, C., Khomenko, S., and Beltran-Alcrudo, D. (2018). Wild boar mapping using population-density statistics: From polygons to high resolution raster maps. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0193295"},{"key":"ref_5","unstructured":"Apollonio, M., Andersen, R., and Putman, R. (2010). European Ungulates and Their Management in the 21st Century, Cambridge University Press."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1002\/ps.3965","article-title":"Wild boar populations up, numbers of hunters down? A review of trends and implications for Europe","volume":"71","author":"Massei","year":"2015","journal-title":"Pest Manag. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Lewis, J.S., Farnsworth, M.L., Burdett, C.L., Theobald, D.M., Gray, M., and Miller, R.S. (2017). Biotic and abiotic factors predicting the global distribution and population density of an invasive large mammal. Sci. Rep., 7.","DOI":"10.1038\/srep44152"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1214","DOI":"10.1111\/1365-2656.12721","article-title":"Niche conservatism and the invasive potential of the wild boar","volume":"86","author":"Sales","year":"2017","journal-title":"J. Anim. Ecol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"McClure, M.L., Burdett, C.L., Farnsworth, M.L., Lutman, M.W., Theobald, D.M., Riggs, P.D., Grear, D.A., and Miller, R.S. (2015). Modeling and mapping the probability of occurrence of invasive wild pigs across the contiguous United States. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0133771"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.1111\/1365-2664.12866","article-title":"Interpreting and predicting the spread of invasive wild pigs","volume":"54","author":"Snow","year":"2017","journal-title":"J. Appl. Ecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.agee.2016.02.016","article-title":"Invading or recolonizing? Patterns and drivers of wild boar population expansion into Belgian agroecosystems","volume":"222","author":"Morelle","year":"2016","journal-title":"Agric. Ecosyst. Environ."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1016\/j.scitotenv.2019.06.157","article-title":"Where winter rules: Modeling wild boar distribution in its north-eastern range","volume":"687","author":"Markov","year":"2019","journal-title":"Sci. Total Environ."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1111\/j.1365-2664.2005.01094.x","article-title":"Population dynamics in wild boar Sus scrofa: Ecology, elasticity of growth rate and implications for the management of pulsed resource consumers","volume":"42","author":"Bieber","year":"2005","journal-title":"J. Appl. Ecol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/BF00317805","article-title":"Wolf predation and snow cover as mortality factors in the ungulate community of the Bia\u0142owie\u017ca National Park, Poland","volume":"90","author":"Okarma","year":"1992","journal-title":"Oecologia"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1007\/s10344-024-01850-y","article-title":"Hunting bag statistics of wild mammals in Portugal (1989\u20132022): On the need to improve data report and compilation","volume":"70","author":"Carvalho","year":"2024","journal-title":"Eur. J. Wildl. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"110041","DOI":"10.1016\/j.ecolmodel.2022.110041","article-title":"Revisiting wild boar spatial models based on hunting yields to assess their predictive performance on interpolation and extrapolation areas","volume":"471","author":"Vicente","year":"2022","journal-title":"Ecol. Model."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bergqvist, G., Kindberg, J., and Elmhagen, B. (2024). From virtually extinct to superabundant in 35 years: Establishment, population growth and shifts in management focus of the Swedish wild boar (Sus scrofa) population. BMC Zool., 9.","DOI":"10.1186\/s40850-024-00202-2"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"171","DOI":"10.15407\/TU2415","article-title":"Population density of the wild boar (Sus scrofa) in south-western Poland in 1981\u20132020","volume":"24","author":"Kopij","year":"2022","journal-title":"Theriologia Ukrainica"},{"key":"ref_19","first-page":"1294","article-title":"Babesiosis in domestic animals in China: Epidemiology and diagnosis","volume":"67","author":"Yin","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_20","first-page":"1655","article-title":"A new species of Babesia infective to domestic pigs","volume":"77","author":"Hikosaka","year":"2015","journal-title":"J. Vet. Med. