{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T06:03:17Z","timestamp":1785304997354,"version":"3.55.0"},"reference-count":51,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Research & Development Program of Ningxia Province","award":["2022BEG03112"],"award-info":[{"award-number":["2022BEG03112"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32560041"],"award-info":[{"award-number":["32560041"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia Province","doi-asserted-by":"crossref","award":["2024AAC03512"],"award-info":[{"award-number":["2024AAC03512"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Minute virus of canines (MVC) is an autonomous canine parvovirus that causes severe pathological outcomes, including embryo mortality, spontaneous abortion, and congenital malformations in neonatal puppies. Although MVC infection has been shown to induce host cell cycle arrest and apoptosis, the underlying regulatory mechanisms that coordinate cell proliferation and control apoptotic responses during viral replication remain poorly understood. Sirtuin 1 (SIRT1) is an NAD+-dependent deacetylase that plays a critical role in regulating cell cycle progression, DNA damage responses, and apoptosis. However, its involvement in MVC infection has not been fully elucidated. Herein, we show that MVC infection markedly upregulates the mRNA and protein expression levels of SIRT1 in a time-dependent manner. MVC infection activates the SIRT1-p53 signaling axis and modulates the acetylation status of p53. In addition, MVC alters the subcellular distribution of SIRT1, promoting its nuclear translocation and colocalization with the viral protein VP2. Functional analyses demonstrated that pharmacological activation of SIRT1 enhanced the viability of MVC-infected WRD cells (virus-tropic cell), promoting viral replication, prolonging S-phase arrest, and reducing apoptosis. Conversely, inhibition of SIRT1 produced the opposite effects, which were closely associated with regulation of the SIRT1-p53 signaling axis. Moreover, SIRT1 knockdown accelerated apoptosis and attenuated S-phase arrest, whereas SIRT1 overexpression further strengthened S-phase retention. Collectively, our findings identify the SIRT1-p53 signaling axis as an important regulator of cell cycle progression and apoptosis during MVC infection, highlighting SIRT1 as a key host factor that supports viral replication and persistence and a potential target for antiviral intervention.<\/jats:p>","DOI":"10.3390\/pathogens15030242","type":"journal-article","created":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T08:50:27Z","timestamp":1772009427000},"page":"242","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The SIRT1-Mediated p53 Deacetylation Pathway Modulates Apoptosis and Promotes Viral Replication in MVC-Infected Cells"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5892-9992","authenticated-orcid":false,"given":"Yan","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China"},{"name":"Ningxia Institute of Clinical Medicine, People\u2019s Hospital of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan 750002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9603-5591","authenticated-orcid":false,"given":"Xiang","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China"},{"name":"Peking University First Hospital Ningxia Women and Children\u2019s Hospital (Ningxia Hui Autonomous Region Maternal and Child Health Hospital), Yinchuan 750001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yishu","family":"Xiao","sequence":"additional","affiliation":[{"name":"The Third Clinical Medical College of Ningxia Medical University, People\u2019s Hospital of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan 750002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3155-662X","authenticated-orcid":false,"given":"Zhiping","family":"Hei","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8867-8462","authenticated-orcid":false,"given":"Yuning","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1128\/iai.1.5.503-508.1970","article-title":"Recovery and characterization of a minute virus of canines","volume":"1","author":"Binn","year":"1970","journal-title":"Infect. 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