{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T08:57:04Z","timestamp":1773392224243,"version":"3.50.1"},"reference-count":57,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T00:00:00Z","timestamp":1756425600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004564","name":"Ministry of Education, Science, and Technological Development of the Republic of Serbia","doi-asserted-by":"publisher","award":["451-03-136\/2025-03\/200003"],"award-info":[{"award-number":["451-03-136\/2025-03\/200003"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004564","name":"Ministry of Education, Science, and Technological Development of the Republic of Serbia","doi-asserted-by":"publisher","award":["451-03-137\/2025-03\/200113"],"award-info":[{"award-number":["451-03-137\/2025-03\/200113"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The increased incidence of periodontitis, the resistance of periodontal pathogens to antibiotics, and the adverse effects of certain drugs used in general dentistry present a strong rationale for seeking safe and effective plant-based treatments for periodontitis. HPLC-DAD analysis of a commercial herbal tincture confirmed the presence of rosmarinic acid (1102.79 \u00b1 21.56 \u00b5g\/mL), luteolin-7-O-glucoside (358.06 \u00b1 5.64 \u00b5g\/mL), and isorhamnetin (24.17 \u00b1 0.49 \u00b5g\/mL), bioactive phytochemicals known for their antimicrobial and anti-inflammatoryproperties. The randomized prospective study analyzed Tinctura paradentoica\u00ae as an adjunct to anti-infectious non-surgical periodontal therapy (NSPT) on clinical and microbiological parameters in patients with moderate periodontitis (Stage II, Grade A). All 60 recruited participants were randomly allocated to either the intervention group (NSPT + Tinctura paradentoica\u00ae) or the control group (NSPT alone). The rate of prevalence of the following periodontopathogenic microorganisms (Treponema denticola, Tannerella forsythensis), assessed by polymerase chain reaction (PCR) analysis, was significantly lower in the intervention group (p &lt; 0.001), but no statistically significant difference was found for Porphyromonas gingivalis. The herbal tincture, combined with NSPT, produces a short-term reduction in periodontal clinical parameters (Green\u2013Vermilion plaque index, bleeding on probing index (BOP), and clinical attachment level (CAL), without clinical relevance, and the prevalence of the following bacteria species (Tannerella forsythensis, Treponema denticola).<\/jats:p>","DOI":"10.3390\/pathogens14090861","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T16:42:21Z","timestamp":1756485741000},"page":"861","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Microbiological and Clinical Short-Term Evaluation of the Efficacy of an Herbal Tincture as an Adjunctive Treatment in the Management of Stage II, Grade A Periodontitis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6564-5131","authenticated-orcid":false,"given":"Milica","family":"Petrovi\u0107","sequence":"first","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Dental Clinic, Faculty of Medicine, University of Ni\u0161, 18108 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ljiljana","family":"Kesi\u0107","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Dental Clinic, Faculty of Medicine, University of Ni\u0161, 18108 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2880-6519","authenticated-orcid":false,"given":"Bojana","family":"Miladinovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Ni\u0161, 18108 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radmila","family":"Obradovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Dental Clinic, Faculty of Medicine, University of Ni\u0161, 18108 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Pej\u010di\u0107","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Dental Clinic, Faculty of Medicine, University of Ni\u0161, 18108 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4586-5115","authenticated-orcid":false,"given":"Marija","family":"Bojovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Dental Clinic, Faculty of Medicine, University of Ni\u0161, 18108 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2086-9593","authenticated-orcid":false,"given":"Katarina","family":"\u0160avikin","sequence":"additional","affiliation":[{"name":"Institute for Medicinal Plants Research \u201cDr. Josif Pan\u010di\u0107\u201d, Tadeu\u0161a Ko\u0161\u0107u\u0161ka 1, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9684-2637","authenticated-orcid":false,"given":"Jelena","family":"\u017divkovi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Medicinal Plants Research \u201cDr. Josif Pan\u010di\u0107\u201d, Tadeu\u0161a Ko\u0161\u0107u\u0161ka 1, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Stankovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Dental Clinic, Faculty of Medicine, University of Ni\u0161, 18108 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8551-274X","authenticated-orcid":false,"given":"Du\u0161anka","family":"Kiti\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Faculty of Medicine, University of Ni\u0161, 18108 Ni\u0161, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hashim, N.T., Babiker, R., Chaitanya, N.C.S.K., Mohammed, R., Priya, S.P., Padmanabhan, V., Ahmed, A., Dasnadi, S.P., Islam, M.S., and Gismalla, B.G. 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