{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T17:18:45Z","timestamp":1765473525325,"version":"3.48.0"},"reference-count":25,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T00:00:00Z","timestamp":1765411200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"West Pomeranian Voivodeship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>High-risk human papillomavirus (HR-HPV) infection with genotypes such as HPV-16 and HPV-18 is a well-established risk factor for cervical cancer; however, its prevalence in oropharyngeal sites among asymptomatic women remains less clearly defined. We evaluated 400 women aged 20\u201340 years (mean age 29.6 years) for the presence of HPV-16, HPV-18, and other HR-HPV genotypes in both cervical and oropharyngeal samples. Cervical specimens were collected using flocked swabs, and oropharyngeal specimens using flocked nylon swabs. Samples were preserved in transport medium, transported at 4\u20138 \u00b0C, and stored at \u201320 \u00b0C until DNA extraction. Viral DNA was isolated with the AA Viral DNA Kit and analyzed using the GenoProf HPV Screening Test, which employs multiplex PCR and reverse hybridization. HPV-16 was detected in 9.5% of participants, HPV-18 in 7.3%, and other HR-HPV genotypes in 14.3%. No statistically significant variation in prevalence across age groups was observed (HPV-16: \u03c72 = 0.10, p = 0.992; HPV-18: \u03c72 = 0.10, p = 0.992; HR-HPV: \u03c72 = 0.15, p = 0.985). Importantly, no HPV DNA was detected in oropharyngeal swabs. These findings indicate that, in this cohort of reproductive-age women, HR-HPV infection was confined to the cervix, with oropharyngeal infection being rare or undetectable. The results underscore the importance of prioritizing cervical screening initiatives in similar populations.<\/jats:p>","DOI":"10.3390\/pathogens14121274","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T17:13:34Z","timestamp":1765473214000},"page":"1274","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Distribution of High-Risk HPV in Cervical Versus Oropharyngeal Samples: Results from a Prospective Screening Study in a Cohort of European Women of Reproductive Age"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2876-5665","authenticated-orcid":false,"given":"Dagmara","family":"Lisman","sequence":"first","affiliation":[{"name":"Department of Genomics and Forensic Genetics, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Ossowski","sequence":"additional","affiliation":[{"name":"Department of Genomics and Forensic Genetics, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0593-0069","authenticated-orcid":false,"given":"Bart\u0142omiej","family":"Grygorcewicz","sequence":"additional","affiliation":[{"name":"Department of Genomics and Forensic Genetics, Pomeranian Medical University in Szczecin, 70-111 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0141ukasz","family":"Tyszler","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Endodontics, Faculty of Dentistry, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafa\u0142","family":"Becht","sequence":"additional","affiliation":[{"name":"Clinical Department of Oncology, Chemotherapy and Cancer Immunotherapy, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1176-2153","authenticated-orcid":false,"given":"Mateusz","family":"Koz\u0142owski","sequence":"additional","affiliation":[{"name":"Department of Reconstructive and Oncological Gynecology, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aneta","family":"Cymbaluk-P\u0142oska","sequence":"additional","affiliation":[{"name":"Department of Reconstructive and Oncological Gynecology, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,11]]},"reference":[{"key":"ref_1","first-page":"209","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. 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