{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:41:11Z","timestamp":1760060471707,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,24]],"date-time":"2025-08-24T00:00:00Z","timestamp":1755993600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Micro-organisms are abundant in nature and can also be found in hospital settings, causing high rates of infections. This study aimed to identify bacteria isolated from a veterinary hospital, as well as to perform antimicrobial susceptibility testing using the disk diffusion method (Kirby\u2013Bauer), biofilm production tests using 96-well polystyrene microtiter plates and crystal violet dye, and genetic analysis of the ica operon of Staphylococcus isolates. Three collections were made from eleven surfaces and objects in the hospital\u2019s non-critical areas (general areas) and critical areas (surgical center), totaling thirty-three samples. A total of 66 different bacterial isolates were obtained, with 77% (51\/66) Gram-positive and 23% (29\/66) Gram-negative. Resistance profiles were found for multidrug-resistance (MDR), methicillin-resistant Staphylococcus aureus (MRSA), methicillin-resistant Staphylococcus epidermidis (MRSE), and other unidentified species of methicillin-resistant coagulase-negative (MRCNS) and extended-spectrum beta-lactamase (ESBL), as well as biofilm production rates of 57% (38\/66) of the isolates. Analysis of the operon genes for Staphylococcus sp. showed divergence in some samples when compared to the phenotypic test performed. In summary, there is a high presence of micro-organisms with resistance and virulence factors spread throughout the various areas of the veterinary hospital.<\/jats:p>","DOI":"10.3390\/pathogens14090845","type":"journal-article","created":{"date-parts":[[2025,8,25]],"date-time":"2025-08-25T00:10:36Z","timestamp":1756080636000},"page":"845","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobial Resistance and Biofilm Formation in Bacterial Species Isolated from a Veterinary Hospital"],"prefix":"10.3390","volume":"14","author":[{"given":"Vanessa","family":"Bridi","sequence":"first","affiliation":[{"name":"Laboratory of Bacteriology and Micology, Biomedicine Course, Institute of Health Sciences, Jatob\u00e1 Campus, Federal University of Jata\u00ed, Jata\u00ed 75801-615, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00e9bora Pereira Gomes","family":"do Prado","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Micology, Biomedicine Course, Institute of Health Sciences, Jatob\u00e1 Campus, Federal University of Jata\u00ed, Jata\u00ed 75801-615, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"St\u00e9fanne Rodrigues Rezende","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Micology, Biomedicine Course, Institute of Health Sciences, Jatob\u00e1 Campus, Federal University of Jata\u00ed, Jata\u00ed 75801-615, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina Pedrosa","family":"Pedretti","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Micology, Biomedicine Course, Institute of Health Sciences, Jatob\u00e1 Campus, Federal University of Jata\u00ed, Jata\u00ed 75801-615, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmar Gon\u00e7alves Pereira","family":"Filho","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Micology, Biomedicine Course, Institute of Health Sciences, Jatob\u00e1 Campus, Federal University of Jata\u00ed, Jata\u00ed 75801-615, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9110-6350","authenticated-orcid":false,"given":"Wagner Gouv\u00eaa dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Genetics and Molecular Biology Laboratory, Biomedicine Course, Institute of Health Sciences, Jatob\u00e1 Campus, Federal University of Jata\u00ed, Jata\u00ed 75801-615, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5816-1427","authenticated-orcid":false,"given":"Hanstter Hallison Alves","family":"Rezende","sequence":"additional","affiliation":[{"name":"Laboratory of Bacteriology and Micology, Biomedicine Course, Institute of Health Sciences, Jatob\u00e1 Campus, Federal University of Jata\u00ed, Jata\u00ed 75801-615, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,24]]},"reference":[{"key":"ref_1","unstructured":"Brasil (1998). Portaria n\u00ba 2.616 de 12 de Maio de 1998. Disp\u00f5e Sobre as Diretrizes e Normas Para a Preven\u00e7\u00e3o e o Controle das Infec\u00e7\u00f5es Hospitalares, Minist\u00e9rio da Sa\u00fade."