{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T01:30:21Z","timestamp":1788571821200,"version":"build-2803163510"},"reference-count":52,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Faculty of Medicine, Thammasat University","award":["2-03\/2562"],"award-info":[{"award-number":["2-03\/2562"]}]},{"name":"Department of Pathobiology, Faculty of Science, Mahidol University","award":["2-03\/2562"],"award-info":[{"award-number":["2-03\/2562"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Malaria is a parasitic infectious disease that is endemic in many tropical countries. Even though several effective antimalarial agents have been implemented, treatment failure still occurs, and malaria continues to cause neurological complications and death, particularly in severe or drug-resistant cases. Hence, novel therapeutic agents with distinct mechanisms of action, as well as alternative chemical compounds that can overcome resistance, are still needed to improve malaria therapy. This study aimed to investigate the antimalarial activities of Brucea javanica, a tropical plant extracts against Plasmodium falciparum, the major species associated with severe malaria. In this study, malaria parasites were treated with plant extracts using single and co-incubation methods, along with artesunate and chloroquine, and their inhibitory effect on parasite development was determined by microscopy. The results show that all tested doses of the extracts that effectively inhibited malaria parasites did not cause hemolysis of red blood cells (RBCs). The root extract (RE) and fruit extract (FE) inhibited parasite growth at IC50 values of 0.41 \u00b1 1.14 \u00b5g\/mL and 0.26 \u00b1 1.15 \u00b5g\/mL, respectively. These plant extracts significantly interrupted malaria development at the ring stage, as presented by a reduction in the conversion rate to trophozoites and schizonts. The defective parasites treated with plant extracts were characterized by nuclear clumping, leading to pyknotic cell death. Moreover, RE and FW extracts elicited an additive effect with artesunate and chloroquine, significantly reducing IC90 levels for the inhibition of parasite development. In conclusion, B. javanica extracts inhibited the asexual blood-stage development of malaria parasites. They distinctively show the additive effects of ATS and CRQ, elucidating their potential for further studies on novel formulas of antimalarial drug regimens.<\/jats:p>","DOI":"10.3390\/pathogens14070646","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T06:56:54Z","timestamp":1751353014000},"page":"646","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Growth Inhibition and Additive Effect to Antimalarial Drugs of Brucea javanica Extracts on Asexual Blood-Stage Plasmodium falciparum"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6090-6689","authenticated-orcid":false,"given":"Niwat","family":"Kangwanrangsan","sequence":"first","affiliation":[{"name":"Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3694-6087","authenticated-orcid":false,"given":"Gamolthip","family":"Niramolyanun","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chonnipa","family":"Praikongkatham","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4974-9584","authenticated-orcid":false,"given":"Pathanin","family":"Chantree","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4168-5950","authenticated-orcid":false,"given":"Pongsakorn","family":"Martviset","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viriya","family":"Pankao","sequence":"additional","affiliation":[{"name":"Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani 12120, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2328","DOI":"10.1016\/S0140-6736(23)01249-7","article-title":"Malaria","volume":"402","author":"Poespoprodjo","year":"2023","journal-title":"Lancet"},{"key":"ref_2","unstructured":"World Health Organization (2024). World Malaria Report 2024: Addressing Inequity in the Global Malaria Response, World Health Organization."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1038\/nm.3073","article-title":"Malaria biology and disease pathogenesis: Insights for new treatments","volume":"19","author":"Miller","year":"2013","journal-title":"Nat. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1179\/2047773213Y.0000000084","article-title":"The pathogenesis of malaria: A new perspective","volume":"107","author":"Mawson","year":"2013","journal-title":"Pathog. Glob. Health"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1097\/QCO.0000000000000419","article-title":"Pathophysiology, clinical presentation, and treatment of coma and acute kidney injury complicating falciparum malaria","volume":"31","author":"Plewes","year":"2018","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"257","DOI":"10.4103\/0972-9062.316274","article-title":"Pretreatment gametocyte carriage in symptomatic patients with Plasmodium falciparum and Plasmodium vivax infections on the Thai-Myanmar border","volume":"58","author":"Martviset","year":"2021","journal-title":"J. Vector Borne Dis."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1038\/nrmicro2420","article-title":"Moving in and renovating: Exporting proteins from Plasmodium into host erythrocytes","volume":"8","author":"Goldberg","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.1093\/infdis\/jix230","article-title":"Parasites causing cerebral falciparum malaria bind multiple endothelial receptors and express EPCR and ICAM-1-binding PfEMP1","volume":"215","author":"Moussiliou","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Albrecht-Schgoer, K., Lackner, P., Schmutzhard, E., and Baier, G. (2022). Cerebral Malaria: Current Clinical and Immunological Aspects. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.863568"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1111\/febs.14127","article-title":"Combating multidrug-resistant Plasmodium falciparum malaria","volume":"284","author":"Thu","year":"2017","journal-title":"FEBS J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1007\/s40471-021-00266-5","article-title":"Antimalarial Drug Resistance and Implications for the WHO Global Technical Strategy","volume":"8","author":"Ippolito","year":"2021","journal-title":"Curr. Epidemiol. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1126\/science.1074045","article-title":"Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by pfcrt mutations","volume":"298","author":"Sidhu","year":"2002","journal-title":"Science"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1213","DOI":"10.1038\/s41564-023-01377-z","article-title":"Chloroquine resistance evolution in Plasmodium falciparum is mediated by the putative amino acid transporter AAT1","volume":"8","author":"Li","year":"2023","journal-title":"Nat. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1186\/s12936-016-1408-1","article-title":"Impact of using artemisinin-based combination therapy (ACT) in the treatment of uncomplicated malaria from Plasmodium falciparum in a non-endemic zone","volume":"15","year":"2016","journal-title":"Malar. J."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.actatropica.2005.06.009","article-title":"Artemisinin-based combination therapies (ACTs): Best hope for malaria treatment but inaccessible to the needy!","volume":"95","author":"Mutabingwa","year":"2005","journal-title":"Acta Trop."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Imwong, M., Jindakhad, T., Kunasol, C., Sutawong, K., Vejakama, P., and Dondorp, A.M. (2015). An outbreak of artemisinin resistant falciparum malaria in Eastern Thailand. Sci. Rep., 5.","DOI":"10.1038\/srep17412"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1186\/s12936-019-2903-y","article-title":"Molecular detection of drug resistant malaria in Southern Thailand","volume":"18","author":"Noisang","year":"2019","journal-title":"Malar. J."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Boonyalai, N., Thamnurak, C., Sai-ngam, P., Ta-aksorn, W., Arsanok, M., Uthaimongkol, N., Sundrakes, S., Chattrakarn, S., Chaisatit, C., and Praditpol, C. (2021). Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-92735-6"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1186\/s12936-022-04385-2","article-title":"Drug resistance of Plasmodium falciparum and Plasmodium vivax isolates in Indonesia","volume":"21","author":"Rahmasari","year":"2022","journal-title":"Malar. J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/s41579-024-01008-2","article-title":"Emergence, transmission dynamics and mechanisms of artemisinin partial resistance on malaria parasite in Africa","volume":"22","author":"Rosenthal","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e00109-24","DOI":"10.1128\/cmr.00109-24","article-title":"Artemisinin-resistant malaria","volume":"37","author":"White","year":"2024","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_22","first-page":"281","article-title":"Chinese Drugs of Plant Origin. Chemistry, Pharmacology, and Use in Traditional and Modern Medicine. Von W. Tang und G. Eisenbrand. Springer-Verlag Berlin etc. 1992, X, 1056, S., 41 Abb. gebd. DM 248,00","volume":"21","author":"Graf","year":"1992","journal-title":"Pharm. Unserer Zeit"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1007\/s11418-020-01407-8","article-title":"Quassilactones A and B, structural characterization of a new class of norquassinoids from Brucea javanica","volume":"74","author":"Su","year":"2020","journal-title":"J. Nat. