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Unlike conventional molecular assays, LAMP does not require sophisticated instrumentation or highly specialized personnel, rendering it particularly suitable for diagnostic deployment in resource-limited settings. Reaction outcomes are typically determined through direct visual inspection, often via colorimetric readouts, further enhancing its applicability in decentralized and point-of-care contexts. Owing to these attributes, LAMP has emerged as a valuable tool for the diagnosis of infectious diseases, particularly in regions with constrained laboratory infrastructure. Its affordability, rapid turnaround time, and ease of implementation support large-scale testing during public health emergencies, including epidemics and outbreaks, thereby contributing to the reduction in disease burden. Timely and accurate pathogen detection using LAMP can substantially strengthen public health responses aimed at controlling and mitigating viral transmission. This review provides an overview of the LAMP methodology, with an emphasis on its application in the detection of viral pathogens with epidemic and pandemic potential. Dengue virus and influenza virus are discussed as representative model infections to illustrate the diagnostic performance and practical advantages of LAMP-based assays. In addition, we explore current challenges and future perspectives for the implementation of LAMP in resource-limited settings, highlighting the need for continued technological refinement and contextual adaptation to maximize its impact on global health initiatives.<\/jats:p>","DOI":"10.3390\/pathogens15030248","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T10:33:02Z","timestamp":1772101982000},"page":"248","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of High-Burden Viral Diseases in Resource-Limited Countries"],"prefix":"10.3390","volume":"15","author":[{"given":"Ana Catharina","family":"Vasconcelos-Martins","sequence":"first","affiliation":[{"name":"Center for Viral Surveillance and Serological Assessment (CeVIVAS), Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"},{"name":"Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5849-7326","authenticated-orcid":false,"given":"Marta","family":"Giovanetti","sequence":"additional","affiliation":[{"name":"Department of Sciences and Technologies for Sustainable Development and One Health, Universit\u00e0 Campus Bio-Medico di Roma, 00128 Rome, Italy"},{"name":"Instituto Ren\u00e9 Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte 30190-002, Brazil"},{"name":"Climate Amplified Diseases and Epidemics (CLIMADE) Americas, Belo Horizonte 30190-002, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0501-8280","authenticated-orcid":false,"given":"Maria Carolina","family":"Elias","sequence":"additional","affiliation":[{"name":"Center for Viral Surveillance and Serological Assessment (CeVIVAS), Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"},{"name":"Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0805-6140","authenticated-orcid":false,"given":"Svetoslav Nanev","family":"Slavov","sequence":"additional","affiliation":[{"name":"Center for Viral Surveillance and Serological Assessment (CeVIVAS), Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"},{"name":"Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra Coccuzzo","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Center for Viral Surveillance and Serological Assessment (CeVIVAS), Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"},{"name":"Instituto Butantan, S\u00e3o Paulo 05503-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Notomi, T., Okayama, H., Masubuchi, H., Yonekawa, T., Watanabe, K., Amino, N., and Hase, T. 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