{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T13:57:50Z","timestamp":1764943070798,"version":"3.46.0"},"reference-count":68,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"AquaGen AS","doi-asserted-by":"publisher","award":["321428"],"award-info":[{"award-number":["321428"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"The Research Council of Norway","doi-asserted-by":"publisher","award":["333399"],"award-info":[{"award-number":["333399"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The infectious pancreatic necrosis virus (IPNV) used to be one of the largest loss factors in Atlantic salmon farming. Since 2009, marker-assisted selection for resistance to IPN, targeting a single major quantitative trait locus (QTL), has led to a ten-fold decrease in the number of IPN outbreaks in Norway. However, some IPN-related problems remain, due to isolates of the virus which seem to bypass the resistance mechanism of the QTL. We comparatively characterized a classical isolate affected by the IPN-QTL (cIPNV) and an isolate that circumvents the QTL-based protection (rIPNV). Using both in vivo and in vitro challenges, the viral infection dynamics and host responses were evaluated by RT-qPCR and by gene ontology (GO) enrichment analysis from the RNA sequencing data of infected hepatocytes and the whole liver. Overall, cIPNV showed rapid replication with pronounced lytic cytopathology and enrichment for DNA damage, apoptosis and cell cycle disruption GO terms, while rIPNV exhibited slower accumulation of viral RNA and a transcriptional footprint consistent with pro-survival states in hepatocytes. While further research is needed to resolve the causality of QTL evasion, this work provides a first characterization of the pathogenicity of emerging QTL-insensitive IPNV isolates.<\/jats:p>","DOI":"10.3390\/pathogens14121245","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T13:17:07Z","timestamp":1764940627000},"page":"1245","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Infection Dynamics and Host Responses to Two IPNV Isolates in Liver of Atlantic Salmon (Salmo salar)"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8390-8666","authenticated-orcid":false,"given":"Valeria","family":"Aguilar Qui\u00f1ones","sequence":"first","affiliation":[{"name":"AquaGen AS, 7462 Trondheim, Norway"},{"name":"Centre for Integrative Genomics (CIGENE), Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, 1432 \u00c5s, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9551-9280","authenticated-orcid":false,"given":"Fabian","family":"Grammes","sequence":"additional","affiliation":[{"name":"AquaGen AS, 7462 Trondheim, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Boyartchuk","sequence":"additional","affiliation":[{"name":"Centre for Integrative Genomics (CIGENE), Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, 1432 \u00c5s, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacob Seil\u00f8","family":"Torgersen","sequence":"additional","affiliation":[{"name":"AquaGen AS, 7462 Trondheim, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1111\/j.1365-2761.2005.00642.x","article-title":"Infectious Pancreatic Necrosis in Atlantic Salmon, Salmo salar L.","volume":"28","author":"Roberts","year":"2005","journal-title":"J. Fish Dis."},{"key":"ref_2","unstructured":"Wolf, K. (1988). Fish Viruses and Fish Viral Diseases, Cornell University Press."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Roberts, R.J. (2012). Fish Pathology, Wiley-Blackwell. [4th ed.].","DOI":"10.1002\/9781118222942"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Munro, E.S., and Midtlyng, P.J. (2011). Infectious Pancreatic Necrosis and Associated Aquatic Birnaviruses. Fish Diseases and Disorders. Volume 3: Viral, Bacterial and Fungal Infections, CABI.","DOI":"10.1079\/9781845935542.0001"},{"key":"ref_5","unstructured":"Terio, K.A., McAloose, D., and Leger, J.S. (2018). Chapter 39\u2014Osteichthyes. Pathology of Wildlife and Zoo Animals, Academic Press."},{"key":"ref_6","unstructured":"Bruno, D.W., Poppe, T.T., and Noguera Patricia, A. (1996). A Colour Atlas of Salmonid Diseases, Academic Press."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1297-9716-44-7","article-title":"Antigen Dose and Humoral Immune Response Correspond with Protection for Inactivated Infectious Pancreatic Necrosis Virus Vaccines in Atlantic Salmon (Salmo salar L)","volume":"44","author":"Fredriksen","year":"2013","journal-title":"Vet. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/j.vaccine.2012.04.039","article-title":"Comparison of Vaccine Efficacy for Different Antigen Delivery Systems for Infectious Pancreatic Necrosis Virus Vaccines in Atlantic Salmon (Salmo salar L.) in a Cohabitation Challenge Model","volume":"30","author":"Fredriksen","year":"2012","journal-title":"Vaccine"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1365-2761.2007.00866.x","article-title":"Isolation and Quantification of Infectious Pancreatic Necrosis Virus from Ovarian and Seminal Fluids of Atlantic Salmon, Salmo salar L.","volume":"31","author":"Smail","year":"2008","journal-title":"J. Fish Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1099\/jgv.0.001185","article-title":"ICTV Virus Taxonomy Profile: Birnaviridae","volume":"100","author":"Delmas","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1128\/jvi.32.2.593-605.1979","article-title":"Biophysical and Biochemical Characterization of Five Animal Viruses with Bisegmented Double-Stranded RNA Genomes","volume":"32","author":"Dobos","year":"1979","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2231","DOI":"10.1099\/vir.0.80012-0","article-title":"Peptides Resulting from the pVP2 C-Terminal Processing Are Present in Infectious Pancreatic Necrosis Virus Particles","volume":"85","author":"Galloux","year":"2004","journal-title":"J. Gen. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"6652","DOI":"10.1128\/JVI.02831-06","article-title":"VP3, a Structural Protein of Infectious Pancreatic Necrosis Virus, Interacts with RNA-Dependent RNA Polymerase VP1 and with Double-Stranded RNA","volume":"81","author":"Pedersen","year":"2007","journal-title":"J. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1006\/viro.2001.1336","article-title":"IPNV VP5, a Novel Anti-Apoptosis Gene of the Bcl-2 Family, Regulates Mcl-1 and Viral Protein Expression","volume":"295","author":"Hong","year":"2002","journal-title":"Virology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.virol.2005.07.028","article-title":"Infectious Pancreatic Necrosis Virus Induces Apoptosis in Vitro and in Vivo Independent of VP5 Expression","volume":"342","author":"Santi","year":"2005","journal-title":"Virology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.virusres.2009.03.004","article-title":"The Interplay between Infectious Pancreatic Necrosis Virus (IPNV) and the IFN System: IFN Signaling Is Inhibited by IPNV Infection","volume":"143","author":"Skjesol","year":"2009","journal-title":"Virus Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/0959-8030(95)00003-8","article-title":"The Molecular Biology of Infectious Pancreatic Necrosis Virus (IPNV)","volume":"5","author":"Dobos","year":"1995","journal-title":"Annu. Rev. Fish Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.virusres.2017.07.014","article-title":"Study of RNA-A Initiation Translation of The Infectious Pancreatic Necrosis Virus","volume":"240","author":"Jorquera","year":"2017","journal-title":"Virus Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6684304","DOI":"10.1155\/2023\/6684304","article-title":"A Single Mutation of VP2 Is Responsible for the Lethality and Antigenicity Differences between Novel Variant and Very Virulent IBDV Strains","volume":"2023","author":"Jiang","year":"2023","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_20","first-page":"85","article-title":"Infectious Bursal Disease Virus in Chickens: Prevalence, Impact, and Management Strategies","volume":"10","author":"Dey","year":"2019","journal-title":"Vet. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1637\/10448-110812-Reg.1","article-title":"Persistence and Tissue Distribution of Infectious Bursal Disease Virus in Experimentally Infected SPF and Commercial Broiler Chickens","volume":"57","author":"Abdul","year":"2013","journal-title":"Avian Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1111\/jfd.12317","article-title":"Persistent Infections with Infectious Pancreatic Necrosis Virus (IPNV) of Different Virulence in Atlantic Salmon, Salmo salar L.","volume":"38","author":"Julin","year":"2015","journal-title":"J. Fish Dis."