{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T08:26:38Z","timestamp":1787905598187,"version":"build-2784847793"},"reference-count":59,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007086","name":"Sel\u00e7uk University Scientific Research Projects Coordinator","doi-asserted-by":"publisher","award":["22401140"],"award-info":[{"award-number":["22401140"]}],"id":[{"id":"10.13039\/501100007086","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Colorectal cancer (CRC) is one of the most common cancers worldwide and a significant global health issue. The human gut microbiota, a complex ecosystem hosting numerous microorganisms such as bacteria, viruses, fungi, and protozoa, plays a crucial role. Increasing evidence indicates that gut microbiota is involved in CRC pathogenesis. In this study, the gut microbiota profiles, short-chain fatty acids, zonulin, and lipopolysaccharide-binding protein levels of newly diagnosed CRC patients were analyzed along with healthy controls to elucidate the relationship between CRC and the gut microbiota. The study included 16 newly diagnosed CRC patients and 16 healthy individuals. For microbiota analysis, DNA isolation from stool samples was performed using the Quick-DNA\u2122 Fecal\/Soil Microbe Miniprep Kit followed by sequencing using the MinION device. Data processing was conducted using Guppy software (version 6.5.7) and the Python (3.12) programming language. ELISA kits from Elabscience were utilized for analyzing LBP and zonulin serum levels. Fecal short-chain fatty acids were analyzed using GC-MS\/MS equipped with a flame ionization detector and DB-FFAP column. Microbial alpha diversity, assessed using Shannon and Simpson indices, was found to be lower in CRC patients compared to healthy controls (p = 0.045, 0.017). Significant differences in microbial beta diversity were observed between the two groups (p = 0.004). At the phylum level, Bacteroidota was found to be decreased in CRC patients (p = 0.027). Potential biomarker candidates identified included Enterococcus faecium, Ruminococcus bicirculans, Enterococcus gilvus, Enterococcus casseliflavus, Segatella oris, and Akkermansia muciniphila. Serum zonulin levels were higher in CRC patients (CRC = 70.1 \u00b1 26.14, Control = 53.93 \u00b1 17.33, p = 0.048). There is a significant relationship between gut microbiota and CRC. A multifactorial evaluation of this relationship could shed light on potential biomarker identification and the development of new treatment options for CRC.<\/jats:p>","DOI":"10.3390\/pathogens14090953","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T13:04:44Z","timestamp":1758546284000},"page":"953","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Investigation of Intestinal Microbiota and Short-Chain Fatty Acids in Colorectal Cancer and Detection of Biomarkers"],"prefix":"10.3390","volume":"14","author":[{"given":"Esra","family":"Saylam","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Sel\u00e7uk University, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zben","family":"\u00d6zden","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology, Institute of Health Sciences, Ac\u0131badem University, Istanbul 34752, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma H\u00fcmeyra","family":"Yerlikaya","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, Sel\u00e7uk University, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah","family":"Sivrikaya","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine, Sel\u00e7uk University, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serdar","family":"Yormaz","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Faculty of Medicine, Sel\u00e7uk University, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U\u011fur","family":"Arslan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Sel\u00e7uk University, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Topkafa","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Processing Technology, Konya Technical University, Konya 42250, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salih","family":"Ma\u00e7in","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine, Sel\u00e7uk University, Konya 42130, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"ref_1","unstructured":"Globacan (2025, September 15). WHO. Available online: https:\/\/gco.iarc.fr\/en."},{"key":"ref_2","first-page":"209","article-title":"Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_3","first-page":"1","article-title":"Early detection for colorectal cancer: ASCO resource-stratified guideline","volume":"5","author":"Lopes","year":"2019","journal-title":"J. Glob. Oncol."},{"key":"ref_4","unstructured":"HSGM (2021). T\u00fcrkiye Cancer Control Program, Ministry of Health."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1152\/physrev.00045.2009","article-title":"Gut microbiota in health and disease","volume":"90","author":"Sekirov","year":"2010","journal-title":"Physiol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Das, B., and Nair, G.B. (2019). Homeostasis and dysbiosis of the gut microbiome in health and disease. J. Biosci., 44.","DOI":"10.1007\/s12038-019-9926-y"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1038\/s41392-022-00974-4","article-title":"Microbiota in health and diseases","volume":"7","author":"Hou","year":"2022","journal-title":"Signal Transduct. Target. Ther."