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s13071-014-0613-4","article-title":"Diagnosis and incidence risk of clinical canine monocytic ehrlichiosis under field conditions in Southern Europe","volume":"8","author":"Lebert","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_22","unstructured":"Coetzer, J.A.W., Thomson, G.E., Maclachlan, N.J., and Penrith, M.L. (2019). Porcine babesiosis. Infectious Diseases of Livestock, Anipedia."},{"key":"ref_23","first-page":"1","article-title":"Molecular detection and characterization of Anaplasma species in wild boars (Sus scrofa) from North-Eastern Romania","volume":"11","author":"Dumitrache","year":"2006","journal-title":"Parasit Vectors"},{"key":"ref_24","first-page":"1","article-title":"Anaplasma phagocytophilum and Anaplasma marginale: Comparison of nucleotide sequences for phylogenetic studies","volume":"18","author":"Galon","year":"2018","journal-title":"BMC Microbiol."},{"key":"ref_25","first-page":"98","article-title":"Molecular Detection of Anaplasma phagocytophilum in Wild Boars (Sus scrofa) from Romania","volume":"228","author":"Mihalca","year":"2016","journal-title":"Vet. Parasitol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.nmni.2018.08.017","article-title":"Prevalence of Anaplasma phagocytophilum and Coxiella burnetii in Ixodes ricinus Ticks in Switzerland: An Underestimated Epidemiologic Risk","volume":"27","author":"Pilloux","year":"2019","journal-title":"New Microbes New Infect."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1128\/JCM.02051-10","article-title":"Distinct Host Species Correlate with Anaplasma phagocytophilum ankA Gene Clusters","volume":"49","author":"Scharf","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1038\/nrmicro2318","article-title":"Anaplasma phagocytophilum and Ehrlichia chaffeensis: Subversive Manipulators of Host Cells","volume":"8","author":"Rikihisa","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1099\/00222615-47-3-235","article-title":"Ultrastructural Differentiation of the Genogroups in the Genus Ehrlichia","volume":"47","author":"Popov","year":"1998","journal-title":"J. Med. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4696","DOI":"10.1128\/IAI.05658-11","article-title":"Proteomic Analysis of Anaplasma phagocytophilum During Infection of Human Myeloid Cells Identifies a Protein that is Pronouncedly Upregulated on the Infectious Dense-Cored Cell","volume":"79","author":"Troese","year":"2011","journal-title":"Infect. Immun."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.medmal.2019.01.007","article-title":"Ticks and tick-borne diseases","volume":"49","author":"Boulanger","year":"2019","journal-title":"Med. Mal Infect."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1586\/14760584.2015.1076339","article-title":"Tick vaccines: Current status and future directions","volume":"14","author":"Contreras","year":"2015","journal-title":"Expert Rev. Vaccines"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Estrada-Pe\u00f1a, A., Gray, J.S., Kahl, O., Lane, R.S., and Nijhof, A.M. (2013). Research on the ecology of ticks and tick-borne pathogens\u2014methodological principles and caveats. Front. Cell Infect Microbiol., 3.","DOI":"10.3389\/fcimb.2013.00029"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-6-1","article-title":"Driving forces for changes in geographical distribution of Ixodes ricinus ticks in Europe","volume":"6","author":"Medlock","year":"2013","journal-title":"Parasit Vectors."},{"key":"ref_35","first-page":"n71","article-title":"The PRISMA 2020 statement: An updated guideline for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ Clin. Res. Ed."},{"key":"ref_36","unstructured":"(2025, March 16). MapChart. Available online: https:\/\/www.mapchart.net\/europe.html."