{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:46:19Z","timestamp":1792806379053,"version":"4.5.0"},"reference-count":41,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T00:00:00Z","timestamp":1752969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Swiss Federal Food Safety and Veterinary Office (FSVO)","award":["# 1.20.i"],"award-info":[{"award-number":["# 1.20.i"]}],"id":[{"id":"https:\/\/ror.org\/01hwpsz06","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Small ruminant lentiviruses (SRLVs) are widespread and have a long co-evolutionary history with their hosts, namely sheep and goats. These viruses induce insidious pathologies, causing significant financial losses and animal welfare issues for the affected flocks. In Switzerland, in the 1980s, an eradication campaign was launched targeting these viruses, exclusively in goats, eliminating the virulent SRLV-B strains from the goat population, in which SRLV-B-induced arthritis was prevalent. Nevertheless, although they do not seem to induce clinical diseases, SRLV-A strains continue to circulate in Swiss goats. For this study, we contacted farmers who had animals testing positive for these strains during the census from 2011 to 2012 and visited six of these flocks, conducting serological, virological, and clinical analyses of the animals. We confirmed the absence of SRLV-B; however, we have detected SRLV-A in these flocks. Positive and negative animals lived in close contact for ten years and, except for a small flock of 13 animals, 7 of which tested positive, the transmission of these viruses proved inefficient. None of the positive animals showed any pathology attributable to SRLV infection. These encouraging results allowed us to formulate recommendations for the continued surveillance of these viruses in the Swiss goat population.<\/jats:p>","DOI":"10.3390\/pathogens14070719","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T09:33:53Z","timestamp":1753090433000},"page":"719","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Look Under the Carpet of a Successful Eradication Campaign Against Small Ruminant Lentiviruses"],"prefix":"10.3390","volume":"14","author":[{"given":"Fadri","family":"Vincenz","sequence":"first","affiliation":[{"name":"Veterinary Public Health Institute, Vetsuisse Faculty, University of Bern, 3097 Liebefeld, Switzerland"},{"name":"Institute of Virology and Immunology IVI, 3147 Bern, Switzerland"},{"name":"Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7030-637X","authenticated-orcid":false,"given":"Maksym","family":"Samoilenko","sequence":"additional","affiliation":[{"name":"Institute of Virology and Immunology IVI, 3147 Bern, Switzerland"},{"name":"Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Eduardo","family":"Abril","sequence":"additional","affiliation":[{"name":"Institute of Virology and Immunology IVI, 3147 Bern, Switzerland"},{"name":"Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9549-2487","authenticated-orcid":false,"given":"Patrik","family":"Zanolari","sequence":"additional","affiliation":[{"name":"Clinic for Ruminants, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1469-4723","authenticated-orcid":false,"given":"Giuseppe","family":"Bertoni","sequence":"additional","affiliation":[{"name":"Institute of Virology and Immunology IVI, 3147 Bern, Switzerland"},{"name":"Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8956-2231","authenticated-orcid":false,"given":"Beat","family":"Thomann","sequence":"additional","affiliation":[{"name":"Veterinary Public Health Institute, Vetsuisse Faculty, University of Bern, 3097 Liebefeld, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1007\/s007050050147","article-title":"Genomic heterogeneity of small ruminant lentiviruses: Existence of heterogeneous populations in sheep and of the same lentiviral genotypes in sheep and goats","volume":"142","author":"Leroux","year":"1997","journal-title":"Arch. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"94","DOI":"10.2174\/157016210790416415","article-title":"Srlvs: A genetic continuum of lentiviral species in sheep and goats with cumulative evidence of cross species transmission","volume":"8","author":"Leroux","year":"2010","journal-title":"Curr. HIV Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Olech, M., and Kuzmak, J. (2021). Molecular characterization of small ruminant lentiviruses in polish mixed flocks supports evidence of cross species transmission, dual infection, a recombination event, and reveals the existence of new subtypes within group a. Viruses, 13.","DOI":"10.3390\/v13122529"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3423","DOI":"10.1099\/vir.0.83292-0","article-title":"Genetic characterization of small ruminant lentivirus in italian mixed flocks: Evidence for a novel genotype circulating in a local goat population","volume":"88","author":"Grego","year":"2007","journal-title":"J. Gen. