{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T09:35:16Z","timestamp":1783762516597,"version":"3.55.0"},"reference-count":49,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T00:00:00Z","timestamp":1755043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Aim: The aim of the current investigation was to detect serotypes of Aggregatibacter actinomycetemcomitans in a cohort of Western Australians diagnosed with periodontitis. Materials and Methods: A total of 64 subjects were selected. Intra-oral samples were taken from every subject in the present investigation. Periodontal, radiographical, and microbiological analyses were conducted. A polymerase chain reaction was employed to investigate the presence of Aggregatibacter actinomycetemcomitans serotypes. Results: Only twelve (18.75%) patients were tested positive for Aggregatibacter actinomycetemcomitans. The most dominant serotypes of Aggregatibacter actinomycetemcomitans in this group were serotype e (80.55%), followed by serotype c (52.77%). Both serotypes b and d were absent in the present investigation. Serotype e presented in isolation or combined with other serotypes. The other serotypes tend to be present alone, but when they were isolated together, they were always combined with serotype e. It seems that serotype e of Aggregatibacter actinomycetemcomitans is associated with those who live in rural areas (p = 0.003), and those with low education (p = 0.041), and severe forms of periodontitis in this cohort. Conclusions: In patients diagnosed with severe periodontitis, serotype e was dominant in this population. Serotypes b and d did not appear in the present study.<\/jats:p>","DOI":"10.3390\/pathogens14080805","type":"journal-article","created":{"date-parts":[[2025,8,13]],"date-time":"2025-08-13T15:53:27Z","timestamp":1755100407000},"page":"805","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Serotype Distribution of Aggregatibacter actinomycetemcomitans in Periodontitis Patients"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8862-6427","authenticated-orcid":false,"given":"Nabil","family":"Khzam","sequence":"first","affiliation":[{"name":"Dental School, The University of Western Australia, Nedlands, WA 6009, Australia"},{"name":"NK Periodontics, Specialist Periodontal Private Practice, Applecross, WA 6155, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5951-8280","authenticated-orcid":false,"given":"Omar","family":"Kujan","sequence":"additional","affiliation":[{"name":"Dental School, The University of Western Australia, Nedlands, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9983-8767","authenticated-orcid":false,"given":"Dorte","family":"Haubek","sequence":"additional","affiliation":[{"name":"Jammerbugt Municipal Dental Service, DK-9460 Brovst, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6796-693X","authenticated-orcid":false,"given":"Leticia Algarves","family":"Miranda","sequence":"additional","affiliation":[{"name":"Dental School, The University of Western Australia, Nedlands, WA 6009, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/prd.12362","article-title":"Microbial signatures of health, gingivitis, and periodontitis","volume":"86","author":"Abusleme","year":"2021","journal-title":"Periodontol. 2000"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1111\/jcpe.12534","article-title":"Clinical research activity in periodontal medicine: A systematic mapping of trial registers","volume":"43","author":"Monsarrat","year":"2016","journal-title":"J. Clin. Periodontol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/prd.12616","article-title":"Periodontal disease: A systemic condition","volume":"96","author":"Villoria","year":"2024","journal-title":"Periodontology 2000"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/prd.12377","article-title":"Microbial transitions from health to disease","volume":"86","author":"Joseph","year":"2021","journal-title":"Periodontology 2000"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1111\/j.1600-0757.2009.00331.x","article-title":"Aggregatibacter (Actinobacillus) actinomycetemcomitans: A triple A* periodontopathogen?","volume":"54","author":"Henderson","year":"2010","journal-title":"Periodontol. 2000"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1600-051X.1985.tb01348.x","article-title":"Actinobacillus actinomycetemcomitans in human periodontal disease","volume":"12","author":"Zambon","year":"1985","journal-title":"J. Clin. Periodontol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1128\/iai.25.1.427-439.1979","article-title":"Extraction and partial characterization of a leukotoxin from a plaque-derived Gram-negative microorganism","volume":"25","author":"Tsai","year":"1979","journal-title":"Infect. Immun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1128\/iai.41.1.19-27.1983","article-title":"Serology of oral Actinobacillus actinomycetemcomitans and serotype distribution in human periodontal disease","volume":"41","author":"Zambon","year":"1983","journal-title":"Infect. Immun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/omi.12005","article-title":"Comparative genomic hybridization and transcriptome analysis with a pan-genome microarray reveal distinctions between JP2 and non-JP2 genotypes of Aggregatibacter actinomycetemcomitans","volume":"28","author":"Huang","year":"2013","journal-title":"Mol. Oral. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1128\/JCM.40.4.1181-1187.2002","article-title":"Population Structure and Genetic Diversity of Actinobacillus actinomycetemcomitans Strains Isolated from Localized Juvenile Periodontitis Patients","volume":"40","author":"Kaplan","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/j.1600-0757.2006.00159.x","article-title":"The etiology of periodontal disease revisited by population genetic analysis","volume":"42","author":"Kilian","year":"2006","journal-title":"Periodontology 2000"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1128\/JCM.42.2.807-810.2004","article-title":"Emergence of nonencapsulated and encapsulated non-b-type invasive Haemophilus influenzae isolates in Portugal (1989\u20132001)","volume":"42","author":"Bajanca","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1128\/jcm.29.11.2489-2493.1991","article-title":"Emergence of a virulent clone of Neisseria meningitidis serotype 2a that is associated with meningococcal group C disease in Canada","volume":"29","author":"Ashton","year":"1991","journal-title":"J. Clin. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1600-0757.1999.tb00160.x","article-title":"Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in nonoral infections","volume":"20","author":"Slots","year":"1999","journal-title":"Periodontology 2000"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1902\/jop.2009.090241","article-title":"Serotypes of Aggregatibacter actinomycetemcomitans in patients with different ethnic backgrounds","volume":"80","author":"Kim","year":"2009","journal-title":"J. Periodontol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1128\/iai.62.2.501-508.1994","article-title":"Regulation of Actinobacillus actinomycetemcomitans leukotoxin expression: Analysis of the promoter regions of leukotoxic and minimally leukotoxic strains","volume":"62","author":"Brogan","year":"1994","journal-title":"Infect. Immun."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1177\/00220345010800062001","article-title":"Early-onset periodontitis in Morocco is associated with the highly leukotoxic clone of Actinobacillus actinomycetemcomitans","volume":"80","author":"Haubek","year":"2001","journal-title":"J. Dent. Res."},{"key":"ref_18","first-page":"e184","article-title":"Serotypes of Aggregatibacter actinomycetemcomitans in relation to periodontal status and geographic origin of individuals-a review of the literature","volume":"19","author":"Rego","year":"2014","journal-title":"Med. Oral. Patol. Oral. Cir. Bucal"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/j.1600-0765.1997.tb00578.x","article-title":"Prevalence of Actinobacillus actinomycetemcomitans serotypes in Japanese patients with periodontitis","volume":"32","author":"Yamamoto","year":"1997","journal-title":"J. Periodontal Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"994","DOI":"10.1111\/j.1600-051X.1998.tb02404.x","article-title":"Analysis of serum antibody responses to periodontopathogens in early-onset periodontitis patients from different geographical locations","volume":"25","author":"Celenligil","year":"1998","journal-title":"J. Clin. Periodontol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1111\/jcpe.12296","article-title":"IgG sera levels against a subset of periodontopathogens and severity of disease in aggressive periodontitis patients: A cross-sectional study of selected pocket sites","volume":"41","author":"Saraiva","year":"2014","journal-title":"J. Clin. Periodontol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1111\/j.1399-302X.1992.tb00588.x","article-title":"Frequency and stability of mono- or poly-infection by Actinobacillus actinomycetemcomitans serotypes a, b, c, d or e","volume":"7","author":"Saarela","year":"1992","journal-title":"Oral. Microbiol. Immunol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/j.1399-302X.2007.00354.x","article-title":"Identification of periodontopathic bacteria in gingival tissue of Japanese periodontitis patients","volume":"22","author":"Thiha","year":"2007","journal-title":"Oral. Microbiol. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1111\/j.1600-051X.2008.01280.x","article-title":"Prevalence and distribution of principal periodontal pathogens worldwide","volume":"35","author":"Rylev","year":"2008","journal-title":"J. Clin. Periodontol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1111\/j.1600-051X.2012.01871.x","article-title":"Aggregatibacter actinomycetemcomitans serotypes, the JP2 clone and cytolethal distending toxin genes in a Thai population","volume":"39","author":"Bandhaya","year":"2012","journal-title":"J. Clin. Periodontol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Claesson, R., H\u00f6glund-\u00c5berg, C., Haubek, D., and Johansson, A. (2017). Age-related prevalence and characteristics of Aggregatibacter actinomycetemcomitans in periodontitis patients living in Sweden. J. Oral. Microbiol., 9.","DOI":"10.1080\/20002297.2017.1334504"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Khzam, N., Kujan, O., Haubek, D., Arslan, A., Johansson, A., Oscarsson, J., Razooqi, Z., and Miranda, L.A. (2024). Prevalence of Subgingival Aggregatibacter actinomycetemcomitans: Descriptive Cross-Sectional Study. Pathogens, 13.","DOI":"10.3390\/pathogens13070531"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"S159","DOI":"10.1002\/JPER.18-0006","article-title":"Staging and grading of periodontitis: Framework and proposal of a new classification and case definition","volume":"89","author":"Tonetti","year":"2018","journal-title":"J. Periodontol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Razooqi, Z., H\u00f6glund \u00c5berg, C., Kwamin, F., Claesson, R., Haubek, D., Oscarsson, J., and Johansson, A. (2022). Aggregatibacter actinomycetemcomitans and Filifactor alocis as Associated with Periodontal Attachment Loss in a Cohort of Ghanaian Adolescents. Microorganisms, 10.","DOI":"10.3390\/microorganisms10122511"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"49","DOI":"10.2174\/1874210600802010049","article-title":"Optimizing qPCR for the Quantification of Periodontal Pathogens in a Complex Plaque Biofilm","volume":"2","author":"Kirakodu","year":"2008","journal-title":"Open Dent. J."