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Defaye, B., Moutailler, S., Pietri, C., Galon, C., Grech-Angelini, S., Pasqualini, V., and Quilichini, Y. (2021). Molecular Detection of Zoonotic and Non-Zoonotic Pathogens from Wild Boars and Their Ticks in the Corsican Wetlands. Pathogens, 10.","DOI":"10.3390\/pathogens10121643"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"101936","DOI":"10.1016\/j.ttbdis.2022.101936","article-title":"Diversity of Tick Species and Associated Pathogens on Peri-Urban Wild Boars\u2014First Report of the Zoonotic Babesia cf. crassa from Hungary","volume":"13","author":"Hornok","year":"2022","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1186\/1756-3305-7-70","article-title":"Piroplasmosis in Wildlife: Babesia and Theileria Affecting Free-Ranging Ungulates and Carnivores in the Italian Alps","volume":"7","author":"Zanet","year":"2014","journal-title":"Parasit. Vectors"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1089\/vbz.2014.1648","article-title":"Molecular Epizootiology and Diagnosis of Porcine Babesiosis in Sardinia, Italy","volume":"14","author":"Zobba","year":"2014","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sgroi, G., D\u2019Alessio, N., Auriemma, C., Salant, H., Gallo, A., Riccardi, M.G., Alfano, F., Rea, S., Scarcelli, S., and Ottaviano, M. (2023). First Molecular Detection of Babesia vulpes and Babesia capreoli in Wild Boars from Southern Italy. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1201476"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Matei, I.A., Kalm\u00e1r, Z., Balea, A., Mihaiu, M., S\u00e1ndor, A.D., Cocian, A., Cr\u0103ciun, S., Bouari, C., Briciu, V.T., and Fi\u021b, N. (2023). The Role of Wild Boars in the Circulation of Tick-Borne Pathogens: The First Evidence of Rickettsia monacensis Presence. Animals, 13.","DOI":"10.3390\/ani13111743"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1111\/tbed.14065","article-title":"Prevalence of Selected Tick-Borne Pathogens in Wild Ungulates and Ticks in Southern Spain","volume":"69","author":"Adaszek","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1186\/s13071-021-04860-w","article-title":"Wild ungulate species differ in their contribution to the transmission of Ixodes ricinus-borne pathogens","volume":"14","author":"Fabri","year":"2021","journal-title":"Parasit Vectors"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.ttbdis.2015.07.016","article-title":"Babesia species in questing Ixodes ricinus, Sweden","volume":"7","author":"Karlsson","year":"2016","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Nahayo, A., Bardiau, M., Volpe, R., Pirson, J., Paternostre, J., Fett, T., and Linden, A. (2014). Molecular Evidence of Anaplasma phagocytophilum in Wild Boar (Sus scrofa) in Belgium. BMC Vet. Res., 10.","DOI":"10.1186\/1746-6148-10-80"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"101558","DOI":"10.1016\/j.ttbdis.2020.101558","article-title":"Wild Boar as a Potential Reservoir of Zoonotic Tick-Borne Pathogens","volume":"12","author":"Lesiczka","year":"2021","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1111\/tbed.12508","article-title":"Identification of a Potential Marker of Anaplasma phagocytophilum Associated with Cattle Abortion","volume":"64","author":"Dugat","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_49","first-page":"745","article-title":"Tick-Borne Pathogens in Ticks (Acari: Ixodidae) Collected from Various Domestic and Wild Hosts in Corsica (France), a Mediterranean Island Environment","volume":"67","author":"Stachurski","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1089\/vbz.2013.1495","article-title":"Molecular Investigation for Bacterial and Protozoan Tick-Borne Pathogens in Wild Boars (Sus scrofa) from Southern Germany","volume":"14","author":"Silaghi","year":"2014","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"141","DOI":"10.12980\/apjtd.7.2017D6-412","article-title":"Zoonotic Tick-Borne Bacteria among Wild Boars (Sus scrofa) in Central Italy","volume":"7","author":"Ebani","year":"2017","journal-title":"Asian Pac. J. Trop. Med. Press"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Myczka, A.W., Steiner-Bogdaszewska, \u017b., Filip-Hutsch, K., Olo\u015b, G., Czopowicz, M., and Laskowski, Z. (2021). Detection of Anaplasma phagocytophilum in Wild and Farmed Cervids in Poland. Pathogens, 10.","DOI":"10.3390\/pathogens10091190"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Michalski, M.M., Kubiak, K., Szczotko, M., and Dmitryjuk, M. (2021). Tick-Borne Pathogens in Ticks Collected from Wild Ungulates in North-Eastern Poland. Pathogens, 10.","DOI":"10.3390\/pathogens10050587"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1186\/s13071-016-1535-0","article-title":"Molecular Detection of Tick-Borne Bacteria and Protozoa in Cervids and Wild Boars from Portugal","volume":"9","author":"Pereira","year":"2016","journal-title":"Parasit. Vectors"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1017\/S0950268813000812","article-title":"Prevalence of Anaplasma phagocytophilum Infection in European Wild Boar (Sus scrofa) Populations from Transylvania, Romania","volume":"142","author":"Kiss","year":"2014","journal-title":"Epidemiol. Infect."