{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T05:07:13Z","timestamp":1785215233707,"version":"3.55.0"},"reference-count":60,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T00:00:00Z","timestamp":1759017600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deanship of Scientific Research, Vice Presidency for Graduate Studies and Scientific Research, King Faisal University, Saudi Arabia","award":["KFU 253318"],"award-info":[{"award-number":["KFU 253318"]}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["Pathogens"],"abstract":"<jats:p>Pseudomonas aeruginosa is an opportunistic pathogen of public health concern. This study aimed to investigate the prevalence of P. aeruginosa, some virulence factors, and antimicrobial resistance patterns and highlight the potential pathways of horizontal blaSHV-resistant gene transfer from diverse sources. A total of 220 samples were collected from fish (n = 90), water (n = 30), poultry (n = 50), and humans (n = 50). All samples were isolated, confirmed by the Vitek 2 system, and tested against antimicrobial agents. Some virulence and resistance genes were examined by PCR and sequenced for the blaSHV-resistant gene from four selected isolates from each source. SPSS v26, with chi-squared tests and Pearson correlations (p &lt; 0.05), was implemented for statistical investigation. P. aeruginosa was isolated at 33.3%, 20%, 14%, and 24% from fish, water, poultry, and humans, respectively. Using the diffusion disk method, extensively drug-resistant (XDR) and multidrug-resistant (MDR) strains were detected. All strains harbored the oprL and toxA genes, while the lasB gene was present in 40% of fish samples but not present in human samples. All strains lacked the exoS gene. The tetA, sul1, blaSHV, and blaTEM resistance genes were detected at different percentages. The blaSHV genes from fish and water isolates were closely related to each other and showed similarity to those of the human isolates. The poultry isolates formed a separate phylogenetic lineage. The emergence of XDR and MDR P. aeruginosa highlights a possible public health threat. Based on the gene similarity between fish and water isolates, our results suggest that these isolates have a common origin. The similarity between the human isolates and environmental isolates (fish and water) raises concerns about possible transmission to humans.<\/jats:p>","DOI":"10.3390\/pathogens14100983","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T16:43:54Z","timestamp":1759250634000},"page":"983","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["One Health Monitoring of Resistant Pseudomonas aeruginosa in Aquatic, Poultry, and Human Sources: Virulence Traits and blaSHV Gene Tracking"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5524-8335","authenticated-orcid":false,"given":"Ali","family":"Wahdan","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmoud","family":"Ezzat","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Emam","sequence":"additional","affiliation":[{"name":"Veterinary Medicine Directorate, Ministry of Agriculture, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1589-3377","authenticated-orcid":false,"given":"Walaa A.","family":"Husseiny","sequence":"additional","affiliation":[{"name":"Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Abou El-Atta","sequence":"additional","affiliation":[{"name":"Central Lab for Aquaculture Research, Abbassa 44662, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehab M.","family":"Abd-Allah","sequence":"additional","affiliation":[{"name":"Veterinary Hospital, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6569-1365","authenticated-orcid":false,"given":"Ahmed M. A.","family":"Meligy","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Central Lab, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7312-3413","authenticated-orcid":false,"given":"Sherief M.","family":"Abdel-Raheem","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faisal","family":"Almathen","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6928-7447","authenticated-orcid":false,"given":"Salah","family":"Al-Shami","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saad I.","family":"Al-Sultan","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7956-1561","authenticated-orcid":false,"given":"Ahmed","family":"Alfifi","sequence":"additional","affiliation":[{"name":"Department of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2451-296X","authenticated-orcid":false,"given":"Wael","family":"El-Deeb","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, Central Lab, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf 31982, Al-Ahsa, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0759-3094","authenticated-orcid":false,"given":"Marwa E.","family":"Abo Hashem","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Immunology, and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Abou Elez, R.M.M., Zahra, E.M.F., Gharieb, R.M.A., Mohamed, M.E.M., Samir, M., Saad, A.M., and Merwad, A.M.A. (2024). Resistance patterns, virulence determinants, and biofilm genes of multidrug-resistant P. aeruginosa isolated from fish and fish handlers. Sci. 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