},{"key":"ref_2","first-page":"277","article-title":"Preven\u00e7\u00e3o e controle de infec\u00e7\u00f5es relacionadas a assist\u00eancia \u00e0 sa\u00fade: Fatores intr\u00ednsecos ao paciente","volume":"43","author":"Hayashi","year":"2017","journal-title":"HU Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.cvsm.2014.11.009","article-title":"Hospital-Associated Infections in Small Animal Practice","volume":"45","author":"Stull","year":"2015","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_4","unstructured":"Verdial, C.S.S. (2020). Preven\u00e7\u00e3o de Infec\u00e7\u00f5es Nasocomiais: Controle bacteriol\u00f3gico de superf\u00edcies hospitalares de unidade de isolamento e conten\u00e7\u00e3o biol\u00f3gica do hospital escolar da FMV-ULisboa. [Master\u2019s Thesis, Universidade de Lisboa]."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1128\/JCM.44.3.857-860.2006","article-title":"Isolation of a strictly anaerobic strain of Staphylococcus epidermidis","volume":"44","author":"Rowlinson","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1038\/s41579-021-00540-9","article-title":"Bacillus subtilis biofilm formation and social interactions","volume":"19","author":"Arnaouteli","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ciofu, O., and Tolker-Nielsen, T. (2019). Tolerance and resistance of Pseudomonas aeruginosa biofilms to antimicrobial agents-how P. aeruginosa can escape antibiotics. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.00913"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Chiba, A., Seki, M., Suzuki, Y., Kinjo, Y., Mizunoe, Y., and Sugimoto, S. (2022). Staphylococcus aureus utilizes environmental RNA as a building material in specific polysaccharide-dependent biofilms. NPJ Biofilms Microbiomes, 8.","DOI":"10.1038\/s41522-022-00278-z"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Yin, W., Wang, Y., Liu, L., and He, J. (2019). Biofilms: The microbial \u201cprotective clothing\u201d in extreme environments. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20143423"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Abebe, G.M. (2020). The role of bacterial biofilm in antibiotic resistance and food contamination. Int. J. Microbiol., 2020.","DOI":"10.1155\/2020\/1705814"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhao, A., Sun, J., and Liu, Y. (2023). Understanding bacterial biofilms: From definition to treatment strategies. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1137947"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Fran\u00e7ois, P., Schrenzel, J., and G\u00f6tz, F. (2023). Biology and Regulation of Staphylococcal Biofilm. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065218"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Cataneli Pereira, V., Pinheiro-Hubinger, L., de Oliveira, A., Moraes Riboli, D.F., Benini Martins, K., Calixto Romero, L., and Ribeiro de Souza da Cunha, M.D.L. (2020). Detection of the agr System and Resistance to Antimicrobials in Biofilm-Producing S. epidermidis. Molecules, 25.","DOI":"10.3390\/molecules25235715"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ruhal, R., and Kataria, R. (2021). Biofilm patterns in gram-positive and gram-negative bact\u00e9ria. Microbiol. Res., 251.","DOI":"10.1016\/j.micres.2021.126829"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"297","DOI":"10.5326\/JAAHA-MS-6903","article-title":"AAHA Infection Control, Prevention, and Biosecurity Guidelines","volume":"54","author":"Stull","year":"2018","journal-title":"J. Am. Anim. Hosp. Assoc."},{"key":"ref_16","unstructured":"Gaschen, F. (2008, January 24). Nosocomial Infection: Prevention and Approach. Proceedings of the World Small Animal Veterinary Association World Congress, Dublin, Ireland."},{"key":"ref_17","first-page":"E1","article-title":"Guidelines for reducing pathogens in veterinary hospitals: Disinfectant selection, cleaning protocols, and hand hygiene","volume":"32","author":"Portner","year":"2010","journal-title":"Compendium"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1080\/17415349.2018.1489320","article-title":"Hospital-acquired infections in the veterinary establishment","volume":"33","author":"Mann","year":"2018","journal-title":"Vet. Nurs. J."},{"key":"ref_19","unstructured":"Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria (Anvisa) (2013). Microbiologia Cl\u00ednica Para o Controle de Infec\u00e7\u00e3o Relacionada \u00e0 Assist\u00eancia a Sa\u00fade. M\u00f3dulo 6: Detec\u00e7\u00e3o e Identifica\u00e7\u00e3o de Bact\u00e9rias de Import\u00e2ncia M\u00e9dica, Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria."},{"key":"ref_20","unstructured":"Brazilian Committee on Antimicrobial Susceptibility Testing (BrCAST) (2023). Orienta\u00e7\u00f5es do EUCast\/BRCast para a detec\u00e7\u00e3o de mecanismos de resist\u00eancia e resist\u00eancias espec\u00edficas de import\u00e2ncia cl\u00ednica e\/ou epidemiol\u00f3gica, [2nd ed.]