{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T00:47:22Z","timestamp":1761094042265,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T00:00:00Z","timestamp":1760832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundaci\u00f3n Alfonso X el Sabio","award":["1.01.911"],"award-info":[{"award-number":["1.01.911"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Calicophoron daubneyi, a rumen fluke increasingly reported in European livestock, has emerged as a relevant parasitic threat in cattle. This study investigated the prevalence and seasonal dynamics of gastrointestinal endoparasites in 382 fecal samples from 40 beef cattle farms (26 extensive and 14 semi-extensive) in central Spain. Samples were analyzed using flotation, sedimentation, and modified McMaster techniques, complemented by PCR confirmation of trematodes and a 25-variable epidemiological survey. C. daubneyi was detected in 38.74% of samples and 77.5% of farms, surpassing Fasciola hepatica (13.09%), gastrointestinal nematodes (42.15%), and Eimeria spp. (16.75%). Mixed infections were frequent. Seasonal shedding patterns varied by parasite, with C. daubneyi peaking in spring and winter. Statistical analyses (Kruskal\u2013Wallis, ANOVA, Mann\u2013Whitney U) revealed significant seasonal differences and confirmed higher F. hepatica egg counts in extensive systems (p = 0.0012). Anthelmintic treatment was infrequent and mainly guided by coprological diagnosis; ivermectin, closantel, albendazole, and nitroxinil were the most used drugs, though none fully effective against C. daubneyi. Anthelmintic resistance was not evaluated in this study. These findings confirm the emergence of C. daubneyi in central Spain and highlight the need for targeted surveillance and seasonally adjusted control strategies.<\/jats:p>","DOI":"10.3390\/pathogens14101057","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T08:18:54Z","timestamp":1760948334000},"page":"1057","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Gastrointestinal Endoparasites in Cattle in Central Spain: Focus on Calicophoron daubneyi with Coprological, Epidemiological, and Anthelmintic Insights"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2079-5942","authenticated-orcid":false,"given":"N\u00e9lida Fern\u00e1ndez","family":"Pato","sequence":"first","affiliation":[{"name":"Facultad de Veterinaria, Universidad Alfonso X el Sabio (UAX), Villanueva de la Ca\u00f1ada, 28691 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d3scar Garc\u00eda","family":"Barrero","sequence":"additional","affiliation":[{"name":"Independent Clinical Veterinarian, 40001 Segovia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elvira Rodr\u00edguez","family":"Velasco","sequence":"additional","affiliation":[{"name":"Independent Clinical Veterinarian, 40001 Segovia, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5609-0496","authenticated-orcid":false,"given":"F\u00e9lix Valc\u00e1rcel","family":"Sancho","sequence":"additional","affiliation":[{"name":"Parasitology Group, Animal Reproduction Department INIA-CSIC, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5846-3581","authenticated-orcid":false,"given":"Jaime Gal\u00e1n","family":"Elvira","sequence":"additional","affiliation":[{"name":"Facultad de Veterinaria, Universidad Alfonso X el Sabio (UAX), Villanueva de la Ca\u00f1ada, 28691 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Terfa, W., Kumsa, B., Ayana, D., Maurizio, A., Tessarin, C., and Cassini, R. 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