{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:22:08Z","timestamp":1790997728282,"version":"4.1.0"},"reference-count":71,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Financier of studies and projects (FINEP), Brazil","award":["01.22.0495.00"],"award-info":[{"award-number":["01.22.0495.00"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil","award":["88887.919409\/2023-00"],"award-info":[{"award-number":["88887.919409\/2023-00"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Leishmania guyanensis is one of 15 American human-pathogenic species, frequently linked to therapeutic failure due to its marked genetic plasticity and adaptability under drug pressure. To broaden the genomic understanding of this species, its biological traits, and potential therapeutic alternatives, we sequenced the L. guyanensis strain MHOM\/BR\/75\/M4147. Raw reads underwent quality-filtering and assembly. Taxonomic classification utilized BLASTn and Kraken2, confirming that 99.95% of contigs matched Leishmania. The assembled genome size was 31 Mb, with an N50 of 4743 bp and 40.85\u00d7 coverage. Variant calling subsequently identified 36,665 SNPs, 8210 indels, and chromosomal aneuploidies. Genomic annotation identified 3119 proteins with known molecular functions in L. guyanensis, alongside 6371 orthologous genes shared with L. major and L. panamensis. The search for pharmacological relevance yielded ten candidate genes, including one calpain and nine GSK3 family members. Phylogenetic reconstruction using the polA1 gene consistently grouped L. guyanensis, demonstrating strong discriminatory capacity, with L. martiniquensis emerging as the most divergent species. Overall, these findings expand the available genomic framework for L. guyanensis and support advances in species-specific diagnostic approaches.<\/jats:p>","DOI":"10.3390\/pathogens15010124","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T09:16:59Z","timestamp":1769159819000},"page":"124","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Resequencing and De Novo Assembly of Leishmania (Viannia) guyanensis from Amazon Region: Genome Assessment, Phylogenetic Insights and Therapeutic Targets"],"prefix":"10.3390","volume":"15","author":[{"given":"Lucas George Assun\u00e7\u00e3o","family":"Costa","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia das Leishmanioses, Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Rodovia BR 316, km 07, s\/n, Ananindeua 67.030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edivaldo Costa","family":"Sousa Junior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia das Leishmanioses, Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Rodovia BR 316, km 07, s\/n, Ananindeua 67.030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Cristina","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia das Leishmanioses, Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Rodovia BR 316, km 07, s\/n, Ananindeua 67.030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Millena Arnaud Franco da","family":"Igreja","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia das Leishmanioses, Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Rodovia BR 316, km 07, s\/n, Ananindeua 67.030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1780-3729","authenticated-orcid":false,"given":"Franklyn Samudio","family":"Acosta","sequence":"additional","affiliation":[{"name":"Instituto Conmemorativo Gorgas de Estudios de la Salud, Avenida Justo Arosemena, Panama City P.O. Box 0816-02593, Panama"},{"name":"Facultad de Ciencias Naturales Exactas y Tecnolog\u00eda, Universidad de Panam\u00e1, Panama City P.O. Box 0824-00073, Panama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8169-0610","authenticated-orcid":false,"given":"Fabiano Reis da","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia das Leishmanioses, Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Rodovia BR 316, km 07, s\/n, Ananindeua 67.030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes Maria","family":"Garcez","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia das Leishmanioses, Se\u00e7\u00e3o de Parasitologia, Instituto Evandro Chagas, Rodovia BR 316, km 07, s\/n, Ananindeua 67.030-000, PA, Brazil"},{"name":"Departamento de Patologia, Centro de Ci\u00eancias Biol\u00f3gicas e da Sa\u00fade, Universidade do Estado do Par\u00e1, Travessa Perebebui, 2623, Bel\u00e9m 66.087-662, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1007\/s13744-018-0587-6","article-title":"Morphological Description of the Immature Stages of Nyssomyia umbratilis (Ward & Frahia) (Diptera: Psychodidae: Phlebotominae), the Main Vector of Leishmania guyanensis Floch (Kinetoplastida: Trypanosomatidae) in the Brazilian Amazon Region","volume":"47","author":"Alencar","year":"2018","journal-title":"Neotrop. 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