},{"key":"ref_23","unstructured":"OIE Aquatic Animal Health Standards Commission (2005). Report of the Meeting of the OIE Aquatic Animal Health Standards Commission, World Organization for Animal Health."},{"key":"ref_24","unstructured":"Sommerset, I., Wiik-Nielsen, J., Moldal, T., Oliveira, V.H.S., Svendsen, J.C., Haukaas, A., and Brun, E. (2024). Norwegian Fish Health Report 2023, Norwegian Veterinary Institute."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1109","DOI":"10.1534\/genetics.107.082974","article-title":"Major Quantitative Trait Loci Affect Resistance to Infectious Pancreatic Necrosis in Atlantic Salmon (Salmo salar)","volume":"178","author":"Houston","year":"2008","journal-title":"Genetics"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Moen, T., Baranski, M., Sonesson, A.K., and Kj\u00f8glum, S. (2009). Confirmation and Fine-Mapping of a Major QTL for Resistance to Infectious Pancreatic Necrosis in Atlantic Salmon (Salmo salar): Population-Level Associations between Markers and Trait. BMC Genom., 10.","DOI":"10.1186\/1471-2164-10-368"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hillestad, B., Johannessen, S., Melingen, G.O., and Moghadam, H.K. (2021). Identification of a New Infectious Pancreatic Necrosis Virus (IPNV) Variant in Atlantic Salmon (Salmo salar L.) That Can Cause High Mortality Even in Genetically Resistant Fish. Front. Genet., 12.","DOI":"10.3389\/fgene.2021.635185"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Benkaroun, J., Muir, K.F., Allshire, R., Tamer, C., and Weidmann, M. (2021). Isolation of a New Infectious Pancreatic Necrosis Virus (IPNV) Variant from a Fish Farm in Scotland. Viruses, 13.","DOI":"10.3390\/v13030385"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Godoy, M., Kibenge, M.J.T., Montes de Oca, M., Pontigo, J.P., Coca, Y., Caro, D., Kusch, K., Suarez, R., Burbulis, I., and Kibenge, F.S.B. (2022). Isolation of a New Infectious Pancreatic Necrosis Virus (IPNV) Variant from Genetically Resistant Farmed Atlantic Salmon (Salmo salar) during 2021\u20132022. Pathogens, 11.","DOI":"10.3390\/pathogens11111368"},{"key":"ref_30","unstructured":"Moldal, T., Wiik-Nielsen, J., Oliveira, V.H.S., Svendsen, J.C., and Sommerset, I. (2025). Norwegian Fish Health Report 2024, Norwegian Veterinary Institute."},{"key":"ref_31","unstructured":"Hjeltnes, B. (2014). Fish Health Report 2013, Norwegian Veterinary Institute."},{"key":"ref_32","unstructured":"Johansen, R., Kongtorp, R.T., Born\u00f8, G., Ringkj\u00f8b Skjelstad, H., Olsen, A.B., Flesj\u00e5, K., Colquhoun, D., \u00d8rpetveit, I., Hansen, H., and Garseth, \u00c5.H. (2009). The Health Situation in Farmed Salmonids 2008, Norwegian Veterinary Institute."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Gjessing, M.C., Aamelfot, M., Batts, W.N., Benestad, S.L., Dale, O.B., Thoen, E., Weli, S.C., and Winton, J.R. (2018). Development and Characterization of Two Cell Lines from Gills of Atlantic Salmon. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0191792"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A Simple Method of Estimating Fifty Per Cent Endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"89","DOI":"10.3354\/dao045089","article-title":"Phylogenetic Relationships of Aquatic Birnaviruses Based on Deduced Amino Acid Sequences of Genome Segment A cDNA","volume":"45","author":"Blake","year":"2001","journal-title":"Dis. Aquat. Org."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: Recent Updates to the Phylogenetic Tree Display and Annotation Tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"jkad039","DOI":"10.1093\/g3journal\/jkad039","article-title":"Efficient Transfection of Atlantic Salmon Primary Hepatocyte Cells for Functional Assays and Gene Editing","volume":"13","author":"Datsomor","year":"2023","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"33","DOI":"10.12688\/f1000research.29032.2","article-title":"Sustainable Data Analysis with Snakemake","volume":"10","author":"Jablonski","year":"2021","journal-title":"F1000Research"},{"key":"ref_39","unstructured":"Simon, A. (FastQC, 2010). FastQC, version 0.10; Quality Control Tool for High Throughput Sequence Data."