{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T05:59:53Z","timestamp":1789624793840,"version":"build-2803163510"},"reference-count":80,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T00:00:00Z","timestamp":1765497600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"IDEXX Laboratories Inc., Westbrook, ME, USA"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Leishmania (L.) infantum infections in dogs can cause severe recurrent disease. The aim of this study was to investigate different parameters for early detection of disease relapses in L. infantum-infected dogs in Germany. Fifty-two dogs naturally infected with L. infantum were enrolled. During the one-year study period, all dogs remained outside of endemic areas and attended study appointments every three months, including physical examination, blood pressure measurement, complete blood count with differential, serum biochemistry with symmetrical dimethylarginine and C-reactive protein, complete urinalysis including urine protein-to-creatinine ratio, L. infantum PCR, and antibody ELISA. Disease relapse was defined as deterioration of clinical or laboratory parameters in dogs that had achieved complete or partial remission before. Univariable and multivariable Bayesian logistic regression were used to identify predictors of disease relapse. Lymphadenopathy (p &lt; 0.01; OR = 6.93), seborrhea\/hypotrichosis (p = 0.02; OR = 8.02), and proteinuria (p &lt; 0.01; OR = 9.14) were significantly associated with upcoming disease relapses (n = 10; 9\/52 dogs), while associations between higher antibody levels and upcoming disease relapses trended towards significance (p = 0.06; OR = 1.03). Different parameters are important for an early diagnosis of disease relapse in canine leishmaniosis and should thus be regularly assessed and interpreted accordingly in the monitoring of L. infantum-infected dogs.<\/jats:p>","DOI":"10.3390\/pathogens14121282","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T09:36:39Z","timestamp":1765791399000},"page":"1282","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Importance of Different Parameters for Monitoring Dogs with Leishmania infantum Infections in a Non-Endemic Country"],"prefix":"10.3390","volume":"14","author":[{"given":"Melanie","family":"Kaempfle","sequence":"first","affiliation":[{"name":"LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, Ludwig Maximilian University of Munich (LMU Munich), 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7508-5411","authenticated-orcid":false,"given":"Roswitha","family":"Dorsch","sequence":"additional","affiliation":[{"name":"LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, Ludwig Maximilian University of Munich (LMU Munich), 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6928-4089","authenticated-orcid":false,"given":"Yury","family":"Zablotski","sequence":"additional","affiliation":[{"name":"LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, Ludwig Maximilian University of Munich (LMU Munich), 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5256-863X","authenticated-orcid":false,"given":"Katrin","family":"Hartmann","sequence":"additional","affiliation":[{"name":"LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, Ludwig Maximilian University of Munich (LMU Munich), 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mich\u00e8le","family":"Bergmann","sequence":"additional","affiliation":[{"name":"LMU Small Animal Clinic, Centre for Clinical Veterinary Medicine, Ludwig Maximilian University of Munich (LMU Munich), 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cosma, C., Maia, C., Khan, N., Infantino, M., and Del Riccio, M. (2024). Leishmaniasis in Humans and Animals: A one health approach for surveillance, prevention and control in a changing world. Trop. Med. Infect. Dis., 9.","DOI":"10.3390\/tropicalmed9110258"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Lopes, R., Garc\u00eas, A., Silva, A., Brilhante-Sim\u00f5es, P., Martins, \u00c2., Duarte, E.L., Coelho, A.C., and Cardoso, L. (2024). Distribution of and relationships between epidemiological and clinicopathological parameters in canine leishmaniosis: A retrospective study of 15 years (2009\u20132023). Pathogens, 13.","DOI":"10.3390\/pathogens13080635"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"102211","DOI":"10.1016\/j.cimid.2024.102211","article-title":"Canine leishmaniosis global prevalence over the last three decades: A meta-analysis and systematic review","volume":"112","author":"Priolo","year":"2024","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Fischer, D., Moeller, P., Thomas, S.M., Naucke, T.J., and Beierkuhnlein, C. (2011). Combining climatic projections and dispersal ability: A method for estimating the responses of sandfly vector species to climate change. PLoS Negl. Trop. Dis., 5.","DOI":"10.1371\/journal.pntd.0001407"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Morales-Yuste, M., Mart\u00edn-S\u00e1nchez, J., and Corpas-Lopez, V. (2022). Canine leishmaniasis: Update on epidemiology, diagnosis, treatment, and prevention. Vet. Sci., 9.","DOI":"10.3390\/vetsci9080387"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/1756-3305-4-48","article-title":"The immunopathology of canine vector-borne diseases","volume":"4","author":"Day","year":"2011","journal-title":"Parasit. Vectors"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/j.1365-3024.2006.00840.x","article-title":"Immunology of canine leishmaniasis","volume":"28","year":"2006","journal-title":"Parasite Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3296893","DOI":"10.1155\/2018\/3296893","article-title":"Canine leishmaniasis: An overview of the current status and strategies for control","volume":"2018","author":"Ribeiro","year":"2018","journal-title":"Biomed. Res. Int."