{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T07:21:18Z","timestamp":1766647278042,"version":"build-2065373602"},"reference-count":63,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T00:00:00Z","timestamp":1753401600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnolo\u0301gico (CNPq)","award":["APQ-01629-22","001"],"award-info":[{"award-number":["APQ-01629-22","001"]}]},{"name":"Fundac\u0327\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","award":["APQ-01629-22","001"],"award-info":[{"award-number":["APQ-01629-22","001"]}]},{"name":"Coordenac\u0327\u00e3o de Aperfeic\u0327oamento de Pessoal de N\u0131vel Superior\u2019\u2014Brazil (CAPES)","award":["APQ-01629-22","001"],"award-info":[{"award-number":["APQ-01629-22","001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Congenital Chagas disease (CCD) is caused when Trypanosoma cruzi crosses the placental barrier during pregnancy and reaches the fetus, which can lead to serious consequences in the developing fetus. Current treatment is carried out with nifurtimox or benznidazole, but their effectiveness is limited, and they cause side effects, requiring the search for new therapeutic strategies. In this sense, many studies have demonstrated the potential of different compounds of the Copaifera genus in the control of parasitic diseases. Here, we aimed to evaluate the effect of oleoresin (OR) and leaf hydroalcoholic extract (LHE) of Copaifera multijuga on Trypanosoma cruzi infection in human villous trophoblast cells (BeWo line) and human placenta explants. Treatment with both compounds reduced invasion, proliferation, and release of trypomastigotes. Furthermore, OR and LHE affected the trypomastigotes and amastigote morphology, compromising their ability to invade and proliferate in BeWo cells, respectively. Also, treatment with OR decreased ROS production in infected BeWo cells, while LHE induced an increase. In addition, both compounds induced pro-inflammatory and anti-inflammatory cytokine production. In human placental explants, both compounds also decreased T. cruzi infection, in addition to inducing the production of pro-inflammatory cytokines. Thus, both OR and LHE of C. multijuga control T. cruzi infection at the human maternal\u2013fetal interface, highlighting the possible therapeutic potential of these compounds for the treatment of CCD.<\/jats:p>","DOI":"10.3390\/pathogens14080736","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T12:56:43Z","timestamp":1753448203000},"page":"736","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Trypanosoma cruzi Growth Is Impaired by Oleoresin and Leaf Hydroalcoholic Extract from Copaifera multijuga in Human Trophoblast and Placental Explants"],"prefix":"10.3390","volume":"14","author":[{"given":"Guilherme de","family":"Souza","sequence":"first","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara Peleteiro","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6886-281X","authenticated-orcid":false,"given":"Joed Pires de","family":"Lima J\u00fanior","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3835-1925","authenticated-orcid":false,"given":"Marcos Paulo Oliveira","family":"Almeida","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Paschoalino","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luana Carvalho","family":"Luz","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia Carine Lima","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1608-9172","authenticated-orcid":false,"given":"Rafael Martins","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izadora Santos","family":"Damasceno","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1856-0335","authenticated-orcid":false,"given":"Matheus Carvalho","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilherme Vieira","family":"Faria","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Anita Lemos Vasconcelos","family":"Ambrosio","sequence":"additional","affiliation":[{"name":"Nucleus of Research in Technological and Exact Sciences, Universidade de Franca, Franca 14404-600, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Cassio Sola","family":"Veneziani","sequence":"additional","affiliation":[{"name":"Nucleus of Research in Technological and Exact Sciences, Universidade de Franca, Franca 14404-600, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8641-9686","authenticated-orcid":false,"given":"Jairo Kenupp","family":"Bastos","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences of Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto 14040-903, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8719-2794","authenticated-orcid":false,"given":"Angelica Oliveira","family":"Gomes","sequence":"additional","affiliation":[{"name":"Institute of Natural and Biological Sciences, Universidade Federal do Tri\u00e2ngulo Mineiro, Uberaba 38025-015, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7470-7082","authenticated-orcid":false,"given":"Rosiane Nascimento","family":"Alves","sequence":"additional","affiliation":[{"name":"Department of Agricultural and Natural Science, Universidade do Estado de Minas Gerais, Ituiutaba 38302-192, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Henrique Gomes","family":"Martins","sequence":"additional","affiliation":[{"name":"Laboratory of Antimicrobial Testing, Institute of Biomedical Sciences, Universidade Federal de Uberlandia, Uberlandia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel Cota","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7447-2583","authenticated-orcid":false,"given":"Eloisa Am\u00e1lia Vieira","family":"Ferro","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4611-2844","authenticated-orcid":false,"given":"Bellisa Freitas","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Laboratory of Immunophysiology of Reproduction, Institute of Biomedical Science, Universidade Federal de Uberl\u00e2ndia, Uberl\u00e2ndia 38405-318, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,25]]},"reference":[{"key":"ref_1","first-page":"203","article-title":"Chagas disease","volume":"10422","author":"Vermeij","year":"2024","journal-title":"Lancet"},{"key":"ref_2","first-page":"100881","article-title":"The epidemiology of Chagas disease in the Americas","volume":"37","author":"Ceccarelli","year":"2024","journal-title":"Lancet Reg. 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