{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:54:28Z","timestamp":1780487668966,"version":"3.54.1"},"reference-count":111,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["UID\/50017\/2025"],"award-info":[{"award-number":["UID\/50017\/2025"]}]},{"name":"FCT\u2014Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","award":["LA\/P\/0094\/2020"],"award-info":[{"award-number":["LA\/P\/0094\/2020"]}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"crossref","award":["2022\/10775-9"],"award-info":[{"award-number":["2022\/10775-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"crossref","award":["2023\/03797-9"],"award-info":[{"award-number":["2023\/03797-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"crossref","award":["301978\/2022-0"],"award-info":[{"award-number":["301978\/2022-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"name":"FCT\u2014Foundation for Science and Technology","award":["2024.05721.RESTART"],"award-info":[{"award-number":["2024.05721.RESTART"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>This study compared the efficacy of three individual lytic phages, PSA_LMAPSA-2T (PSA-2T), PSA_LMAPSA-6F (PSA-6F) and PSA_LMAPSA-7F (PSA-7F) and four phage cocktails (dual and triple combinations) in inactivating Pseudomonas syringae pv. actinidiae. Phages were isolated from kiwifruit leaves and soil samples contaminated with P. syringae pv. actinidiae and characterized by host spectrum, growth parameters, adsorption rate, genomic analysis, inactivation efficiency and viability under variable environmental conditions in orchard environments (temperature, pH and solar radiation). Phage PSA-2T showed the highest in vitro efficacy, achieving a 3.2 log CFU\/mL maximum reduction after 18 h, outperforming PSA-6F and PSA-7F (0.6 and 1.5 log reductions, respectively). Phage cocktails achieved reductions of 1.0\u20132.2 log CFU\/mL, but none exceeded the performance of PSA-2T alone. Phage viability was most affected by high temperature and acidic pH, with PSA-7F showing the greatest sensitivity. Nonetheless, all phages remained stable under typical orchard conditions. Phage PSA-2T significantly reduced P. syringae pv. actinidiae levels (1.5-log CFU\/mL) on artificially contaminated kiwifruit leaves after a single treatment. These results demonstrate the potential of PSA-2T and phage cocktails as sustainable alternatives to copper and antibiotics, warranting further study of repeated treatments and broad-host-range phage formulations for field use.<\/jats:p>","DOI":"10.3390\/pathogens14121247","type":"journal-article","created":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T16:37:02Z","timestamp":1764952622000},"page":"1247","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Three Novel Bacteriophages for the Biocontrol of Pseudomonas syringae pv. actinidiae on Artificially Contaminated Kiwifruit Leaves"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9209-7687","authenticated-orcid":false,"given":"Carla","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Biology and CESAM, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Gomes","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4025-442X","authenticated-orcid":false,"given":"Pedro","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3394-5663","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2510-612X","authenticated-orcid":false,"given":"M\u00e1rcia","family":"Braz","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4223-0851","authenticated-orcid":false,"given":"Vanessa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM, University of Aveiro, Campus Universit\u00e1rio de Santiago, 3810-193 Aveiro, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1934-0091","authenticated-orcid":false,"given":"Newton C. 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