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt Removes Adapter Sequences from High-Throughput Sequencing Reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet. J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1093\/bioinformatics\/bts635","article-title":"STAR: Ultrafast Universal RNA-Seq Aligner","volume":"29","author":"Dobin","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Law, C.W., Chen, Y., Shi, W., and Smyth, G.K. (2014). Voom: Precision Weights Unlock Linear Model Analysis Tools for RNA-Seq Read Counts. Genome Biol., 15.","DOI":"10.1186\/gb-2014-15-2-r29"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"W207","DOI":"10.1093\/nar\/gkad347","article-title":"G:Profiler\u2014Interoperable Web Service for Functional Enrichment Analysis and Gene Identifier Mapping (2023 Update)","volume":"51","author":"Kolberg","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1101\/gr.1239303","article-title":"Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks","volume":"13","author":"Shannon","year":"2003","journal-title":"Genome Res."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Merico, D., Isserlin, R., Stueker, O., Emili, A., and Bader, G.D. (2010). Enrichment Map: A Network-Based Method for Gene-Set Enrichment Visualization and Interpretation. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0013984"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/s002399910007","article-title":"Rates of Conservative and Radical Nonsynonymous Nucleotide Substitutions in Mammalian Nuclear Genes","volume":"50","author":"Zhang","year":"2000","journal-title":"J. Mol. Evol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.virol.2003.12.016","article-title":"Identification of Putative Motifs Involved in the Virulence of Infectious Pancreatic Necrosis Virus","volume":"322","author":"Santi","year":"2004","journal-title":"Virology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3354\/dao061023","article-title":"Molecular Characterization of Sp Serotype Strains of Infectious Pancreatic Necrosis Virus Exhibiting Differences in Virulence","volume":"61","author":"Shivappa","year":"2004","journal-title":"Dis. Aquat. Org."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.vetimm.2010.02.015","article-title":"The Persistence of Infectious Pancreatic Necrosis Virus and Its Influence on the Early Immune Response","volume":"136","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Mutoloki, S., J\u00f8ssund, T.B., Ritchie, G., Munang\u2019andu, H.M., and Evensen, \u00d8. (2016). Infectious Pancreatic Necrosis Virus Causing Clinical and Subclinical Infections in Atlantic Salmon Have Different Genetic Fingerprints. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01393"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1128\/JVI.01536-09","article-title":"Crystal Structure of an Aquabirnavirus Particle: Insights into Antigenic Diversity and Virulence Determinism","volume":"84","author":"Coulibaly","year":"2010","journal-title":"J. Virol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Klaitong, P., and Smith, D.R. (2021). Roles of Non-Structural Protein 4A in Flavivirus Infection. Viruses, 13.","DOI":"10.3390\/v13102077"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.virol.2022.07.002","article-title":"Influenza Virus NS1 Interacts with 14-3-3\u03b5 to Antagonize the Production of RIG-I-Mediated Type I Interferons","volume":"574","author":"Wang","year":"2022","journal-title":"Virology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1128\/JVI.78.3.1063-1068.2004","article-title":"Herpes Simplex Virus Virion Host Shutoff Protein: Immune Evasion Mediated by a Viral RNase?","volume":"78","author":"Smiley","year":"2004","journal-title":"J. Virol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1126\/science.1222213","article-title":"An Overlapping Protein-Coding Region in Influenza A Virus Segment 3 Modulates the Host Response","volume":"337","author":"Jagger","year":"2012","journal-title":"Science"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1006\/viro.2000.0595","article-title":"VP5, the Nonstructural Polypeptide of Infectious Bursal Disease Virus, Accumulates within the Host Plasma Membrane and Induces Cell Lysis","volume":"277","author":"Lombardo","year":"2000","journal-title":"Virology"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Friedel, C.C., Whisnant, A.W., Djakovic, L., Rutkowski, A.J., Friedl, M.