},{"key":"ref_56","first-page":"59","article-title":"Molecular Detection of Anaplasma phagocytophilum in Wild Boar (Sus scrofa) from Hunedoara and Timi\u0219 Counties\u2014Preliminary Study","volume":"33","author":"Dreghiciu","year":"2023","journal-title":"Rev. Rom. Med. Vet."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1186\/s13071-018-3068-1","article-title":"Diverse Tick-Borne Microorganisms Identified in Free-Living Ungulates in Slovakia","volume":"11","author":"Caban","year":"2018","journal-title":"Parasit. Vectors"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.1128\/JCM.42.5.2285-2286.2004","article-title":"Molecular Evidence of Anaplasma phagocytophilum in Ixodes ricinus Ticks and Wild Animals in Austria","volume":"42","author":"Polin","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1089\/vbz.2009.0214","article-title":"Anaplasma spp. in Wild Mammals and Ixodes ricinus from the North of Spain","volume":"11","author":"Portillo","year":"2011","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S0304-4017(02)00417-X","article-title":"Ixodes hexagonus is the Main Candidate as Vector of Theileria annae in Northwest Spain","volume":"112","author":"Camacho","year":"2003","journal-title":"Vet. Parasitol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1186\/1756-3305-6-190","article-title":"Spatial Disaggregation of Tick Occurrence and Ecology at a Local Scale as a Preliminary Step for Spatial Surveillance of Tick-Borne Diseases: General Framework and Health Implications in Belgium","volume":"6","author":"Obsomer","year":"2013","journal-title":"Parasit. Vectors"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Estrada-Pe\u00f1a, A., Mihalca, A.D., and Petney, T.N. (2017). Ticks of Europe and North Africa: A Guide to Species Identification, Springer Nature.","DOI":"10.1007\/978-3-319-63760-0"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1186\/s13071-021-05019-3","article-title":"The Specificity of Babesia-Tick Vector Interactions: Recent Advances and Pitfalls in Molecular and Field Studies","volume":"14","author":"Bajer","year":"2021","journal-title":"Parasit. Vectors"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1186\/1756-3305-5-181","article-title":"Gene Expression Profile Suggests that Pigs (Sus scrofa) are Susceptible to Anaplasma phagocytophilum but Control Infection","volume":"5","author":"Galindo","year":"2012","journal-title":"Parasites Vectors"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Zhang, L., Wang, G., Liu, Q., Chen, C., Li, J., Long, B., Yu, H., Zhang, Z., He, J., and Qu, Z. (2013). Molecular analysis of Anaplasma phagocytophilum isolated from patients with febrile diseases of unknown etiology in China. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057155"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1046\/j.1365-2915.1998.00133.x","article-title":"Granulocytic Ehrlichia infection in ixodid ticks and mammals in woodlands and uplands of the U.K","volume":"12","author":"Ogden","year":"1998","journal-title":"Med. Vet. Entomol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1128\/JCM.40.3.892-897.2002","article-title":"PCR detection and serological evidence of granulocytic ehrlichial infection in roe deer (Capreolus capreolus) and chamois (Rupicapra rupicapra)","volume":"40","author":"Liz","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1128\/AEM.03300-12","article-title":"Anaplasma phagocytophilum in questing Ixodes ricinus Ticks: Comparison of prevalences and partial 16S rRNA gene variants in urban, pasture, and natural habitats","volume":"79","author":"Overzier","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Stuen, S., Granquist, E.G., and Silaghi, C. (2013). Anaplasma phagocytophilum\u2014A widespread multi-host pathogen with