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Bouzalas, I., Apostolidi, E.D., Scalas, D., Davidopoulou, E., Chassalevris, T., Rosati, S., and Colitti, B. (2024). A combined approach for the characterization of small ruminant lentivirus strains circulating in the islands and mainland of greece. Animals, 14.","DOI":"10.3390\/ani14071119"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"93","DOI":"10.17236\/sat00196","article-title":"Diagnostic response to a cross-border challenge for the swiss caprine arthritis encephalitis virus eradication program","volume":"161","author":"Golomingi","year":"2019","journal-title":"Schweiz. Arch. Tierheilkd."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1126\/science.6153243","article-title":"Chronic arthritis in goats caused by a retrovirus","volume":"207","author":"Crawford","year":"1980","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.prevetmed.2016.12.012","article-title":"A census to determine the prevalence and risk factors for caprine arthritis-encephalitis virus and visna\/maedi virus in the swiss goat population","volume":"137","author":"Thomann","year":"2017","journal-title":"Prev. Vet. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1186\/1743-422X-11-65","article-title":"Characterization of small ruminant lentivirus a4 subtype isolates and assessment of their pathogenic potential in naturally infected goats","volume":"11","author":"Deubelbeiss","year":"2014","journal-title":"Virol. J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.vetmic.2012.11.017","article-title":"Virological and phylogenetic characterization of attenuated small ruminant lentivirus isolates eluding efficient serological detection","volume":"162","author":"Cardinaux","year":"2013","journal-title":"Vet. Microbiol"},{"key":"ref_11","unstructured":"(2023, April 28). Swiss Federal Food Safety and Veterinary Office (FSVO): 2015\u20132022. \u00abTiergesundheitsstatistik\u00bb. Available online: https:\/\/www.blv.admin.ch\/blv\/de\/home\/tiere\/publikationen\/statistiken-berichte-tiere.html."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Schaer, J., Cvetnic, Z., Sukalic, T., Dorig, S., Grisiger, M., Iscaro, C., Feliziani, F., Pfeifer, F., Origgi, F., and Zanoni, R.G. (2022). Evaluation of serological methods and a new real-time nested pcr for small ruminant lentiviruses. Pathogens, 11.","DOI":"10.3390\/pathogens11020129"},{"key":"ref_13","unstructured":"Rackwitz, R., Pees, M., Aschenbach, J.R., and G\u00e4bel, G. (2014). Su5 serology as a novel tool to support a challenging caprine arthritis encephalitis virus (caev) eradication campaign. Lbh: 7. Leipziger Tier\u00e4rztekongress, University of Leipzig."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1128\/JCM.44.3.981-991.2006","article-title":"Analysis of the antibody response to an immunodominant epitope of the envelope glycoprotein of a lentivirus and its diagnostic potential","volume":"44","author":"Mordasini","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1136\/vr.157.8.229","article-title":"Risk factors for the infection of swiss goat herds with small ruminant lentivirus: A case-control study","volume":"157","author":"Vogt","year":"2005","journal-title":"Vet. Rec."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"511","DOI":"10.2478\/jvetres-2022-0071","article-title":"Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus-infected dams","volume":"66","author":"Kaba","year":"2022","journal-title":"J. Vet. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.virol.2010.08.004","article-title":"Genetic analysis of small ruminant lentiviruses following lactogenic transmission","volume":"407","author":"Pisoni","year":"2010","journal-title":"Virology"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"106297","DOI":"10.1016\/j.prevetmed.2024.106297","article-title":"Maternal transmission of small ruminant lentivirus has no epidemiological importance","volume":"230","author":"Illius","year":"2024","journal-title":"Prev. Vet. Med."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s13567-015-0162-7","article-title":"Genetic subgroup of small ruminant lentiviruses that infects sheep homozygous for tmem154 frameshift deletion mutation a4delta53","volume":"46","author":"Clawson","year":"2015","journal-title":"Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1186\/1743-422X-8-271","article-title":"Molecular characterization and phylogenetic analysis of small ruminant lentiviruses isolated from canadian sheep and goats","volume":"8","author":"Ouardani","year":"2011","journal-title":"Virol. J."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zahno, M.L., and Bertoni, G. (2018). An immunodominant region of the envelope glycoprotein of small ruminant lentiviruses may function as decoy antigen. Viruses, 10.","DOI":"10.3390\/v10050231"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.virol.2006.12.021","article-title":"Evidence of proviral clearance following postpartum transmission of an ovine lentivirus","volume":"362","author":"Palmer","year":"2007","journal-title":"Virology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.vetmic.2012.12.004","article-title":"Small ruminant lentivirus genotype b and e interaction: Evidences on the role of roccaverano strain on reducing proviral load of the challenging caev strain","volume":"163","author":"Bertolotti","year":"2012","journal-title":"Vet. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1016\/j.jtbi.2019.02.018","article-title":"An effective management strategy for the control of two lentiviruses in goat breedings","volume":"469","author":"Venturino","year":"2019","journal-title":"J. Theor. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Brotto Rebuli, K., Giacobini, M., and Bertolotti, L. (2021). Caprine arthritis encephalitis virus disease modelling review. Animals, 11.","DOI":"10.3390\/ani11051457"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Samoilenko, M., Nedosekov, V., and Bertoni, G. (2025). Testing the tenacity of small ruminant lentiviruses in vitro to assess the potential risk of indirect fomites\u2019 transmission. Viruses, 17.","DOI":"10.3390\/v17030419"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"104905","DOI":"10.1016\/j.prevetmed.2020.104905","article-title":"Seroprevalence of small ruminant lentivirus (srlv) infection in wild cervids in poland","volume":"176","author":"Olech","year":"2020","journal-title":"Prev. Vet. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1177\/1040638711435503","article-title":"Fatal caprine arthritis encephalitis virus-like infection in 4 rocky mountain goats (Oreamnos americanus)","volume":"24","author":"Patton","year":"2012","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1478","DOI":"10.1099\/vir.0.2008\/000364-0","article-title":"Small ruminant lentivirus proviral sequences from wild ibexes in contact with domestic goats","volume":"89","author":"Erhouma","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Molaee, V., Bazzucchi, M., De Mia, G.M., Otarod, V., Abdollahi, D., Rosati, S., and Luhken, G. (2020). Phylogenetic analysis of small ruminant lentiviruses in germany and iran suggests their expansion with domestic sheep. Sci. Rep., 10.","DOI":"10.1038\/s41598-020-58990-9"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7518","DOI":"10.1128\/JVI.78.14.7518-7522.2004","article-title":"Direct evidence for natural transmission of small-ruminant lentiviruses of subtype a4 from goats to sheep and vice versa","volume":"78","author":"Shah","year":"2004","journal-title":"J. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.rvsc.2004.09.006","article-title":"Relative contribution of colostrum from maedi-visna virus (mvv) infected ewes to mvv-seroprevalence in lambs","volume":"78","author":"Alvarez","year":"2005","journal-title":"Res. Vet. Sci."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/S0034-5288(18)32917-5","article-title":"Studies in epidemiology of maedi\/visna in sheep","volume":"26","author":"Terpstra","year":"1979","journal-title":"Res. Vet. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.vetmic.2015.08.007","article-title":"Small ruminant lentivirus infections and diseases","volume":"181","author":"Minguijon","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"479","DOI":"10.2478\/jvetres-2023-0064","article-title":"The genetic variability of small-ruminant lentiviruses and its impact on tropism, the development of diagnostic tests and vaccines and the effectiveness of control programmes","volume":"67","author":"Olech","year":"2023","journal-title":"J. Vet. Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1051\/vetres:2006034","article-title":"Extensive rearing hinders maedi-visna virus (mvv) infection in sheep","volume":"37","author":"Leginagoikoa","year":"2006","journal-title":"Vet. Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.virusres.2006.12.014","article-title":"Natural transmission and comparative analysis of small ruminant lentiviruses in the norwegian sheep and goat populations","volume":"125","author":"Gjerset","year":"2007","journal-title":"Virus Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.virol.2005.06.013","article-title":"Phylogenetic analysis of small-ruminant lentivirus subtype b1 in mixed flocks: Evidence for natural transmission from goats to sheep","volume":"339","author":"Pisoni","year":"2005","journal-title":"Virology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.3390\/v5082043","article-title":"Retroviral infections in sheep and goats: Small ruminant lentiviruses and host interaction","volume":"5","author":"Larruskain","year":"2013","journal-title":"Viruses"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Heaton, M.P., Clawson, M.L., Chitko-McKown, C.G., Leymaster, K.A., Smith, T.P., Harhay, G.P., White, S.N., Herrmann-Hoesing, L.M., Mousel, M.R., and Lewis, G.S. (2012). Reduced lentivirus susceptibility in sheep with tmem154 mutations. PLoS Genet., 8.","DOI":"10.1371\/journal.pgen.1002467"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1099\/vir.0.044768-0","article-title":"Diverse host-virus interactions following caprine arthritis-encephalitis virus infection in sheep and goats","volume":"94","author":"Rachid","year":"2013","journal-title":"J. Gen. Virol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/7\/719\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:12:54Z","timestamp":1760033574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/7\/719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,20]]},"references-count":41,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["pathogens14070719"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14070719","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,20]]}}}