{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:57:37Z","timestamp":1775869057346,"version":"3.50.1"},"reference-count":24,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Natural Science Foundation of Heilongjiang","award":["JQ2022C006"],"award-info":[{"award-number":["JQ2022C006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The apicoplast is a highly specialized organelle in the biosynthesis of essential metabolites in most of the apicomplexan protozoa. This organelle is surrounded by four layers of membranes. However, the molecular mechanisms mediating transmembrane transport are not yet fully understood. In this study, we conducted a phenotypic analysis to investigate the role of a major facilitator superfamily transporter (TgApMFS1) in the survival of the parasite. The results indicated that TgApMFS1 is critical for the survival of Toxoplasma gondii in cell culture conditions. Further analysis indicated that these transporters are crucial for the biogenesis of organelles and the metabolic processes of parasite.<\/jats:p>","DOI":"10.3390\/pathogens14080763","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T07:45:11Z","timestamp":1754466311000},"page":"763","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Major Facilitator Superfamily Transporter Is Critical for the Metabolism and Biogenesis of the Apicoplast"],"prefix":"10.3390","volume":"14","author":[{"given":"Yumeng","family":"Liang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiawen","family":"Fu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7458-9565","authenticated-orcid":false,"given":"Honglin","family":"Jia","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"},{"name":"Heilongjiang Research Center for Veterinary Biopharmaceutical Technology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1111\/j.1550-7408.2008.00345.x","article-title":"The history of Toxoplasma gondii\u2014The first 100 years","volume":"55","author":"Dubey","year":"2008","journal-title":"J. Eukaryot. Microbiol."},{"key":"ref_2","first-page":"165","article-title":"Global initiative for congenital toxoplasmosis: An observational and international comparative clinical analysis","volume":"7","author":"Levigne","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/j.mib.2021.07.016","article-title":"The metabolic pathways and transporters of the plastid organelle in Apicomplexa","volume":"63","author":"Kloehn","year":"2021","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1144","DOI":"10.1016\/j.pt.2024.10.019","article-title":"The apicoplast biogenesis and metabolism: Current progress and questions","volume":"40","author":"Chen","year":"2024","journal-title":"Trends Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.chom.2009.12.002","article-title":"The toxoplasma apicoplast phosphate translocator links cytosolic and apicoplast metabolism and is essential for parasite survival","volume":"7","author":"Brooks","year":"2010","journal-title":"Cell Host Microbe"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5802","DOI":"10.1038\/s41467-021-25987-5","article-title":"A plastid two-pore channel essential for inter-organelle communication and growth of Toxoplasma gondii","volume":"12","author":"Li","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1128\/EC.00061-07","article-title":"Carbohydrate metabolism in the Toxoplasma gondii apicoplast: Localization of three glycolytic isoenzymes, the single pyruvate dehydrogenase complex, and a plastid phosphate translocator","volume":"6","author":"Fleige","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1111\/j.1365-2958.2007.05619.x","article-title":"Cell cycle-regulated vesicular trafficking of Toxoplasma APT1, a protein localized to multiple apicoplast membranes","volume":"63","author":"Karnataki","year":"2007","journal-title":"Mol. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/j.febslet.2009.11.097","article-title":"The carbon and energy sources of the non-photosynthetic plastid in the malaria parasite","volume":"584","author":"Lim","year":"2010","journal-title":"FEBS. Lett."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"9572","DOI":"10.1073\/pnas.0602293103","article-title":"Membrane transporters in the relict plastid of malaria parasites","volume":"103","author":"Mullin","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"RP88866","DOI":"10.7554\/eLife.88866.3","article-title":"The Toxoplasma monocarboxylate transporters are involved in the metabolism within the apicoplast and are linked to parasite survival","volume":"12","author":"Dong","year":"2024","journal-title":"Elife"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e2314314121","DOI":"10.1073\/pnas.2314314121","article-title":"A pyruvate transporter in the apicoplast of apicomplexan parasites","volume":"121","author":"Chen","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Chen, X., Tang, T., Ding, H., Dong, H., Long, S., and Suo, X. (2025). The Major Facilitator Superfamily Transporter HAP12 Is Critical in Toxoplasma gondii Survival and Virulence. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26083910"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1111\/mmi.14411","article-title":"The Sec1\/Munc18-like proteins TgSec1 and TgVps45 play pivotal roles in assembly of the pellicle and sub-pellicle network in Toxoplasma gondii","volume":"113","author":"Cao","year":"2020","journal-title":"Mol. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Cao, S., Yang, J., Fu, J., Chen, H., and Jia, H. (2021). The Dissection of SNAREs Reveals Key Factors for Vesicular Trafficking to the Endosome-like Compartment and Apicoplast via the Secretory System in Toxoplasma gondii. mBio, 12.","DOI":"10.1128\/mBio.01380-21"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Zheng, J., Cheng, Z., Jia, H., and Zheng, Y. (2016). Characterization of aspartyl aminopeptidase from Toxoplasma gondii. Sci. Rep., 6.","DOI":"10.1038\/srep34448"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1080\/15548627.2022.2123639","article-title":"Apicoplast biogenesis mediated by ATG8 requires the ATG12-ATG5-ATG16L and SNAP29 complexes in Toxoplasma gondii","volume":"19","author":"Fu","year":"2023","journal-title":"Autophagy"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.chom.2020.09.011","article-title":"A Comprehensive Subcellular Atlas of the Toxoplasma Proteome via hyperLOPIT Provides Spatial Context for Protein Functions","volume":"28","author":"Barylyuk","year":"2020","journal-title":"Cell Host Microbe"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1016\/j.cell.2016.08.019","article-title":"A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes","volume":"166","author":"Sidik","year":"2016","journal-title":"Cell"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1016\/S1369-5274(99)80075-7","article-title":"Origin, targeting, and function of the apicomplexan plastid","volume":"2","author":"Roos","year":"1999","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1111\/j.1600-0854.2012.01335.x","article-title":"Apicoplast targeting of a Toxoplasma gondii transmembrane protein requires a cytosolic tyrosine-based motif","volume":"13","author":"DeRocher","year":"2012","journal-title":"Traffic"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1111\/boc.202000050","article-title":"Dually localised proteins found in both the apicoplast and mitochondrion utilize the Golgi-dependent pathway for apicoplast targeting in Toxoplasma gondii","volume":"113","author":"Prasad","year":"2021","journal-title":"Biol. Cell"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Jia, H., Cao, S., Yang, J., and Fu, J. (2021). Reply to Prasad and Patankar, \u201cRecognition of Two Distinct Pathways for Trafficking of Proteins to the Apicoplast\u201d. mBio, 12.","DOI":"10.1128\/mBio.03107-21"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5289","DOI":"10.1021\/acs.chemrev.0c00983","article-title":"Structures and General Transport Mechanisms by the Major Facilitator Superfamily (MFS)","volume":"121","author":"Drew","year":"2021","journal-title":"Chem. Rev."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/8\/763\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:21:27Z","timestamp":1760034087000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/8\/763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,1]]},"references-count":24,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["pathogens14080763"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14080763","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,1]]}}}