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5375","DOI":"10.1128\/IAI.69.9.5375-5384.2001","article-title":"Structural and genetic analyses of O polysaccharide from Actinobacillus actinomycetemcomitans serotype f","volume":"69","author":"Kaplan","year":"2001","journal-title":"Infect. Immun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1034\/j.1600-051X.1999.260803.x","article-title":"Actinobacillus actinomycetemcomitans in Chinese adults: Serotype distribution and analysis of the leukotoxin gene promoter locus","volume":"26","author":"Mombelli","year":"1999","journal-title":"J. Clin. Periodontol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1034\/j.1399-302X.2003.00034.x","article-title":"Distribution of Actinobacillus actinomycetemcomitans serotypes and Porphyromonas gingivalis in Japanese adults","volume":"18","author":"Yoshida","year":"2003","journal-title":"Oral. Microbiol. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1128\/iai.40.1.205-212.1983","article-title":"Studies of leukotoxin from Actinobacillus actinomycetemcomitans using the promyelocytic HL-60 cell line","volume":"40","author":"Zambon","year":"1983","journal-title":"Infect. Immun."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/j.1399-302X.1991.tb00462.x","article-title":"Distribution of Actinobacillus actinomycetemcomitans serotypes in periodontal health and disease","volume":"6","author":"Asikainen","year":"1991","journal-title":"Oral. Microbiol. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/j.1600-0722.2004.00192.x","article-title":"Relationship of Actinobacillus actinomycetemcomitans serotypes to periodontal condition: Prevalence and proportions in subgingival plaque","volume":"113","author":"Yang","year":"2005","journal-title":"Eur. J. Oral Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1111\/j.1600-051X.2010.01649.x","article-title":"Prevalence and distribution of Aggregatibacter actinomycetemcomitans serotypes and the JP2 clone in a Greek population","volume":"38","author":"Sakellari","year":"2011","journal-title":"J. Clin. Periodontol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1111\/j.1600-051X.2008.01218.x","article-title":"Java project on periodontal diseases: Serotype distribution of Aggregatibacter actinomycetemcomitans and serotype dynamics over an 8-year period","volume":"35","year":"2008","journal-title":"J. Clin. Periodontol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1099\/13500872-140-8-2049","article-title":"Population structure of Actinobacillus actinomycetemcomitans: A framework for studies of disease-associated properties","volume":"140","author":"Poulsen","year":"1994","journal-title":"Microbiology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3037","DOI":"10.1128\/jcm.35.12.3037-3042.1997","article-title":"Racial tropism of a highly toxic clone of Actinobacillus actinomycetemcomitans associated with juvenile periodontitis","volume":"35","author":"Haubek","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1034\/j.1600-0447.2002.201238.x","article-title":"Actinobacillus actinomycetemcomitans proportion of subgingival bacterial flora in relation to its clonal type","volume":"110","author":"Lakio","year":"2002","journal-title":"Eur. J. Oral. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1399-302X.1993.tb00657.x","article-title":"Significance of serum antibody against surface antigens of Actinobacillus actinomycetemcomitans in patients with adult periodontitis","volume":"8","author":"Saito","year":"1993","journal-title":"Oral. Microbiol. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1111\/j.1600-0765.1996.tb01415.x","article-title":"Comparison of polymerase chain reaction and culture methods for detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in subgingival plaque samples","volume":"31","author":"Riggio","year":"1996","journal-title":"J. Periodontal Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1399-302X.1988.tb00596.x","article-title":"Associations between microbial species in subgingival plaque samples","volume":"3","author":"Socransky","year":"1988","journal-title":"Oral. Microbiol. Immunol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1600-051X.1998.tb02419.x","article-title":"Microbial complexes in subgingival plaque","volume":"25","author":"Socransky","year":"1998","journal-title":"J. Clin. Periodontol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"506","DOI":"10.1902\/jop.1989.60.9.506","article-title":"Actinobacillus actinomycetemcomitans serotypes and leukotoxicity in Korean localized juvenile periodontitis","volume":"60","author":"Chung","year":"1989","journal-title":"J. Periodontol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1034\/j.1399-302X.1999.140204.x","article-title":"Actinobacillus actinomycetemcomitans serotype e\u2014Biotypes, genetic diversity and distribution in relation to periodontal status","volume":"14","author":"Saarela","year":"1999","journal-title":"Oral. Microbiol. Immunol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3859","DOI":"10.1128\/JCM.00653-07","article-title":"Aggregatibacter actinomycetemcomitans and Its Relationship to Initiation of Localized Aggressive Periodontitis: Longitudinal Cohort Study of Initially Healthy Adolescents","volume":"45","author":"Fine","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1034\/j.1399-302X.2002.170301.x","article-title":"Actinobacillus actinomycetemcomitans in a rural adult population in southern Thailand","volume":"17","author":"Widar","year":"2002","journal-title":"Oral. Microbiol. Immunol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/8\/805\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:26:49Z","timestamp":1760034409000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/8\/805"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,13]]},"references-count":49,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2025,8]]}},"alternative-id":["pathogens14080805"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14080805","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,13]]}}}