{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:01:38Z","timestamp":1787029298498,"version":"build-2736575974"},"reference-count":31,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T00:00:00Z","timestamp":1762819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Human adenoviruses (HAdVs; genus Mastadenovirus, family Adenoviridae) are major etiologic agents of respiratory infections, exerting a disproportionately large impact on children. However, no long-term study to date has spanned pre- and post-pandemic periods in a Korean tertiary setting. Here, we retrospectively analyzed 23,284 nasopharyngeal swabs collected between 2007 and 2024 at a tertiary medical center in South Korea. Most specimens were obtained from inpatients and outpatients presenting with fever or respiratory symptoms for differential viral diagnosis. HAdV was detected using real-time PCR, and positivity rates were compared by year, season, and age group. Overall, 2043 tested positive for HAdV. Annual positivity peaked in 2010, 2013, and 2016, followed by a sharp decline during the coronavirus disease 2019 pandemic, reaching its lowest level in 2024. Positivity was higher in summer and spring than in winter. Children aged 1\u20135 years had the highest positivity, whereas adults and older adults showed markedly lower rates. The disproportionate burden among children underscores the need for targeted surveillance, school-based infection control, and region-specific preparedness strategies. These findings provide crucial long-term evidence into adenovirus circulation in the post-pandemic era and provides an evidence-based foundation for future public health policy and infection control planning.<\/jats:p>","DOI":"10.3390\/pathogens14111143","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T08:57:41Z","timestamp":1762851461000},"page":"1143","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Long-Term Epidemiological Trends of Human Adenovirus Infection in South Korea: A Single-Center Study (2007\u20132024)"],"prefix":"10.3390","volume":"14","author":[{"given":"Yu Jeong","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Dankook University, Cheonan-si 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0934-8445","authenticated-orcid":false,"given":"Sung Hun","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Medical Laser, Graduate School of Medicine, Dankook University, Cheonan-si 31116, Republic of Korea"},{"name":"Research Center for Bio-Functional and Biocompatible Materials, Dankook University, Cheonan-si 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7003-8422","authenticated-orcid":false,"given":"Jeong Su","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Dankook University, Cheonan-si 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8981-5705","authenticated-orcid":false,"given":"Jae-Sik","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Dankook University, Cheonan-si 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1534-563X","authenticated-orcid":false,"given":"Jae Kyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical Laboratory Science, College of Health Sciences, Dankook University, Cheonan-si 31116, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e71010","DOI":"10.1002\/hsr2.71010","article-title":"Addressing the health risks posed by adenovirus: A perspective on strategies for prevention and management","volume":"8","author":"Urmi","year":"2025","journal-title":"Health Sci. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"671","DOI":"10.21037\/tp-2024-627","article-title":"Association of human adenovirus load and viral genotype diversity with respiratory disease severity in children: A systematic review and meta-analysis","volume":"14","author":"Zhang","year":"2025","journal-title":"Transl. Pediatr."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Soler Wenglein, J.S., Scarsella, L., Kotlewski, C., Heim, A., and Aydin, M. (2025). Current trends of human adenovirus types among hospitalized children\u2014A systematic review. Viruses, 17.","DOI":"10.3390\/v17070914"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"106475","DOI":"10.1016\/j.jinf.2025.106475","article-title":"Increased circulation of adenovirus in China during 2023\u20132024: Association with an increased prevalence of species B and school-associated transmission","volume":"90","author":"Jin","year":"2025","journal-title":"J. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.virs.2022.01.010","article-title":"Analysis of severe human adenovirus infection outbreak in Guangdong Province, southern China in 2019","volume":"37","author":"Liu","year":"2022","journal-title":"Virol. Sin."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"581","DOI":"10.3343\/alm.2023.0484","article-title":"Increased trend of adenovirus activity after the COVID-19 pandemic in South Korea: Analysis of national surveillance data","volume":"44","author":"Lee","year":"2024","journal-title":"Ann. Lab. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e29780","DOI":"10.1002\/jmv.29780","article-title":"A significant outbreak of respiratory human adenovirus infections among children aged 3\u20136 years in Hokkaido, Japan, in 2023","volume":"96","author":"Fukuda","year":"2024","journal-title":"J. Med. Virol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1007\/s12519-022-00590-w","article-title":"Human adenovirus (HAdV) infection in children with acute respiratory tract infections in Guangzhou, China, 2010\u20132021: A molecular epidemiology study","volume":"18","author":"Chen","year":"2022","journal-title":"World J. Pediatr."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Abdullah, O., Fall, A., Klein, E., and Mostafa, H.H. (2024). Increased circulation of human adenovirus in 2023: An investigation of the circulating genotypes, upper respiratory viral loads, and hospital admissions in a large academic medical center. J. Clin. Microbiol., 62.","DOI":"10.1128\/jcm.01237-23"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1128\/CMR.00116-13","article-title":"Adenovirus infections in immunocompetent and immunocompromised patients","volume":"27","author":"Lion","year":"2014","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Aganovic, A., Bi, Y., Cao, G., Kurnitski, J., and Wargocki, P. (2022). Modeling the impact of indoor relative humidity on the infection risk of five respiratory airborne viruses. Sci. Rep., 12.","DOI":"10.1038\/s41598-022-15703-8"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"199558","DOI":"10.1016\/j.virusres.2025.199558","article-title":"The epidemiological analysis of respiratory virus infections in children in Hangzhou from 2019 to 2023","volume":"355","author":"Zhao","year":"2025","journal-title":"Virus Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"100190","DOI":"10.1016\/j.jcvp.2024.100190","article-title":"Epidemiology of respiratory viruses before and during the COVID-19 pandemic in a tertiary care hospital in Southern Brazil","volume":"4","author":"Richter","year":"2024","journal-title":"J. Clin. Virol. Plus"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"119234","DOI":"10.1016\/j.ecoenv.2025.119234","article-title":"High humidity exposures and mechanisms in respiratory disease","volume":"305","author":"Xuan","year":"2025","journal-title":"Ecotoxicol. Environ. Saf."