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Technology (EU-COST)","award":["NUHSRO\/2018\/006\/SU\/01"],"award-info":[{"award-number":["NUHSRO\/2018\/006\/SU\/01"]}]},{"name":"European Cooperation in Science &amp; Technology (EU-COST)","award":["NUHSRO\/2019\/046\/PDF\/19"],"award-info":[{"award-number":["NUHSRO\/2019\/046\/PDF\/19"]}]},{"name":"European Cooperation in Science &amp; Technology (EU-COST)","award":["A-8000685-00-00"],"award-info":[{"award-number":["A-8000685-00-00"]}]},{"name":"European Cooperation in Science &amp; Technology (EU-COST)","award":["A-8000629-00-00"],"award-info":[{"award-number":["A-8000629-00-00"]}]},{"name":"European Cooperation in Science &amp; Technology (EU-COST)","award":["OC-2021-1-25116"],"award-info":[{"award-number":["OC-2021-1-25116"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Blastocystis spp. is an enteric protist that is present worldwide. Despite being discovered a century ago, there is still much to be learned about its pathogenicity and transmission. Different subtypes (ST) of Blastocystis spp. have been identified in various hosts, including humans, birds, and insects, and there is potential for zoonotic transmission through contact between humans and animals. The prevalence of Blastocystis spp. in humans and macaques in Singapore was understudied, and the findings revealed a significant prevalence of the parasite, with rates of 90% and 100% observed in each respective Macaca fascicularis population 1 and 2, with main subtypes (ST1, ST2, ST3, and ST5). Using metagenomics, the different subtypes of Blastocystis spp. (comprising ST2, ST3, and ST17) were identified in a healthy Singaporean cohort. Additionally, seven incidental findings of Blastocystis spp. were discovered in human patients with other gut parasites, including two ST1, two ST2, two ST3, and one ST8. Several factors such as diet or reverse zoonotic transmission are suggested to play a role in Blastocystis sp. subtype distribution.<\/jats:p>","DOI":"10.3390\/pathogens14080773","type":"journal-article","created":{"date-parts":[[2025,8,6]],"date-time":"2025-08-06T07:45:11Z","timestamp":1754466311000},"page":"773","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Assessing Zoonotic Risks of Blastocystis Infection in Singapore"],"prefix":"10.3390","volume":"14","author":[{"given":"Thet Tun","family":"Aung","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Immunology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore"},{"name":"Singapore Eye Research Institute, Singapore 169856, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte Kai Qi","family":"How","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Immunology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0003-541X","authenticated-orcid":false,"given":"Jean-Marc","family":"Chavatte","sequence":"additional","affiliation":[{"name":"National Public Health Laboratory, National Centre for Infectious Diseases, Singapore 308442, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9481-2511","authenticated-orcid":false,"given":"Nazmi Bin","family":"Nazir","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Immunology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar Macabe","family":"Pena","sequence":"additional","affiliation":[{"name":"SingHealth Experimental Medicine Centre and National Large Animal Research Facility, Singapore 169856, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan","family":"Ogden","sequence":"additional","affiliation":[{"name":"SingHealth Experimental Medicine Centre and National Large Animal Research Facility, Singapore 169856, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace Rou\u2019en","family":"Lim","sequence":"additional","affiliation":[{"name":"Comparative Medicine, National University of Singapore, Singapore 119077, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasmina Arditi","family":"Paramastri","sequence":"additional","affiliation":[{"name":"Comparative Medicine, National University of Singapore, Singapore 119077, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lois Anne","family":"Zitzow","sequence":"additional","affiliation":[{"name":"Comparative Medicine, National University of Singapore, Singapore 119077, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanrong","family":"Chen","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore, A*Star, Singapore 138672, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niranjan","family":"Nagarajan","sequence":"additional","affiliation":[{"name":"Genome Institute of Singapore, A*Star, Singapore 138672, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin Shyong Wei","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Healthy Longevity Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9658-7528","authenticated-orcid":false,"given":"Benoit","family":"Malleret","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Immunology Translational Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117545, Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1756-3305-1-40","article-title":"Oh my aching gut: Irritable bowel syndrome, Blastocystis, and asymptomatic infection","volume":"1","author":"Boorom","year":"2008","journal-title":"Parasites Vectors"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Cian, A., El Safadi, D., Osman, M., Moriniere, R., Gantois, N., Benamrouz-Vanneste, S., Delgado-Viscogliosi, P., Guyot, K., Li, L.-L., and Monchy, S. 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