-S., Kluge, M., Williamson, J.C., Sai, S., Vidal, R.O., and Sauer, S. (2021). Dissecting Herpes Simplex Virus 1-Induced Host Shutoff at the RNA Level. J. Virol., 95.","DOI":"10.1128\/JVI.01399-20"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e18311","DOI":"10.7554\/eLife.18311","article-title":"A Systematic View on Influenza Induced Host Shutoff","volume":"5","author":"Tai","year":"2016","journal-title":"eLife"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Gervais, O., Pe\u00f1aloza, C., Gratacap, R., Papadopoulou, A., Beltr\u00e1n, M., Henderson, N.C., Houston, R.D., Hassan, M.A., and Robledo, D. (2023). Understanding Host Response to Infectious Salmon Anaemia Virus in an Atlantic Salmon Cell Line Using Single-Cell RNA Sequencing. BMC Genom., 24.","DOI":"10.1186\/s12864-023-09254-z"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Yang, Z., Luo, W., Huang, Z., Guo, M., He, X., Fan, Z., Wang, Q., Qin, Q., Yang, M., and Lee, X. (2022). Genome-Wide Analysis of Differentially Expressed mRNAs and lncRNAs in Koi Carp Infected with Koi Herpesvirus. Viruses, 14.","DOI":"10.3390\/v14112555"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Woldemariam, N.T., Agafonov, O., Sindre, H., H\u00f8yheim, B., Houston, R.D., Robledo, D., Bron, J.E., and Andreassen, R. (2020). miRNAs Predicted to Regulate Host Anti-Viral Gene Pathways in IPNV-Challenged Atlantic Salmon Fry Are Affected by Viral Load, and Associated with the Major IPN Resistance QTL Genotypes in Late Infection. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.02113"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"738000","DOI":"10.1016\/j.aquaculture.2022.738000","article-title":"RNA-Seq Analysis Reveals the Dynamic Regulation of Proteasomal and Autophagic Degradation Systems of Rainbow Trout (Oncorhynchus Mykiss) Skeletal Muscle Challenged with Infectious Pancreatic Necrosis Virus (IPNV)","volume":"552","author":"Aedo","year":"2022","journal-title":"Aquaculture"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Tapia, D., Kuznar, J., Farlora, R., and Y\u00e1\u00f1ez, J.M. (2022). Differential Transcriptomic Response of Rainbow Trout to Infection with Two Strains of IPNV. Viruses, 14.","DOI":"10.3390\/v14010021"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.fsi.2006.04.002","article-title":"Expression of Mx mRNA Following Infection with IPNV Is Greater in IPN-Susceptible Atlantic Salmon Post-Smolts than in IPN-Resistant Atlantic Salmon Parr","volume":"22","author":"Lockhart","year":"2007","journal-title":"Fish Shellfish Immunol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1016\/j.fsi.2006.05.004","article-title":"Expression Kinetics of Interferon and Interferon-Induced Genes in Atlantic Salmon (Salmo salar) Following Infection with Infectious Pancreatic Necrosis Virus and Infectious Salmon Anaemia Virus","volume":"22","author":"McBeath","year":"2007","journal-title":"Fish Shellfish Immunol."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Robledo, D., Taggart, J.B., Ireland, J.H., McAndrew, B.J., Starkey, W.G., Haley, C.S., Hamilton, A., Guy, D.R., Mota-Velasco, J.C., and Gheyas, A.A. (2016). Gene Expression Comparison of Resistant and Susceptible Atlantic Salmon Fry Challenged with Infectious Pancreatic Necrosis Virus Reveals a Marked Contrast in Immune Response. BMC Genom., 17.","DOI":"10.1186\/s12864-016-2600-y"},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Taylor, R.S., Ruiz Daniels, R., Dobie, R., Naseer, S., Clark, T.C., Henderson, N.C., Boudinot, P., Martin, S.A.M., and Macqueen, D.J. (2022). Single Cell Transcriptomics of Atlantic Salmon (Salmo salar L.) Liver Reveals Cellular Heterogeneity and Immunological Responses to Challenge by Aeromonas salmonicida. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.984799"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.fsi.2014.08.029","article-title":"Induction of Anti-Inflammatory Cytokine Expression by IPNV in Persistent Infection","volume":"41","author":"Montero","year":"2014","journal-title":"Fish Shellfish Immunol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/12\/1245\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T13:53:39Z","timestamp":1764942819000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/12\/1245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,5]]},"references-count":68,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,12]]}},"alternative-id":["pathogens14121245"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14121245","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,5]]}}}