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1080\/14786419.2013.821119","article-title":"A new quassinoid from fruits of Brucea javanica","volume":"27","author":"Su","year":"2013","journal-title":"Nat. Prod. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"918","DOI":"10.21037\/tcr.2019.12.62","article-title":"Brucea javanica oil emulsion suppresses tumor growth in human cervical cancer cells through inhibition of the E6 oncogene and induction of apoptosis","volume":"9","author":"Ye","year":"2020","journal-title":"Transl. Cancer Res."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Zhang, J., Xu, H.X., Dou, Y.X., Huang, Q.H., Xian, Y.F., and Lin, Z.X. (2022). Major Constituents From Brucea javanica and Their Pharmacological Actions. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.853119"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9867595","DOI":"10.1155\/2020\/9867595","article-title":"Nrf2 inhibitor, brusatol in combination with trastuzumab exerts synergistic antitumor activity in HER2-positive cancers by inhibiting Nrf2\/HO-1 and HER2-AKT\/ERK1\/2 pathways","volume":"2020","author":"Yang","year":"2020","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1093\/jpp\/rgab018","article-title":"An in vitro study on the reversal of epithelial to mesenchymal transition by brusatol and its synergistic properties in triple-negative breast cancer cells","volume":"73","author":"Chandrasekaran","year":"2021","journal-title":"J. Pharm. Pharmacol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.jare.2020.07.004","article-title":"Brusatol suppresses STAT3-driven metastasis by downregulating epithelial-mesenchymal transition in hepatocellular carcinoma","volume":"26","author":"Lee","year":"2020","journal-title":"J. Adv. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.fitote.2015.06.004","article-title":"Isolation, chemotaxonomic significance and cytotoxic effects of quassinoids from Brucea javanica","volume":"105","author":"Ye","year":"2015","journal-title":"Fitoterapia"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1002\/ps.1465","article-title":"Antiphytoviral activity of bruceine-D from Brucea javanica seeds","volume":"64","author":"Shen","year":"2008","journal-title":"Pest. Manag. Sci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"5887","DOI":"10.2147\/IJN.S174146","article-title":"Self-nanoemulsifying drug delivery system of bruceine D: A new approach for anti-ulcerative colitis","volume":"13","author":"Dou","year":"2018","journal-title":"Int. J. Nanomed."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"10556","DOI":"10.1002\/jcb.28341","article-title":"Brusatol inhibits amyloid-\u03b2-induced neurotoxicity in U-251 cells via regulating the Nrf2\/HO-1 pathway","volume":"120","author":"Liu","year":"2019","journal-title":"J. Cell Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1016\/j.bbrc.2020.03.097","article-title":"Bruceine D elevates Nrf2 activation to restrain Parkinson\u2019s disease in mice through suppressing oxidative stress and inflammatory response","volume":"526","author":"Yang","year":"2020","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1055\/s-2007-969092","article-title":"In vitro antimalarial activity of Brucea javanica against multi-drug resistant Plasmodium falciparum","volume":"52","author":"Pavanand","year":"1986","journal-title":"Planta Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3541","DOI":"10.1246\/bcsj.59.3541","article-title":"Structures of Yadanziosides K, M, N, and O, New Quassinoid Glycosides from Brucea javanica (L.) Merr","volume":"59","author":"Sakaki","year":"2006","journal-title":"Bull. Chem. Soc. Jpn."