{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:28:29Z","timestamp":1792092509204,"version":"4.4.0"},"reference-count":22,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:00:00Z","timestamp":1758931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Major Science and Technology Special Project in Anhui Province","award":["202003a06020012"],"award-info":[{"award-number":["202003a06020012"]}]},{"name":"the Anhui Province Poultry Industry Technology System","award":["AHCYJSTX-06"],"award-info":[{"award-number":["AHCYJSTX-06"]}]},{"name":"Team Project of Anhui Academy of Agricultural Sciences","award":["2025YL044"],"award-info":[{"award-number":["2025YL044"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Pathogens"],"abstract":"<jats:p>Duck viral enteritis (DVE) is an acute and highly contagious disease that affects waterfowl such as ducks, geese and swans. Duck enteritis virus (DEV) is the pathogen, causing huge economic losses to waterfowl farming in recent years. Establishing a rapid, simple, and visual detection should facilitate the early identification of DEV. After the amplification primers and reaction conditions were optimized, three multienzyme isothermal rapid amplification (MIRA) methods\u2014basic MIRA, MIRA\u2013quantitative PCR (MIRA\u2013qPCR) and MIRA\u2013lateral flow dipstick (MIRA\u2013LFD)\u2014were established to detect DEV. Specificity analyses showed that the three MIRA methods specifically detected DEV, with no cross-reaction with fowl adenovirus serotype 4, novel goose astrovirus, Muscovy duck reovirus, avian influenza virus subtype H9, or duck circovirus. The basic MIRA reaction was completed in 30 min at 35 \u00b0C, requiring only a pair of primers. Detection with MIRA\u2013qPCR or MIRA\u2013LFD was completed within 20 min, and the limits of detection were 1 \u00d7 101 copies\/\u03bcL for both. MIRA\u2013LFD required no specialized instruments, and the results could be viewed directly with the naked eye. Compared with the traditional PCR, MIRA assays are simple, rapid, and effective and therefore more suitable for the field detection of DEV.<\/jats:p>","DOI":"10.3390\/pathogens14100980","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T16:43:54Z","timestamp":1759250634000},"page":"980","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Rapid Detection of Duck Enteritis Virus with MIRA, MIRA\u2013qPCR, and MIRA\u2013LFD Assays"],"prefix":"10.3390","volume":"14","author":[{"given":"Yin","family":"Dai","sequence":"first","affiliation":[{"name":"Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomiao","family":"Hu","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueyi","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9332-6514","authenticated-orcid":false,"given":"Jieru","family":"Wang","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongdong","family":"Yin","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaocheng","family":"Pan","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelan","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihong","family":"Zhao","sequence":"additional","affiliation":[{"name":"Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety, Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Science, Hefei 230031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1111\/tbed.13002","article-title":"Molecular and pathological characterization of duck enteritis virus in Egypt","volume":"66","author":"Hamed","year":"2019","journal-title":"Transbound. 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