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1111\/apt.13248","article-title":"dietary fibre\u2013microbiota interactions","volume":"42","author":"Simpson","year":"2015","journal-title":"Aliment. Pharmacol. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1089\/biores.2012.0223","article-title":"Butyrate histone deacetylase inhibitors","volume":"1","author":"Steliou","year":"2012","journal-title":"BioResearch Open Access"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00394-017-1445-8","article-title":"Gut microbiota functions: Metabolism of nutrients and other food components","volume":"57","author":"Rowland","year":"2018","journal-title":"Eur. J. Nutr."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3611","DOI":"10.1038\/ncomms4611","article-title":"The short-chain fatty acid acetate reduces appetite via a central homeostatic mechanism","volume":"5","author":"Frost","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/s13073-016-0303-2","article-title":"Impact of the gut microbiota on inflammation, obesity, and metabolic disease","volume":"8","author":"Neves","year":"2016","journal-title":"Genome Med."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Veres-Sz\u00e9kely, A., Sz\u00e1sz, C., Pap, D., Szebeni, B., Bokrossy, P., and Vannay, \u00c1. (2023). Zonulin as a Potential Therapeutic Target in Microbiota-Gut-Brain Axis Disorders: Encouraging Results and Emerging Questions. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24087548"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"69","DOI":"10.12688\/f1000research.20510.1","article-title":"All disease begins in the (leaky) gut: Role of zonulin-mediated gut permeability in the pathogenesis of some chronic inflammatory diseases","volume":"9","author":"Fasano","year":"2020","journal-title":"F1000Research"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Mignini, I., Piccirilli, G., Galasso, L., Termite, F., Esposto, G., Ainora, M.E., Gasbarrini, A., and Zocco, M.A. (2024). From the Colon to the Liver: How Gut Microbiota May Influence Colorectal Cancer Metastatic Potential. J. Clin. Med., 13.","DOI":"10.3390\/jcm13020420"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Gao, Z., Guo, B., Gao, R., Zhu, Q., and Qin, H. (2015). Microbiota disbiosis is associated with colorectal cancer. Front. Microbiol., 6.","DOI":"10.3389\/fmicb.2015.00020"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"7046","DOI":"10.1080\/21655979.2021.1972077","article-title":"The gut microbial diversity of colon cancer patients and the clinical significance","volume":"12","author":"He","year":"2021","journal-title":"Bioengineered"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e00630-18","DOI":"10.1128\/mBio.00630-18","article-title":"Leveraging existing 16S rRNA gene surveys to identify reproducible biomarkers in individuals with colorectal tumors","volume":"9","author":"Sze","year":"2018","journal-title":"MBio"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"96","DOI":"10.4103\/0973-1482.341139","article-title":"A metagenomic assessment of gut microbiota in Indian colon cancer patients","volume":"18","author":"Bamola","year":"2022","journal-title":"J. Cancer Res. Ther."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1038\/ismej.2011.109","article-title":"Structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers","volume":"6","author":"Wang","year":"2012","journal-title":"ISME J."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Zhao, L., Cho, W.C., and Nicolls, M.R. (2021). Colorectal cancer-associated microbiome patterns and signatures. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.787176"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Sasaki, K., Inoue, J., Sasaki, D., Hoshi, N., Shirai, T., Fukuda, I., Azuma, T., Kondo, A., and Osawa, R. (2019). Construction of a model culture system of human colonic microbiota to detect decreased Lachnospiraceae abundance and butyrogenesis in the feces of ulcerative colitis patients. Biotechnol. J., 14.","DOI":"10.1002\/biot.201800555"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Sagheddu, V., Patrone, V., Miragoli, F., Puglisi, E., and Morelli, L. (2016). Infant early gut colonization by Lachnospiraceae: High frequency of Ruminococcus gnavus. Front. Pediatr., 4.","DOI":"10.3389\/fped.2016.00057"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Khodaverdi, N., Zeighami, H., Jalilvand, A., Haghi, F., and Hesami, N. (2021). High frequency of enterotoxigenic Bacteroides fragilis and Enterococcus faecalis in the paraffin-embedded tissues of Iranian colorectal cancer patients. BMC Cancer, 21.","DOI":"10.1186\/s12885-021-09110-x"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Koliarakis, I., Messaritakis, I., Nikolouzakis, T.K., Hamilos, G., Souglakos, J., and Tsiaoussis, J. (2019). Oral bacteria and intestinal dysbiosis in colorectal cancer. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20174146"},{"key":"ref_26","first-page":"e22156","article-title":"The association of microbiome dysbiosis with colorectal cancer","volume":"14","author":"Artemev","year":"2022","journal-title":"Cureus"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1875796","DOI":"10.1080\/19490976.2021.1875796","article-title":"Blautia\u2014A new functional genus with potential probiotic properties?","volume":"13","author":"Liu","year":"2021","journal-title":"Gut Microbes"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Nie, K., Ma, K., Luo, W., Shen, Z., Yang, Z., Xiao, M., Tong, T., Yang, Y., and Wang, X. (2021). Roseburia intestinalis: A beneficial gut organism from the discoveries in genus and species. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.757718"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Weir, T.L., Manter, D.K., Sheflin, A.M., Barnett, B.A., Heuberger, A.L., and Ryan, E.P. (2013). Stool microbiome and metabolome differences between colorectal cancer patients and healthy adults. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0070803"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Cheng, Y., Ling, Z., and Li, L. (2020). The intestinal microbiota and colorectal cancer. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.615056"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2879","DOI":"10.1111\/1462-2920.12217","article-title":"Complete genome of a new Firmicutes species belonging to the dominant human colonic microbiota (\u2018Ruminococcus bicirculans\u2019) reveals two chromosomes and a selective capacity to utilize plant glucans","volume":"16","author":"Wegmann","year":"2014","journal-title":"Environ. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1756284818783606","DOI":"10.1177\/1756284818783606","article-title":"The controversial role of Enterococcus faecalis in colorectal cancer","volume":"11","author":"Taddei","year":"2018","journal-title":"Ther. Adv. Gastroenterol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Hanchi, H., Mottawea, W., Sebei, K., and Hammami, R. (2018). The genus Enterococcus: Between probiotic potential and safety concerns\u2014An update. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01791"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s00217-008-0927-6","article-title":"Probiotic Enterococcus faecium CRL 183 inhibit chemically induced colon cancer in male Wistar rats","volume":"228","author":"Sivieri","year":"2008","journal-title":"Eur. Food Res. Technol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e02612-22","DOI":"10.1128\/spectrum.02612-22","article-title":"Altered gut microbial profile accompanied by abnormal fatty acid metabolism activity exacerbates endometrial cancer progression","volume":"10","author":"Zhao","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Li, X., Yi, Y., Wu, T., Chen, N., Gu, X., Xiang, L., Jiang, Z., Li, J., and Jin, H. (2023). Integrated microbiome and metabolome analysis reveals the interaction between intestinal flora and serum metabolites as potential biomarkers in hepatocellular carcinoma patients. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1170748"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"17822","DOI":"10.1002\/cam4.6414","article-title":"Aging characteristics of colorectal cancer based on gut microbiota","volume":"12","author":"Wu","year":"2023","journal-title":"Cancer Med."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Zhang, F., Wang, J.L., Zhu, J., Si, S., Guo, H., Yue, X., and Wen, W. (2025). When a sore throat turns into deadly multiple serous cavity effusions: The role of Prevotella oris in rapidly progressing infection\u2014A case report. Front. Med., 12.","DOI":"10.3389\/fmed.2025.1517389"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Parker, B.J., Wearsch, P.A., Veloo, A.C., and Rodriguez-Palacios, A. (2020). The genus Alistipes: Gut bacteria with emerging implications to inflammation, cancer, and mental health. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.00906"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/j.chom.2016.03.007","article-title":"Lipocalin 2 protects from inflammation and tumorigenesis associated with gut microbiota alterations","volume":"19","author":"Moschen","year":"2016","journal-title":"Cell Host Microbe"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Baxter, N.T., Zackular, J.P., Chen, G.Y., and Schloss, P.D. (2014). Structure of the gut microbiome following colonization with human feces determines colonic tumor burden. Microbiome, 2.","DOI":"10.1186\/2049-2618-2-20"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"124","DOI":"10.7150\/jca.63578","article-title":"Akkermansia muciniphila administration exacerbated the development of colitis-associated colorectal cancer in mice","volume":"13","author":"Wang","year":"2022","journal-title":"J. Cancer"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2841","DOI":"10.1097\/CM9.0000000000001829","article-title":"Akkermansia muciniphila in inflammatory bowel disease and colorectal cancer","volume":"134","author":"Gu","year":"2021","journal-title":"Chin. Med. J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1388","DOI":"10.1093\/carcin\/bgv120","article-title":"Akkermansia muciniphila and Helicobacter typhlonius modulate intestinal tumor development in mice","volume":"36","author":"Dingemanse","year":"2015","journal-title":"Carcinogenesis"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Buc, E., Dubois, D., Sauvanet, P., Raisch, J., Delmas, J., Darfeuille-Michaud, A., Pezet, D., and Bonnet, R. (2013). High prevalence of mucosa-associated E. coli producing cyclomodulin and genotoxin in colon cancer. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0056964"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1158\/1078-0432.CCR-13-1343","article-title":"Colonization of the human gut by E. coli and colorectal cancer risk","volume":"20","author":"Bonnet","year":"2014","journal-title":"Clin. Cancer Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2185028","DOI":"10.1080\/19490976.2023.2185028","article-title":"Intestinal bacteria and colorectal cancer: Etiology and treatment","volume":"15","author":"Dougherty","year":"2023","journal-title":"Gut Microbes"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Serek, P., and Oleksy-Wawrzyniak, M. (2021). The effect of bacterial infections, probiotics and zonulin on intestinal barrier integrity. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222111359"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1007\/s10517-022-05552-w","article-title":"Blood Serum Zonulin in Colorectal Cancer, Autoimmune Bowel Diseases, and Irritable Bowel Syndrome","volume":"173","author":"Kushlinskii","year":"2022","journal-title":"Bull. Exp. Biol. Med."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"117","DOI":"10.3945\/ajcn.112.040949","article-title":"The effects of perioperative probiotic treatment on serum zonulin concentration and subsequent postoperative infectious complications after colorectal cancer surgery: A double-center and double-blind randomized clinical trial","volume":"97","author":"Liu","year":"2013","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_51","first-page":"115","article-title":"Plasma lipopolysaccharide-binding protein and colorectal cancer risk: A nested case\u2013control study in the multiethnic cohort","volume":"29","author":"Citronberg","year":"2018","journal-title":"Cancer Causes Control"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Fuke, N., Yamashita, T., Shimizu, S., Matsumoto, M., Sawada, K., Jung, S., Tokuda, I., Misawa, M., Suzuki, S., and Ushida, Y. (2023). Association of Plasma Lipopolysaccharide-Binding Protein Concentration with Dietary Factors, Gut Microbiota, and Health Status in the Japanese General Adult Population: A Cross-Sectional Study. Metabolites, 13.","DOI":"10.3390\/metabo13020250"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2205563","DOI":"10.1002\/advs.202205563","article-title":"Role of the Gut Microbiota and Its Metabolites in Tumorigenesis or Development of Colorectal Cancer","volume":"10","author":"Qu","year":"2023","journal-title":"Adv. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/s41396-021-01119-1","article-title":"Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer","volume":"16","author":"Ryu","year":"2022","journal-title":"ISME J."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1007\/s10620-018-5417-5","article-title":"Branched short-chain fatty acid isovaleric acid causes colonic smooth muscle relaxation via cAMP\/PKA pathway","volume":"64","author":"Blakeney","year":"2019","journal-title":"Dig. Dis. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3900","DOI":"10.1111\/cas.15920","article-title":"Isobutyric acid promotes colorectal cancer metastasis through activating RACK1","volume":"114","author":"Chen","year":"2023","journal-title":"Cancer Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.3945\/ajcn.112.046607","article-title":"Decreased dietary fiber intake and structural alteration of gut microbiota in patients with advanced colorectal adenoma","volume":"97","author":"Chen","year":"2013","journal-title":"Am. Clin. Nutr."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Mirzaei, R., Afaghi, A., Babakhani, S., Sohrabi, M.R., Hosseini-Fard, S.R., Babolhavaeji, K., Akbari, S.K.A., Yousefimashouf, R., and Karampoor, S. (2021). Role of microbiota-derived short-chain fatty acids in cancer development and prevention. Biomed. Pharmacother., 139.","DOI":"10.1016\/j.biopha.2021.111619"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5543","DOI":"10.3748\/wjg.v25.i36.5543","article-title":"Evaluation and comparison of short chain fatty acids composition in gut diseases","volume":"25","author":"Niccolai","year":"2019","journal-title":"World J. Gastroenterol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/9\/953\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:46:52Z","timestamp":1760035612000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/9\/953"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,22]]},"references-count":59,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["pathogens14090953"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14090953","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,22]]}}}