."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1111\/j.1600-0463.2007.apm_630.x","article-title":"Quantification of biofilm in microtiter plates: Overview of testing conditions and practical recommendations for assessment of biofilm production by Staphylococci","volume":"115","author":"Stepanovic","year":"2007","journal-title":"APMIS"},{"key":"ref_23","unstructured":"Olsvick, O., and Strockbine, N.A. (1993). PCR detection of heat-stable, heat-labile, and Shiga-like toxin genes in Escherichia coli. Diagnostic Molecular Microbiology: Principles and Applications, American Society for Microbiology."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1292\/jvms.15-0043","article-title":"Phenotypic and genotypic characterization of canine pyoderma isolates of Staphylococcus pseudintermedius for biofilm formation","volume":"77","author":"Proietti","year":"2015","journal-title":"J. Vet. Med. Sci."},{"key":"ref_25","unstructured":"Pereira, M.G. (1999). Epidemiologia: Teoria e Pr\u00e1tica, Guanabara Koogan."},{"key":"ref_26","first-page":"371","article-title":"Suspectibilidade antimicrobiana de bact\u00e9rias isoladas de colch\u00f5es hospitalares","volume":"49","author":"Mahl","year":"2017","journal-title":"Rev. Bras. An\u00e1lises Cl\u00ednicas"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e191724","DOI":"10.11606\/issn.1678-4456.bjvras.2022.191724","article-title":"Detection of beta-lactamase-producing Enterobacteriaceae in a veterinary hospital environment","volume":"59","author":"Arrais","year":"2022","journal-title":"Braz. J. Vet. Res. Anim. Sci."},{"key":"ref_28","first-page":"2236","article-title":"Perfil de resist\u00eancia em bact\u00e9rias isoladas de superf\u00edcies de um hospital p\u00fablico de Juazeiro do Norte-CE","volume":"44","author":"Silva","year":"2018","journal-title":"Rev. Sa\u00fade (Sta. Maria)"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"40","DOI":"10.17058\/reci.v3i2.3290","article-title":"Microrganismos isolados de superf\u00edcies da UTI adulta em um hospital do Vale do Rio Pardo\u2014R.S","volume":"3","author":"Renner","year":"2013","journal-title":"Rev. Epidemiol. Controle Infec\u00e7\u00e3o"},{"key":"ref_30","first-page":"e06706","article-title":"Detection of the main multiresistant microorganisms in the environment of a teaching veterinary hospital in Brazil","volume":"41","author":"Sfaciotte","year":"2021","journal-title":"Braz. J. Vet. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"835","DOI":"10.1089\/mdr.2019.0305","article-title":"Bacteria with a Potential for Multidrug Resistance in Hospital Material","volume":"27","author":"Souza","year":"2020","journal-title":"Microb. Drug Resist."},{"key":"ref_32","unstructured":"Ag\u00eancia Nacional de Vigil\u00e2ncia Sanit\u00e1ria (ANVISA) (2024, March 10). Caderno 2\u2014Crit\u00e9rios Diagn\u00f3sticos de Infec\u00e7\u00e3o Relacionada \u00e0 Assist\u00eancia \u00e0 Sa\u00fade, Available online: https:\/\/antigo.anvisa.gov.br\/resultado-de-busca?_3_advancedSearch=false&_3_andOperator=true&_3_assetCateryIds=508782&_3_cur=1&_3_delta=20&_3_formDate=1441824476958&_3_format=&_3_groupId=0&_3_keywords=Caderno+2+-+Crit%C3%A9rios+Diagn%C3%B3sticos+de+Infec%C3%A7%C3%A3o+Relacionada+\u00e0+Assist%C3%AAncia+\u00e0+Sa%C3%BAde&_3_resetCur=false&_3_struts_action=%2Fsearch%2Fsearch."},{"key":"ref_33","first-page":"51938","article-title":"An\u00e1lise microbiol\u00f3gica de bioaeross\u00f3is em uma unidade de tratamento intensivo de pacientes afetados por COVID-19","volume":"11","year":"2021","journal-title":"J. Res. Dev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/nrmicro.2016.94","article-title":"Biofilms: An emergent form of bacterial life","volume":"14","author":"Flemming","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_35","unstructured":"Damasceno, Q.S. (2010). Caracter\u00edsticas Epidemiol\u00f3gicas dos Microrganismos Resistentes Presentes em Reservat\u00f3rios de uma Unidade de Terapia Intensiva. [Disserta\u00e7\u00e3o de Mestrado, Universidade Federal de Minas Gerais, Escola de Enfermagem]."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1136\/vr.100508","article-title":"Recovery of Staphylococci from computer keyboards in a veterinary medical centre and the effect of routine cleaning","volume":"170","author":"Bender","year":"2012","journal-title":"Vet. Rec."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1590\/1982-0194201500027","article-title":"Equipment contamination in an intensive care unit","volume":"8","author":"Cordeiro","year":"2015","journal-title":"Acta Paul. Enferm."