{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T01:33:47Z","timestamp":1780882427013,"version":"3.54.1"},"reference-count":51,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Life Sciences \u201cKing Mihai I\u201d"},{"name":"European Commission\u2018s Directorate-General for Health and Food Safety","award":["101200161"],"award-info":[{"award-number":["101200161"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The wolf (Canis lupus) is an apex predator with high mobility and trophic plasticity, serving as a valuable indicator of helminth transmission at the wildlife\u2013livestock interface. Given the ecological overlap between wolves and both wild and domestic ungulates in Romania, we aimed to identify and molecularly characterize cestodes from wolves\u2019 small intestines. Between November 2022 and June 2025, small intestines from nine wolves were collected across four Romanian counties, frozen, and examined using classical parasitology (macroscopic and microscopic) and molecular methods (PCR amplification and Sanger sequencing of mitochondrial cox1, nad1, and 12S rRNA fragments). Taeniids were detected in three (33.33%) out of nine tested individuals. Genetic analyses confirmed the presence of Taenia krabbei and Taenia hydatigena\u2014species not previously reported in wolves from Romania. This study provides the first molecular evidence of T. krabbei and T. hydatigena in wolves from Romania, and likely Eastern Europe, indicating active transmission and underscoring the need for broader surveillance of hosts to clarify their ecology and regional dynamics within a One Health context.<\/jats:p>","DOI":"10.3390\/pathogens15010018","type":"journal-article","created":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T10:57:18Z","timestamp":1766487438000},"page":"18","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Tapeworms in an Apex Predator: First Molecular Identification of Taenia krabbei and Taenia hydatigena in Wolves (Canis lupus) from Romania"],"prefix":"10.3390","volume":"15","author":[{"given":"Maria Monica Florina","family":"Moraru","sequence":"first","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana-Maria","family":"Marin","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8535-3434","authenticated-orcid":false,"given":"Dan-Cornel","family":"Popovici","sequence":"additional","affiliation":[{"name":"Forestry Faculty, Transilvania University Brasov, 500123 Brasov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4102-2447","authenticated-orcid":false,"given":"Azzurra","family":"Santoro","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis (One Health), Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"},{"name":"European Union Reference Laboratory for Parasites (EURL-P), Foodborne and Neglected Parasites Unit, Department of infectious Diseases, Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9183-7591","authenticated-orcid":false,"given":"Adriano","family":"Casulli","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for the Epidemiology, Detection and Control of Cystic and Alveolar Echinococcosis (One Health), Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"},{"name":"European Union Reference Laboratory for Parasites (EURL-P), Foodborne and Neglected Parasites Unit, Department of infectious Diseases, Istituto Superiore di Sanit\u00e0, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8814-1013","authenticated-orcid":false,"given":"Sorin","family":"Morariu","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5484-4685","authenticated-orcid":false,"given":"Marius Stelian","family":"Ilie","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7314-4641","authenticated-orcid":false,"given":"Violeta","family":"Igna","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narcisa","family":"Mederle","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Mihai I\u201d, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,23]]},"reference":[{"key":"ref_1","unstructured":"Sinclair, R.E., and Fryxell, J. 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