highly adaptive strategies. Front. Cell Infect. Microbiol., 3.","DOI":"10.3389\/fcimb.2013.00031"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"998","DOI":"10.3201\/eid1806.110997","article-title":"Wild boars as hosts of human-pathogenic Anaplasma phagocytophilum variants","volume":"18","author":"Michalik","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1196\/annals.1374.058","article-title":"Detection of Anaplasma phagocytophilum, Coxiella burnetii, Rickettsia spp., and Borrelia burgdorferi s. l. in Ticks, and wild-living animals in western and middle Slovakia","volume":"1078","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/j.1600-0463.2004.apm11204-0503.x","article-title":"Detection of Anaplasma phagocytophilum in animals by real-time polymerase chain reaction","volume":"112","year":"2004","journal-title":"Apmis"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1600-0463.2007.apm_256.x","article-title":"Detection and identification of Anaplasma phagocytophilum, Borrelia burgdorferi, and Rickettsia helvetica in Danish Ixodes ricinus ticks","volume":"115","author":"Skarphedinsson","year":"2007","journal-title":"Apmis"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"4172","DOI":"10.1128\/JCM.39.11.4172-4174.2001","article-title":"High prevalence of granulocytic Ehrlichiae and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Bulgaria","volume":"39","author":"Christova","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1186\/1756-3305-5-61","article-title":"Occurrence and identification of risk areas of Ixodes ricinus-borne pathogens: A cost-effectiveness analysis in north-eastern Italy","volume":"5","author":"Capelli","year":"2012","journal-title":"Parasit Vectors"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"3365","DOI":"10.1128\/jcm.35.12.3365-3366.1997","article-title":"Coexistence of Ehrlichia phagocytophila and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from Italy as determined by 16S rRNA gene sequencing","volume":"35","author":"Cinco","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.ttbdis.2012.01.002","article-title":"Seasonal activity and tick-borne pathogen infection rates of Ixodes ricinus ticks in Hungary","volume":"3","author":"Egyed","year":"2012","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"7594","DOI":"10.1128\/AEM.01851-06","article-title":"Longitudinal analysis of tick densities and Borrelia, Anaplasma, and Ehrlichia infections of Ixodes ricinus ticks in different habitat areas in The Netherlands","volume":"72","author":"Wielinga","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"2923","DOI":"10.1128\/AEM.03061-09","article-title":"Prevalence and seasonality of tick-borne pathogens in questing Ixodes ricinus ticks from Luxembourg","volume":"76","author":"Reye","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_80","first-page":"427","article-title":"Swiss Army Survey in Switzerland to determine the prevalence of Francisella tularensis, members of the Ehrlichia phagocytophila genogroup, Borrelia burgdorferi sensu lato, and tick-borne encephalitis virus in ticks","volume":"19","author":"Wicki","year":"2000","journal-title":"Eur. J. Clin."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1089\/vbz.2010.0215","article-title":"Pathogens of emerging tick-borne diseases, Anaplasma phagocytophilum, Rickettsia spp., and Babesia spp., in Ixodes ticks collected from rodents at four sites in Switzerland (Canton of Bern)","volume":"11","author":"Burri","year":"2011","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"3390","DOI":"10.1128\/JCM.37.10.3390-3391.1999","article-title":"Molecular evidence of coinfection of ticks with Borrelia burgdorferi sensu lato and the human granulocytic ehrlichiosis agent in Switzerland","volume":"37","author":"Leutenegger","year":"1999","journal-title":"J. Clin. Microbiol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/7\/612\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:55:32Z","timestamp":1760032532000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/7\/612"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,20]]},"references-count":82,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["pathogens14070612"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14070612","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,20]]}}}