},{"key":"ref_38","first-page":"e74774","article-title":"Bact\u00e9rias resistentes isoladas de superf\u00edcies inanimadas em um hospital p\u00fablico","volume":"26","author":"Machado","year":"2021","journal-title":"Cogitare Enferm."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Shoen, H.R.C., Rose, S., Ramsey, A.S., Morais, H., and Bermudez, L.E. (2019). Analysis of staphylococcus infections in a veterinary teaching hospital from 2012 to 2015. Comp. Immunol. Microbiol. Infect. Dis., 66.","DOI":"10.1016\/j.cimid.2019.101332"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/j.1532-950X.2014.12267.x","article-title":"Prospective surgical site infection surveillance in dogs","volume":"44","author":"Turk","year":"2015","journal-title":"Vet. Surg."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Ghosh, A., Kukanich, K., Brown, C.E., and Zurek, L. (2012). Resident Cats in Small Animal Veterinary Hospitals Carry Multi-Drug Resistant Enterococci and are Likely Involved in Cross Contamination of the Hospital Environment. Front. Microbiol., 3.","DOI":"10.3389\/fmicb.2012.00062"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1016\/j.ajic.2016.11.005","article-title":"Are Hospital floors na underappreciated reservoir for transmission of health care-associated pathogens?","volume":"45","author":"Deshpande","year":"2017","journal-title":"Am. J. Infect. Control."},{"key":"ref_43","first-page":"193","article-title":"Antimicrobial Susceptibility and Distribution of Multidrug-Resistant Organisms Isolated From Environmental Surfaces and Hands of Healthcare Workers in a Small Animal Hospital","volume":"66","author":"Oh","year":"2018","journal-title":"Jpn. J. Vet. Res."},{"key":"ref_44","first-page":"175","article-title":"Enterobact\u00e9rias produtoras de carbapenemase: Preven\u00e7\u00e3o da dissemina\u00e7\u00e3o de superbact\u00e9rias em UTI\u2019s","volume":"14","author":"Paula","year":"2016","journal-title":"Ci\u00eancias Sa\u00fade"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1590\/S0037-86822010000400019","article-title":"Enterobact\u00e9rias produtoras de ESBL em Passo fundo, Estado do Rio Grande do Sul, Brasil","volume":"43","author":"Lago","year":"2010","journal-title":"Soc. Bras. Med. Trop."},{"key":"ref_46","first-page":"96","article-title":"Perfil da Infec\u00e7\u00e3o Hospitalar em um Hospital Universit\u00e1rio","volume":"17","author":"Nogueira","year":"2009","journal-title":"Rev. Enferm. UERJ"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e20101421509","DOI":"10.33448\/rsd-v10i14.21509","article-title":"Avalia\u00e7\u00e3o da popula\u00e7\u00e3o bacteriana na superf\u00edcie de macas de um hospital universit\u00e1rio de Pernambuco","volume":"10","author":"Souza","year":"2021","journal-title":"Res. Soc. Dev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.jgar.2021.08.001","article-title":"Environmental antimicrobial resistance and its drivers: A potential threat to public health","volume":"27","author":"Samreen","year":"2021","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e316","DOI":"10.1016\/S2542-5196(17)30141-9","article-title":"Restricting the use of antibiotics in food-producing animals and its associations with antibiotic resistance in food-producing animals and human beings: A systematic review and meta-analysis","volume":"1","author":"Tang","year":"2017","journal-title":"Lancet Planet. Health"},{"key":"ref_50","first-page":"186","article-title":"Estetosc\u00f3pios de uso hospitalar como fonte de infec\u00e7\u00e3o por bact\u00e9rias resistentes produtoras de biofilme","volume":"55","author":"Silva","year":"2023","journal-title":"Rev. Bras. An\u00e1lises Cl\u00ednicas"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1099\/jmm.0.46799-0","article-title":"Formation and properties of in vitro biofilms of ica-negative Staphylococcus epidermidis clinical isolates","volume":"56","author":"Qin","year":"2007","journal-title":"J. Med. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1038\/nrmicro2182","article-title":"Staphylococcus epidermidis\u2014The \u201caccidental\u201d pathogen","volume":"7","author":"Otto","year":"2009","journal-title":"Nat. Rev. Microbiol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/9\/845\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:35:02Z","timestamp":1760034902000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/9\/845"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,24]]},"references-count":52,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["pathogens14090845"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14090845","relation":{},"ISSN":["2076-0817"],"issn-type":[{"type":"electronic","value":"2076-0817"}],"subject":[],"published":{"date-parts":[[2025,8,24]]}}}