{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T23:51:14Z","timestamp":1785282674615,"version":"3.55.0"},"reference-count":45,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T00:00:00Z","timestamp":1758758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>(1) Background: Fungal infections of amniotic fluid, especially those caused by Candida spp., are rare but clinically important, as they can be correlated with preterm birth and poor neonatal outcomes. The aim of this study was to assess the antifungal susceptibility of Candida spp. isolated from amniotic fluid using an MIC (minimum inhibitory concentration)-based assay. (2) Methods: Forty consecutive, exploratory Candida isolates were identified from amniotic fluid samples at the \u201cCuza Vod\u0103\u201d Clinical Hospital of Obstetrics and Gynecology, Ia\u0219i, and were analyzed successively using Sabouraud agar, the VITEK\u00ae 2 Compact system, and real-time PCR (RT-PCR). (3) Results: C. albicans was the most abundant species (67.5%), followed by Pichia kudriavzevii, Nakaseomyces glabratus, C. parapsilosis, and C. dubliniensis. Fluconazole resistance was observed in two C. albicans isolates, emphasizing the clinical importance of routine antifungal susceptibility testing, and all C. albicans isolates were resistant to micafungin, while amphotericin B remained effective against all isolates. RT-PCR confirmed the presence of C. albicans DNA. (4) Conclusions: The detection of resistant Candida strains highlights the importance of conducting assessments at the species level, which could help clinicians to ensure better antifungal stewardship.<\/jats:p>","DOI":"10.3390\/pathogens14100972","type":"journal-article","created":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T12:48:28Z","timestamp":1758804508000},"page":"972","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Candida Susceptibility to Antifungals in Amniotic Fluid: A Preliminary Study"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-5420-9608","authenticated-orcid":false,"given":"Silvia Gabriela","family":"Ionescu","sequence":"first","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Daniela","family":"Dimitriu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"},{"name":"\u201dCuza-Vod\u0103\u201d Clinical Hospital of Obstetrics and Gynecology, 700038 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demetra Gabriela","family":"Socolov","sequence":"additional","affiliation":[{"name":"\u201dCuza-Vod\u0103\u201d Clinical Hospital of Obstetrics and Gynecology, 700038 Ia\u0219i, Romania"},{"name":"Department of Gynecology and Obstetrics, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela","family":"Grigore","sequence":"additional","affiliation":[{"name":"\u201dCuza-Vod\u0103\u201d Clinical Hospital of Obstetrics and Gynecology, 700038 Ia\u0219i, Romania"},{"name":"Department of Gynecology and Obstetrics, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2592-3982","authenticated-orcid":false,"given":"Luminita Smaranda","family":"Iancu","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1510-3398","authenticated-orcid":false,"given":"Costin","family":"Damian","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxana Gabriela","family":"Cobzaru","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen Valerica","family":"Ripa","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Costin","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radu-Florin","family":"Popa","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brindusa","family":"Copacianu","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Regional Institute of Oncology, 700483 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1811-7923","authenticated-orcid":false,"given":"Ramona Gabriela","family":"Ursu","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine and Interdisciplinarity (IX)\u2014Microbiology, Grigore T. Popa University of Medicine and Pharmacy Ia\u0219i, 700115 Ia\u0219i, Romania"},{"name":"\u201dCuza-Vod\u0103\u201d Clinical Hospital of Obstetrics and Gynecology, 700038 Ia\u0219i, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,25]]},"reference":[{"key":"ref_1","unstructured":"(2022). 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