{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:27:33Z","timestamp":1788964053506,"version":"build-2803163510"},"reference-count":62,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:00:00Z","timestamp":1758931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Pathogens"],"abstract":"<jats:p>Nematode parasites of the family Anisakidae can cause human disease when ingested, at the third larval stage (L3), through infected fish or fish products. While human infection is often asymptomatic, in some cases it may present with symptoms ranging from mild to severe gastrointestinal disorders, skin rash, itching, or even anaphylaxis. Ingestion of destroyed L3 (e.g., following processing, sanitisation, or cooking) may still trigger allergic reactions. In the present study, the occurrence of Anisakidae L3 in ready-to-eat (RTE) fish products was investigated. A total of 108 RTE fish products, representing eight species and five processing methods, were examined using (a) macroscopic inspection, (b) artificial digestion (AD), and (c) molecular analysis of both the AD material (to detect non-visible parasite fragments) and the larvae collected by the first two methods. Nematodes morphologically identified as Anisakidae L3 were detected in 32 of the 108 samples (29.62%). Molecular analysis of the AD product did not reveal additional positive samples and confirmed the identification of the isolated larvae as Anisakis simplex sensu stricto and Anisakis pegreffii. Given the public health importance of anisakidosis, the findings of this study provide valuable insight into the prevalence of contamination in RTE fish products currently available on the Greek market.<\/jats:p>","DOI":"10.3390\/pathogens14100981","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T16:43:54Z","timestamp":1759250634000},"page":"981","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Food Safety Concerns: Anisakis spp. in Ready-to-Eat Fish from the Greek Market"],"prefix":"10.3390","volume":"14","author":[{"given":"Evangelia N.","family":"Papapostolou","sequence":"first","affiliation":[{"name":"Department of Food Testing and Research Laboratories of Thessaloniki, Hellenic Food Authority (EFET), 57001 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1539-8237","authenticated-orcid":false,"given":"Serafeim C.","family":"Chaintoutis","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54627 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4471-8594","authenticated-orcid":false,"given":"Panagiota","family":"Gousia","sequence":"additional","affiliation":[{"name":"Department of Food Testing and Research Laboratories of Thessaloniki, Hellenic Food Authority (EFET), 57001 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aggeliki","family":"Karpouza","sequence":"additional","affiliation":[{"name":"Department of Food Testing and Research Laboratories of Thessaloniki, Hellenic Food Authority (EFET), 57001 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9524-0047","authenticated-orcid":false,"given":"Melania","family":"Kachrimanidou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8347-0769","authenticated-orcid":false,"given":"Anastasia","family":"Diakou","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"\u00c1ngeles-Hern\u00e1ndez, J.C., G\u00f3mez-de Anda, F.R., Reyes-Rodr\u00edguez, N.E., Vega-S\u00e1nchez, V., Garc\u00eda-Reyna, P.B., Campos-Montiel, R.G., Calder\u00f3n-Apodaca, N.L., Salgado-Miranda, C., and Zepeda-Vel\u00e1zquez, A.P. 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