{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T05:52:23Z","timestamp":1763617943242,"version":"3.45.0"},"reference-count":95,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2022\/08543-2"],"award-info":[{"award-number":["2022\/08543-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"crossref","award":["303701\/2021-8"],"award-info":[{"award-number":["303701\/2021-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordination for the Improvement of Higher Education Personnel","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Blood transfusions are indispensable in Veterinary Medicine, providing therapeutic support in cases of hematological disorders. Several pathogens can cause disease and\/or exacerbate the condition of immunocompromised dogs or those requiring a transfusion. This study aimed to investigate the molecular occurrence of hemopathogens (Bartonella spp., Ehrlichia spp., Anaplasma spp., piroplasmids, and hemoplasmas) in blood donor and patient dogs using samples from a clinical veterinary laboratory in Brazil. One hundred blood samples were collected from each group. All dogs tested negative for Bartonella spp. in all performed assays. Among the 100 dogs from the clinical veterinary laboratory, 15% (95% CI: 9.3\u201323.3) tested positive for Ehrlichia spp., 6% (95% CI: 2.8\u201312.5) for Anaplasma spp., 3% (95% CI: 1.0\u20138.5) for Babesia spp., and 2% (95% CI: 0.6\u20137.0) for hemoplasmas. Blood donor dogs tested positive for hemoplasmas (5%) (95% CI: 2.2\u201311.2). Additional conventional and real-time PCR assays followed by sequencing confirmed the presence of Ehrlichia canis, Anaplasma platys, Babesia vogeli, \u2018Candidatus Mycoplasma haematoparvum\u2019, and Mycoplasma haemocanis. The molecular detection of E. canis, A. platys, \u2018Ca. M. haematoparvum\u2019, and M. haemocanis in dogs from midwestern Brazil reinforces the relevance of molecular tools in diagnosing hemopathogens. This is the first molecular detection of hemoplasmas in canine blood donors from Brazil. This finding indicates their silent circulation and highlights the importance of molecular screening to prevent the worsening of clinical conditions and the risk of turning recipients into new sources of infection.<\/jats:p>","DOI":"10.3390\/pathogens14111180","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T12:33:04Z","timestamp":1763469184000},"page":"1180","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Survey of Hemopathogens in Dogs, Including Blood Donors, from Central-Western Brazil"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6462-4873","authenticated-orcid":false,"given":"Jo\u00e3o Vitor dos Santos Alves","family":"da Silva","sequence":"first","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences (FCAV), S\u00e3o Paulo State University (UNESP), Jaboticabal Campus, Jaboticabal 14884-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorena Freitas","family":"das Neves","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences (FCAV), S\u00e3o Paulo State University (UNESP), Jaboticabal Campus, Jaboticabal 14884-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eduarda","family":"Bolzan","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences (FCAV), S\u00e3o Paulo State University (UNESP), Jaboticabal Campus, Jaboticabal 14884-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9246-3529","authenticated-orcid":false,"given":"Liliane Maria do Rosario","family":"Batista","sequence":"additional","affiliation":[{"name":"OHV One Health Veterinary\u2014Centro de Hemoterapia Pet, Bras\u00edlia 71736-501, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3185-8331","authenticated-orcid":false,"given":"Francisco Anilton Alves","family":"Araujo","sequence":"additional","affiliation":[{"name":"OHV One Health Veterinary\u2014Centro de Hemoterapia Pet, Bras\u00edlia 71736-501, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences (FCAV), S\u00e3o Paulo State University (UNESP), Jaboticabal Campus, Jaboticabal 14884-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-5222","authenticated-orcid":false,"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences (FCAV), S\u00e3o Paulo State University (UNESP), Jaboticabal Campus, Jaboticabal 14884-900, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1053\/j.ctsap.2004.01.007","article-title":"Collection and preparation of blood products","volume":"19","author":"Lucas","year":"2004","journal-title":"Clin. 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