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s12985-023-01962-y","article-title":"The changed endemic pattern of human adenovirus from species B to C among pediatric patients under the pressure of non-pharmaceutical interventions against COVID-19 in Beijing, China","volume":"20","author":"Wang","year":"2023","journal-title":"Virol. J."},{"key":"ref_16","first-page":"100969","article-title":"Comparison of control and transmission of COVID-19 across epidemic waves in Hong Kong: An observational study","volume":"43","author":"Yang","year":"2024","journal-title":"Lancet Reg. Health West. Pac."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2001600","DOI":"10.2807\/1560-7917.ES.2021.26.29.2001600","article-title":"Decline of influenza and respiratory syncytial virus detection in facility-based surveillance during the COVID-19 pandemic, South Africa, January to October 2020","volume":"26","author":"Tempia","year":"2021","journal-title":"Eurosurveillance"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Liang, D.F., Guo, W.L., Zhu, D.P., Li, S.Y., Zhu, W.D., Li, Y., Huang, L., Shen, J., and Li, P.Q. (2025). Changes in the epidemic patterns of respiratory pathogens of children in Guangzhou, China during the COVID-19 pandemic. BMC Infect. Dis., 25.","DOI":"10.1186\/s12879-025-11215-8"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e171","DOI":"10.3346\/jkms.2024.39.e171","article-title":"Epidemiology of respiratory viruses in Korean children before and after the COVID-19 pandemic: A prospective study from national surveillance system","volume":"39","author":"Cho","year":"2024","journal-title":"J. Korean Med. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Choi, Y.J., Chung, E.H., Lee, E., Kim, C.H., Lee, Y.J., Kim, H.B., Kim, B.S., Kim, H.Y., Cho, Y., and Seo, J.H. (2022). Clinical characteristics of macrolide-refractory Mycoplasma pneumoniae pneumonia in Korean children: A multicenter retrospective study. J. Clin. Med., 11.","DOI":"10.3390\/jcm11020306"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1111\/irv.12998","article-title":"International changes in respiratory syncytial virus (RSV) epidemiology during the COVID-19 pandemic: Association with school closures","volume":"16","author":"Billard","year":"2022","journal-title":"Influ. Other Respir. Viruses"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e71","DOI":"10.3346\/jkms.2023.38.e71","article-title":"The changes in respiratory and enteric adenovirus epidemiology in Korea from 2017 to June 2022","volume":"38","author":"Kim","year":"2023","journal-title":"J. Korean Med. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Thindwa, D., Li, K., Cooper-Wootton, D., Zheng, Z., Pitzer, V.E., and Weinberger, D.M. (2024). Global patterns of rebound to normal RSV dynamics following COVID-19 suppression. BMC Infect. Dis., 24.","DOI":"10.1186\/s12879-024-09509-4"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1016\/j.jmii.2024.06.004","article-title":"The real-world impact of the BioFire FilmArray blood culture identification 2 panel on antimicrobial stewardship among patients with bloodstream infections in intensive care units with a high burden of drug-resistant pathogens","volume":"57","author":"Chen","year":"2024","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1002\/ped4.12396","article-title":"Clinical epidemiology and disease burden of adenoviral encephalitis in hospitalized children in China: A nationwide cross-sectional study","volume":"7","author":"Tian","year":"2023","journal-title":"Pediatr. Investig."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e2203019119","DOI":"10.1073\/pnas.2203019119","article-title":"Simulating respiratory disease transmission within and between classrooms to assess pandemic management strategies at schools","volume":"119","author":"Endo","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Lv, Q., Luo, R., Li, X., Deng, S., Liu, K., Liu, L., Zhu, K., Wen, Y., Ma, X., and Ci, R. (2025). Risk assessment of communicable respiratory diseases transmission based on social contact networks: A primary school contact data survey conducted with portable high-precision devices. BMC Public Health, 25.","DOI":"10.1186\/s12889-025-24327-2"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"ofad500-935","DOI":"10.1093\/ofid\/ofad500.935","article-title":"890. Environmental air and surface sampling of respiratory viruses in child care centers","volume":"10","author":"Chedid","year":"2023","journal-title":"Open Forum Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"He, Y., Liu, W.J., Jia, N., Richardson, S., and Huang, C. (2023). Viral respiratory infections in a rapidly changing climate: The need to prepare for the next pandemic. eBioMedicine, 93.","DOI":"10.1016\/j.ebiom.2023.104593"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Tanimoto, Y., Ohyama, M., Ito, E., Akiyoshi, K., Onishi, Y., Mori, A., and Nomoto, R. (2025). Whole genome-based surveillance for human adenovirus-related diseases in Kobe City, Japan, 2018\u20132022. BMC Res. Notes, 18.","DOI":"10.1186\/s13104-025-07225-z"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, C., Xiao, Y., Zhang, J., Ren, L., Li, J., Xie, Z., Xu, B., Yang, Y., Qian, S., and Wang, J. (2015). Adenovirus infection in children with acute lower respiratory tract infections in Beijing, China, 2007 to 2012. BMC Infect. Dis., 15.","DOI":"10.1186\/s12879-015-1126-2"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/11\/1143\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T09:18:30Z","timestamp":1762852710000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/11\/1143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,11]]},"references-count":31,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,11]]}},"alternative-id":["pathogens14111143"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14111143","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,11]]}}}