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Baxarias, M., Jornet-Rius, O., Donato, G., Mateu, C., Alcover, M.M., Pennisi, M.G., and Solano-Gallego, L. (2023). Signalment, immunological and parasitological status and clinicopathological findings of Leishmania-seropositive apparently healthy dogs. Animals, 13.","DOI":"10.3390\/ani13101649"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1177\/0300985814521248","article-title":"Pathologic mechanisms underlying the clinical findings in canine leishmaniosis due to Leishmania infantum\/chagasi","volume":"51","author":"Koutinas","year":"2014","journal-title":"Vet. Pathol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Cacheiro-Llaguno, C., Parody, N., Escutia, M.R., and Carn\u00e9s, J. (2021). Role of circulating immune complexes in the pathogenesis of canine leishmaniasis: New players in vaccine development. Microorganisms, 9.","DOI":"10.3390\/microorganisms9040712"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1111\/j.1365-3164.2009.00823.x","article-title":"Advances in the pathogenesis of canine leishmaniosis: Epidemiologic and diagnostic implications","volume":"20","author":"Saridomichelakis","year":"2009","journal-title":"Vet. Dermatol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Maia, C., and Campino, L. (2018). Biomarkers associated with Leishmania infantum exposure, infection, and disease in dogs. Front. Cell Infect. Microbiol., 8.","DOI":"10.3389\/fcimb.2018.00302"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1128\/JCM.44.4.1318-1322.2006","article-title":"Incidence and time course of Leishmania infantum infections examined by parasitological, serologic, and nested-PCR techniques in a cohort of naive dogs exposed to three consecutive transmission seasons","volume":"44","author":"Oliva","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1186\/1756-3305-4-86","article-title":"LeishVet guidelines for the practical management of canine leishmaniosis","volume":"4","author":"Koutinas","year":"2011","journal-title":"Parasit. Vectors"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.tvjl.2013.04.001","article-title":"Prognosis and monitoring of leishmaniasis in dogs: A working group report","volume":"198","author":"Roura","year":"2013","journal-title":"Vet. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1136\/vr.100637","article-title":"Prognostic analytes in dogs with Leishmania infantum infection living in a non-endemic area","volume":"171","author":"Geisweid","year":"2012","journal-title":"Vet. Rec."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1186\/s13071-016-1519-0","article-title":"Early reduction of Leishmania infantum-specific antibodies and blood parasitemia during treatment in dogs with moderate or severe disease","volume":"9","author":"Ordeix","year":"2016","journal-title":"Parasit. Vectors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/1756-3305-4-52","article-title":"Infectivity to Phlebotomus perniciosus of dogs naturally parasitized with Leishmania infantum after different treatments","volume":"4","author":"Fraile","year":"2011","journal-title":"Parasit. Vectors"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1111\/j.1365-3164.2005.00460.x","article-title":"Treatment of canine Old World visceral leishmaniasis: A systematic review","volume":"16","author":"Noli","year":"2005","journal-title":"Vet. Dermatol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Kaempfle, M., Hartmann, K., and Bergmann, M. (2025). Treatment of Leishmania infantum infections in dogs. Microorganisms, 13.","DOI":"10.3390\/microorganisms13051018"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1007\/s11250-024-03978-0","article-title":"Alternative hosts of Leishmania infantum: A neglected parasite in Europe","volume":"56","author":"Tsakmakidis","year":"2024","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1016\/j.pt.2017.05.005","article-title":"Novel areas for prevention and control of canine leishmaniosis","volume":"33","author":"Petersen","year":"2017","journal-title":"Trends Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sarquis, J., Parody, N., Montoya, A., Cacheiro-Llaguno, C., Barrera, J.P., Checa, R., Daza, M.A., Carn\u00e9s, J., and Mir\u00f3, G. (2024). Clinical validation of circulating immune complexes for use as a diagnostic marker of canine leishmaniosis. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1368929"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1186\/s13071-015-0896-0","article-title":"Long-term follow-up of dogs with leishmaniosis treated with meglumine antimoniate plus allopurinol versus miltefosine plus allopurinol","volume":"8","author":"Manna","year":"2015","journal-title":"Parasit. Vectors"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Mir\u00f3, G., Segarra, S., Cer\u00f3n, J.J., Ferrer, L., Solano-Gallego, L., Montell, L., Costa, E., Teichenne, J., Marin\u00e9-Casad\u00f3, R., and Roura, X. (2024). New immunomodulatory treatment protocol for canine leishmaniosis reduces parasitemia and proteinuria. PLoS Negl. Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0012712"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Pereira, M.A., Santos, R., N\u00f3brega, C., Mega, C., Cruz, R., Esteves, F., Santos, C., Coelho, C., Mesquita, J.R., and Vala, H. (2022). A questionnaire-based survey on the long-term management of canine leishmaniosis by veterinary practitioners. Animals, 12.","DOI":"10.3390\/ani12060731"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1186\/s13071-023-05777-2","article-title":"Survival time and prognostic factors in canine leishmaniosis in a non-endemic country treated with a two-phase protocol including initial allopurinol monotherapy","volume":"16","author":"Rappoldt","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Pereira, M.A., Santos, R., Oliveira, R., Costa, L., Prata, A., Gon\u00e7alves, V., Roquette, M., Vala, H., and Santos-Gomes, G. (2020). Prognostic factors and life expectancy in canine leishmaniosis. Vet. Sci., 7.","DOI":"10.3390\/vetsci7030128"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1590\/0037-8682-0187-2015","article-title":"Predictive factors for Leishmania infantum infection in dogs examined at a veterinary teaching hospital in Teresina, State of Piau\u00ed, Brazil","volume":"49","author":"Costa","year":"2016","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"de Sousa Gon\u00e7alves, R., Alves de Pinho, F., Dinis-Oliveira, R.J., Azevedo, R., Gaifem, J., Larangeira, D.F., Ramos-Sanchez, E.M., Goto, H., Silvestre, R., and Barrouin-Melo, S.M. (2020). Mathematical modelling using predictive biomarkers for the outcome of canine leishmaniasis upon chemotherapy. Microorganisms, 8.","DOI":"10.3390\/microorganisms8050745"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/j.ijpara.2024.03.006","article-title":"Exploring the impact of epidemiological and clinical factors on the progression of canine leishmaniosis by statistical and whole genome analyses: From breed predisposition to comorbidities","volume":"54","author":"Sanz","year":"2024","journal-title":"Int. J. Parasitol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/jsap.13249","article-title":"Canine leishmaniosis and kidney disease: Q&A for an overall management in clinical practice","volume":"62","author":"Roura","year":"2021","journal-title":"J. Small Anim. Pract."},{"key":"ref_34","unstructured":"Budde, J.A., and McCluskey, D.M. (2023). Plumb\u2019s Veterinary Drug Handbook, John Wiley & Sons."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Kaempfle, M., Bergmann, M., Koelle, P., and Hartmann, K. (2023). High performance liquid chromatography analysis and description of purine content of diets suitable for dogs with Leishmania infection during allopurinol treatment\u2014A pilot trial. Animals, 13.","DOI":"10.3390\/ani13193060"},{"key":"ref_36","unstructured":"(2025, October 14). LeishVet Fact Sheet: Practical Management of Canine Leishmaniosis. Available online: https:\/\/www.leishvet.org\/canine-leishmaniosis-guidelines\/."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.tvjl.2016.02.012","article-title":"Infectious diseases in dogs rescued during dogfighting investigations","volume":"211","author":"Cannon","year":"2016","journal-title":"Vet. J."},{"key":"ref_38","unstructured":"Dohoo, I.R., Martin, S.W., and Stryhn, H. (2009). Veterinary Epidemiologic Research, VER, Inc.. [2nd ed.]."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"603","DOI":"10.2307\/2533961","article-title":"Model selection: An integral part of inference","volume":"53","author":"Buckland","year":"1997","journal-title":"Biometrics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1","DOI":"10.18637\/jss.v034.i12","article-title":"glmulti: An R package for easy automated model selection with (generalized) linear models","volume":"34","author":"Calcagno","year":"2010","journal-title":"J. Stat. Softw."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1186\/s13071-024-06423-1","article-title":"Relapses in canine leishmaniosis: Risk factors identified through mixed-effects logistic regression","volume":"17","author":"Sarquis","year":"2024","journal-title":"Parasit. Vectors"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.vetpar.2014.10.035","article-title":"Course of experimental infection of canine leishmaniosis: Follow-up and utility of noninvasive diagnostic techniques","volume":"207","author":"Montoya","year":"2015","journal-title":"Vet. Parasitol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0165-2427(02)00134-4","article-title":"Cytokine expression during the outcome of canine experimental infection by Leishmania infantum","volume":"88","author":"Rosa","year":"2002","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.rvsc.2005.09.011","article-title":"Parasite density and impaired biochemical\/hematological status are associated with severe clinical aspects of canine visceral leishmaniasis","volume":"81","author":"Reis","year":"2006","journal-title":"Res. Vet. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.rvsc.2017.10.011","article-title":"Clinicopathological findings in sick dogs naturally infected with Leishmania infantum: Comparison of five different clinical classification systems","volume":"117","author":"Ordeix","year":"2018","journal-title":"Res. Vet. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/j.tvjl.2014.09.002","article-title":"An update on the diagnosis and treatment of canine leishmaniosis caused by Leishmania infantum (syn. L.\u2009chagasi)","volume":"202","author":"Noli","year":"2014","journal-title":"Vet. J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1136\/vr.141.21.539","article-title":"A retrospective clinical study of canine leishmaniasis in 150 dogs naturally infected by Leishmania infantum","volume":"141","author":"Ciaramella","year":"1997","journal-title":"Vet. Rec."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Foglia Manzillo, V., Di Muccio, T., Cappiello, S., Scalone, A., Paparcone, R., Fiorentino, E., Gizzarelli, M., Gramiccia, M., Gradoni, L., and Oliva, G. (2013). Prospective study on the incidence and progression of clinical signs in na\u00efve dogs naturally infected by Leishmania infantum. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002225"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1186\/1756-3305-7-110","article-title":"Management of canine leishmaniosis in endemic SW European regions: A questionnaire-based multinational survey","volume":"7","author":"Bourdeau","year":"2014","journal-title":"Parasit. Vectors"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.actatropica.2004.04.007","article-title":"Canine visceral leishmaniasis: A histopathological study of lymph nodes","volume":"92","author":"Lima","year":"2004","journal-title":"Acta Trop."},{"key":"ref_51","unstructured":"Sykes, J.E. (2021). 96\u2014Leishmaniosis. Greene\u2019s Infectious Diseases of the Dog and Cat, W.B. Saunders. [5th ed.]."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1111\/j.1939-165X.2005.tb00048.x","article-title":"Cytologic patterns of lymphadenopathy in dogs infected with Leishmania infantum","volume":"34","author":"Mylonakis","year":"2005","journal-title":"Vet. Clin. Pathol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.vetimm.2007.07.009","article-title":"Histopathology, parasite density and cell phenotypes of the popliteal lymph node in canine visceral leishmaniasis","volume":"121","author":"Giunchetti","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1111\/j.1748-5827.1988.tb02298.x","article-title":"Skin lesions in canine leishmaniasis","volume":"29","author":"Ferrer","year":"1988","journal-title":"J. Small Anim. Pract."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0021-9975(03)00063-X","article-title":"Histological and immunohistochemical study of clinically normal skin of Leishmania infantum-infected dogs","volume":"130","author":"Morell","year":"2004","journal-title":"J. Comp. Pathol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1111\/vde.12105","article-title":"Cutaneous involvement in canine leishmaniosis due to Leishmania infantum (syn. L. chagasi)","volume":"25","author":"Saridomichelakis","year":"2014","journal-title":"Vet. Dermatol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1111\/vde.70006","article-title":"World association for veterinary dermatology consensus statement for diagnosis, and evidence-based clinical practice guidelines for treatment and prevention of canine leishmaniosis","volume":"36","author":"Saridomichelakis","year":"2025","journal-title":"Vet. Dermatol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/j.vetimm.2004.12.001","article-title":"Cellular immunophenotyping of exfoliative dermatitis in canine leishmaniosis (Leishmania infantum)","volume":"104","author":"Papadogiannakis","year":"2005","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1186\/s13071-017-2051-6","article-title":"Histological and parasitological distinctive findings in clinically-lesioned and normal-looking skin of dogs with different clinical stages of leishmaniosis","volume":"10","author":"Ordeix","year":"2017","journal-title":"Parasit. Vectors"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1354\/vp.42-2-166","article-title":"Cutaneous leishmaniasis in naturally infected dogs is associated with a T helper-2-biased immune response","volume":"42","author":"Brachelente","year":"2005","journal-title":"Vet. Pathol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/j.vetpar.2006.06.028","article-title":"Leishmania DNA load and cytokine expression levels in asymptomatic naturally infected dogs","volume":"142","author":"Manna","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"3540","DOI":"10.1111\/tbed.14717","article-title":"Pre-test probability and likelihood ratios for clinical findings in canine leishmaniasis","volume":"69","author":"Maldonado","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"558","DOI":"10.2460\/ajvr.2003.64.558","article-title":"Glomerular lesions in dogs infected with Leishmania organisms","volume":"64","author":"Zatelli","year":"2003","journal-title":"Am. J. Vet. Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1111\/vcp.12413","article-title":"Laboratory tests for diagnosing and monitoring canine leishmaniasis","volume":"45","author":"Paltrinieri","year":"2016","journal-title":"Vet. Clin. Pathol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"525","DOI":"10.17236\/sat00362","article-title":"Prognostic factors in dogs with common causes of proteinuria","volume":"164","author":"Baumgartner","year":"2022","journal-title":"Schweiz. Arch. Tierheilkd."},{"key":"ref_66","first-page":"S27","article-title":"Consensus recommendations for standard therapy of glomerular disease in dogs","volume":"27","author":"Brown","year":"2013","journal-title":"J. Vet. Intern. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1186\/s13071-022-05222-w","article-title":"Dogs with leishmaniosis: How are we managing proteinuria in daily practice? A Portuguese questionnaire-based study","volume":"15","author":"Monteiro","year":"2022","journal-title":"Parasit. Vectors"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"235","DOI":"10.2460\/ajvr.71.2.235","article-title":"Evaluation of a urine dipstick test for confirmation or exclusion of proteinuria in dogs","volume":"71","author":"Zatelli","year":"2010","journal-title":"Am. J. Vet. Res."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1186\/s13071-025-06940-7","article-title":"Seasonal variation of anti-Leishmania infantum antibodies and laboratory abnormalities in dogs with leishmaniosis","volume":"18","author":"Cavalera","year":"2025","journal-title":"Parasit. Vectors"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"105638","DOI":"10.1016\/j.tvjl.2021.105638","article-title":"Seasonal variation in canine anti-Leishmania infantum antibody titres","volume":"271","author":"Cavalera","year":"2021","journal-title":"Vet. J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2009.05.022","article-title":"Directions for the diagnosis, clinical staging, treatment and prevention of canine leishmaniosis","volume":"165","author":"Koutinas","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1177\/1040638718824140","article-title":"Serum indirect immunofluorescence assay and real-time PCR results in dogs affected by Leishmania infantum: Evaluation before and after treatment at different clinical stages","volume":"31","author":"Bruno","year":"2019","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1111\/j.1365-3164.2009.00824.x","article-title":"Multicentric, controlled clinical study to evaluate effectiveness and safety of miltefosine and allopurinol for canine leishmaniosis","volume":"20","author":"Oliva","year":"2009","journal-title":"Vet. Dermatol."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Ceron, J.J., Pardo-Marin, L., Caldin, M., Furlanello, T., Solano-Gallego, L., Tecles, F., Bernal, L., Baneth, G., and Martinez-Subiela, S. (2018). Use of acute phase proteins for the clinical assessment and management of canine leishmaniosis: General recommendations. BMC Vet. Res., 14.","DOI":"10.1186\/s12917-018-1524-y"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.tvjl.2007.04.013","article-title":"Real-time PCR assay in Leishmania-infected dogs treated with meglumine antimoniate and allopurinol","volume":"177","author":"Manna","year":"2008","journal-title":"Vet. J."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.vetpar.2006.01.011","article-title":"Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis","volume":"137","author":"Francino","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.tvjl.2013.07.025","article-title":"Evaluation of a conjunctival swab polymerase chain reaction for the detection of Leishmania infantum in dogs in a non-endemic area","volume":"198","author":"Geisweid","year":"2013","journal-title":"Vet. J."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Cavalera, M.A., Zatelli, A., Donghia, R., Mendoza-Roldan, J.A., Gernone, F., Otranto, D., and Iatta, R. (2022). Conjunctival swab real time-PCR in Leishmania infantum seropositive dogs: Diagnostic and prognostic values. Biology, 11.","DOI":"10.3390\/biology11020184"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.vetpar.2011.08.010","article-title":"Detection of Leishmania infantum DNA by real-time PCR in canine oral and conjunctival swabs and comparison with other diagnostic techniques","volume":"184","author":"Lombardo","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.vetpar.2016.06.005","article-title":"Value of the oral swab for the molecular diagnosis of dogs in different stages of infection with Leishmania infantum","volume":"225","author":"Aschar","year":"2016","journal-title":"Vet. 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