{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T01:55:32Z","timestamp":1793325332759,"version":"4.5.0"},"reference-count":33,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T00:00:00Z","timestamp":1757721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UB Research Nexus","award":["CNFIS-FDI-2025-F-0364"],"award-info":[{"award-number":["CNFIS-FDI-2025-F-0364"]}]},{"name":"UB Research Nexus","award":["8 (I8)-contract CF 68"],"award-info":[{"award-number":["8 (I8)-contract CF 68"]}]},{"name":"UB Research Nexus","award":["23020101"],"award-info":[{"award-number":["23020101"]}]},{"name":"Ministry of Research, Innovation and Digitalization through the National Recovery and Resilience Plan (PNRR) of Romania, Pillar III","award":["CNFIS-FDI-2025-F-0364"],"award-info":[{"award-number":["CNFIS-FDI-2025-F-0364"]}]},{"name":"Ministry of Research, Innovation and Digitalization through the National Recovery and Resilience Plan (PNRR) of Romania, Pillar III","award":["8 (I8)-contract CF 68"],"award-info":[{"award-number":["8 (I8)-contract CF 68"]}]},{"name":"Ministry of Research, Innovation and Digitalization through the National Recovery and Resilience Plan (PNRR) of Romania, Pillar III","award":["23020101"],"award-info":[{"award-number":["23020101"]}]},{"name":"Ministry of Research, Innovation and Digitalization (MCID)","award":["CNFIS-FDI-2025-F-0364"],"award-info":[{"award-number":["CNFIS-FDI-2025-F-0364"]}]},{"name":"Ministry of Research, Innovation and Digitalization (MCID)","award":["8 (I8)-contract CF 68"],"award-info":[{"award-number":["8 (I8)-contract CF 68"]}]},{"name":"Ministry of Research, Innovation and Digitalization (MCID)","award":["23020101"],"award-info":[{"award-number":["23020101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: Antimicrobial resistance (AMR) is a major public health concern. Urinary tract infections (UTIs) account for up to 85\u201390% of community-acquired cases. The COVID-19 pandemic disrupted healthcare access and may have influenced resistance patterns. In this context, we retrospectively evaluated the antibiotic resistance dynamics of various bacterial strains isolated between 2019 and 2023 in a hospital unit; Methods: A total of 8217 clinical specimens (urine, wound secretions, sputum, pharyngeal exudate, nasal exudate, tracheal secretions, vaginal and cervical secretions, puncture fluids, purulent secretions, blood, ear secretions, eye secretions) were processed using standard microbiological techniques. Pathogen identification and susceptibility testing were performed with the VITEK 2 Compact system, following CLSI guidelines. Results: Following the analysis of 8217 clinical samples collected over a five-year period (2019\u20132023), a total of 2900 microorganisms were isolated and identified. Among these, the most frequently encountered were E. coli strains, with 1204 isolates. Urine cultures represented 71.3% of all processed samples. Out of these 5860 urine cultures, 1530 (26%) were positive. The resistance of E. coli strains to ampicillin (48\u201355.2%), trimethoprim\/sulfamethoxazole (22.9\u201334%), and ciprofloxacin (21.4\u201331.5%) remained high throughout the period. ESBL-producing strains peaked at 17.6% in 2020, with multidrug resistance rates ranging from 14% to 22.4%. Conclusions: E. coli strains displayed persistently high resistance to ampicillin, trimethoprim\/sulfamethoxazole, and ciprofloxacin, with peaks in ESBL production and multidrug resistance during the COVID-19 pandemic. These trends underscore the importance of continuous surveillance and antibiotic stewardship, with direct implications for empirical UTI therapy and broader strategies to mitigate the public health impact of antimicrobial resistance.<\/jats:p>","DOI":"10.3390\/pathogens14090927","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T08:56:46Z","timestamp":1758013006000},"page":"927","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Trends in Antibiotic Resistance of Escherichia coli Strains Isolated from Clinical Samples (2019\u20132023): A Hospital-Based Retrospective Analysis"],"prefix":"10.3390","volume":"14","author":[{"given":"Claudia Daniela","family":"Goleanu (Vasiloiu)","sequence":"first","affiliation":[{"name":"Microbiology-Immunology Department, Faculty of Biology, University of Bucharest, 050095 Bucharest, Romania"},{"name":"Department of Laboratory Medicine, \u201cProf. Dr. Constantin Angelescu\u201d Hospital, Aleea C\u0103uza\u0219i 49-51, 030167 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2409-0966","authenticated-orcid":false,"given":"Corneliu Ovidiu","family":"Vrancianu","sequence":"additional","affiliation":[{"name":"Doctoral School, Carol Davila University of Medicine and Pharmacy, Eroii Sanitari 8, District 5, 050474 Bucharest, Romania"},{"name":"The Research Institute of the University of Bucharest\u2014ICUB, \u0218oseaua Panduri 90, District 5, 050663 Bucharest, Romania"},{"name":"National Institute of Research and Development for Biological Sciences, 296 Splaiul Independentei, District 6, 060031 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria Adelina","family":"Goleanu","sequence":"additional","affiliation":[{"name":"Department of General Medicine, Carol Davila University of Medicine and Pharmacy, Eroii Sanitari 8, District 5, 050474 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica Marilena","family":"Tantu","sequence":"additional","affiliation":[{"name":"Department of Medical Assistance and Physical Therapy, Pitesti University Center, T\u00e2rgu din Vale 1, 110040 Pite\u0219ti, Romania"},{"name":"Faculty of Science, Physical Education and Informatics, National University of Science and Technology, Politehnica, Splaiul Independen\u021bei 313, District 6, 060042 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ortansa","family":"Csutak","sequence":"additional","affiliation":[{"name":"Department of Genetics, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 060101 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5501","DOI":"10.2147\/IDR.S425728","article-title":"Trends in Antibiotic Resistance Patterns and Burden of Escherichia Coli Infections in Young Children: A Retrospective Cross-Sectional Study in Shenzhen, China from 2014\u20132018","volume":"16","author":"Patil","year":"2023","journal-title":"Infect. 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