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1021\/np50049a007","article-title":"Plants as sources of antimalarial drugs, Part 4: Activity of Brucea javanica fruits against chloroquine-resistant Plasmodium falciparum in vitro and against Plasmodium berghei in vivo","volume":"50","author":"Bray","year":"1987","journal-title":"J. Nat. Prod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/lam.12085","article-title":"Brucin, an antibacterial peptide derived from fruit protein of Fructus Bruceae, Brucea javanica (L.) Merr","volume":"57","author":"Sornwatana","year":"2013","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_39","first-page":"1","article-title":"Phytochemical tested and in vitro screening anti- malaria activity of belilik (Brucea javanica (L)). MERR against Plasmodium falciparum","volume":"19","author":"Henny","year":"2014","journal-title":"Berk. Penelit. Hayati"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"61","DOI":"10.2149\/tmh.2009-11","article-title":"Antiplasmodial effects of Brucea javanica (L.) Merr. and Eurycoma longifolia Jack extracts and their combination with chloroquine and quinine on Plasmodium falciparum in culture","volume":"38","author":"Sriwilaijaroen","year":"2010","journal-title":"Trop. Med. Health"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/S0035-9203(97)90110-3","article-title":"An alternative to serum for cultivation of Plasmodium falciparum in vitro","volume":"91","author":"Cranmer","year":"1997","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"418","DOI":"10.2307\/3280287","article-title":"Synchronization of Plasmodium falciparum erythrocytic stages in culture","volume":"65","author":"Lambros","year":"1979","journal-title":"J. Parasitol."},{"key":"ref_43","first-page":"92","article-title":"A full-length protocol to test hemolytic activity of palytoxin on human erythrocytes D","volume":"4","author":"Malagoli","year":"2007","journal-title":"Invertebr. Surviv. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/1475-2875-13-63","article-title":"In vitro studies on the sensitivity pattern of Plasmodium falciparum to anti-malarial drugs and local herbal extracts","volume":"13","author":"Olasehinde","year":"2014","journal-title":"Malar. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.pt.2020.09.011","article-title":"Triple Artemisinin-Based Combination Therapies for Malaria\u2014A New Paradigm?","volume":"37","author":"Amaratunga","year":"2021","journal-title":"Trends Parasitol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"29","DOI":"10.11594\/jtls.02.02.01","article-title":"Acute toxicity studies of Brucea javanica Merril leaves extract on mice","volume":"2","author":"Angelina","year":"2012","journal-title":"J. Trop. Life Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1016\/j.parint.2017.09.002","article-title":"Medicinal plants for in vitro antiplasmodial activities: A systemic review of literature","volume":"66","author":"Lemma","year":"2017","journal-title":"Parasitol. Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"PM56","DOI":"10.1055\/s-0031-1282814","article-title":"Anti-proliferative Effect of Brucea javanica Fruit Extract Against Human Hepatocarcinoma Cell Lines and Its Mechanism","volume":"77","author":"Lu","year":"2011","journal-title":"Planta Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s12185-014-1605-z","article-title":"Effect of saponin on erythrocytes","volume":"100","author":"Bissinger","year":"2014","journal-title":"Int. J. Hematol."},{"key":"ref_50","first-page":"1","article-title":"Effects of antimalarial drugs on movement of Plasmodium falciparum","volume":"43","author":"Wongtanachai","year":"2012","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Thommen, B.T., Passecker, A., Buser, T., Hitz, E., Voss, T.S., and Brancucci, N.M.B. (2022). Revisiting the Effect of Pharmaceuticals on Transmission Stage Formation in the Malaria Parasite Plasmodium falciparum. Front. Cell Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.802341"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e0148121","DOI":"10.1128\/aac.01481-21","article-title":"Assessment In Vitro of the Antimalarial and Transmission-Blocking Activities of Cipargamin and Ganaplacide in Artemisinin-Resistant Plasmodium falciparum","volume":"66","author":"Yipsirimetee","year":"2022","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/7\/646\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:01:42Z","timestamp":1760032902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/7\/646"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,30]]},"references-count":52,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["pathogens14070646"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14070646","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,30]]}}}