{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:04:08Z","timestamp":1790805848252,"version":"4.1.0"},"reference-count":30,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,11,23]],"date-time":"2025-11-23T00:00:00Z","timestamp":1763856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"PROGETTI DI RICERCA CORRENTE 2021","award":["IZS ME 09-21 RC"],"award-info":[{"award-number":["IZS ME 09-21 RC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Feline leishmaniosis (FeL) is still considered an emerging and neglected disease. Cats, once considered accidental hosts, are now recognized as adjunctive reservoirs of the disease, especially in areas where canine (CanL) and human (HumL) leishmaniosis are widespread. Although often asymptomatic, infected cats could contribute to the transmission cycle of the parasite. Recent studies in Campania (Italy) have found a significant prevalence of feline infection, indicating the need to implement diagnostic and surveillance protocols to prevent the spread of the disease. The aim of the study was to outline the current scenario by studying the prevalence of FeL in Campania to identifying the potential zoonotic risk and in addition to validate the Immunofluorescence Antibody Test (IFAT) method for the diagnosis of leishmaniosis in cats. The study involved initially 702 cats; for each cat, a clinical record was compiled, including identification data, anamnesis, and clinical findings. Due to incomplete information, statistical analysis was performed only on a subset of 601 cats. A blood sample was collected to obtain serum\/plasma specimens. When feasible, a lymph node fine-needle aspiration was performed. The observed seroprevalence rate was 32.1% (193\/601), with a higher seroprevalence in outdoor cats and the presence of asymptomatic seropositive animals (28.0%;54\/193), suggesting that felines may act as silent reservoirs of Leishmania infantum. An excellent result was obtained for the validation and standardization of the analytical IFAT method for the diagnosis of feline leishmaniasis; therefore, an inter-laboratory test has been carried out to establish the dilution cut-off at \u22651:80 as compatible with infection. Furthermore, a xenodiagnosis examination was conducted on a cat that was infected to more accurately evaluate the possibility of asymptomatic cats acting as carriers of the infection; however, this test resulted negative.<\/jats:p>","DOI":"10.3390\/pathogens14121194","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T09:02:07Z","timestamp":1763974927000},"page":"1194","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High Seroprevalence of Feline Leishmaniosis (FeL) in Campania (Italy) Region: Current Epidemiological Scenario"],"prefix":"10.3390","volume":"14","author":[{"given":"Valentina","family":"Foglia Manzillo","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Balestrino","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetano","family":"Oliva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1597-9322","authenticated-orcid":false,"given":"Roberta","family":"Brunetti","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy (IZSM), Via Salute 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5695-8005","authenticated-orcid":false,"given":"Stefania","family":"Cavallo","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy (IZSM), Via Salute 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6172-3446","authenticated-orcid":false,"given":"Rosa","family":"D\u2019Ambrosio","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy (IZSM), Via Salute 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8416-996X","authenticated-orcid":false,"given":"Roberta","family":"Pellican\u00f2","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy (IZSM), Via Salute 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luisa","family":"Spadari","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy (IZSM), Via Salute 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4513-0018","authenticated-orcid":false,"given":"Lorella","family":"Barca","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy (IZSM), Via Salute 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6181-6984","authenticated-orcid":false,"given":"Federica","family":"Bruno","sequence":"additional","affiliation":[{"name":"National Reference Center for Leishmaniosis (C.Re.Na.L.), OIE Leishmania Reference Laboratory, Experimental Zooprophylactic Institutes of Sicily (IZS Sicilia), Via Gino Marinuzzi 3, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8391-9255","authenticated-orcid":false,"given":"Maria Ortensia","family":"Montella","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7564-3356","authenticated-orcid":false,"given":"Maria Paola","family":"Maurelli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nunzia","family":"Florindo","sequence":"additional","affiliation":[{"name":"Local Health Authority (ASL) Napoli 3 Sud, U.O.S. Clinica Veterinaria, Via Calastro 26, 80059 Torre del Greco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3362-5395","authenticated-orcid":false,"given":"Manuela","family":"Gizzarelli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0466-5563","authenticated-orcid":false,"given":"Mariele","family":"De Santi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Via Federico Delpino 1, 80137 Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3087-7148","authenticated-orcid":false,"given":"Loredana","family":"Baldi","sequence":"additional","affiliation":[{"name":"Experimental Zooprophylactic Institute of Southern Italy (IZSM), Via Salute 2, 80055 Portici, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.vetpar.2014.12.023","article-title":"Leishmaniosis of companion animals in Europe: An update","volume":"208","author":"Pennisi","year":"2015","journal-title":"Vet. 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