{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:00:52Z","timestamp":1787029252505,"version":"build-2736575974"},"reference-count":72,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Modern Agricultural Industrial System Project","award":["CARS-39-16"],"award-info":[{"award-number":["CARS-39-16"]}]},{"name":"Qinghai Province \u201cKunlun Talents High-End Innovation and Entrepreneurial Talents\u201d Top Talent Project","award":["2024"],"award-info":[{"award-number":["2024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>As a core pastoral region of the Qinghai\u2013Tibet Plateau, Qinghai Province faces substantial threats to livestock production from tick-borne diseases. This study aimed to investigate the prevalence of six bacterial pathogens in dominant tick species from Qinghai Province, to provide baseline epidemiological data for local tick-borne disease surveillance. A total of 1025 questing ticks were collected from key pastoral regions of Qinghai Province during April to May in 2024 and 2025. All ticks were morphologically identified as belonging to 1 family (Ixodidae), 2 genera, and 4 species. Dermacentor nuttalli was the dominant species with a relative dominance of 66.83% (685\/1025, 95% CI: 63.92\u201369.61%), followed by Haemaphysalis qinghaiensis at 30.83% (316\/1025, 95% CI: 28.11\u201333.69%), Dermacentor silvarum at 1.95% (20\/1025, 95% CI: 1.27\u20132.98%), and Dermacentor niveus at 0.39% (4\/1025, 95% CI: 0.15\u20131.01%). PCR detection was performed for six target pathogens, with an overall Brucella spp. DNA detection rate of 0.78% (8\/1025, 95% CI: 0.40\u20131.53%) and an overall Rickettsia spp. detection rate of 16.29% (167\/1025, 95% CI: 14.16\u201318.67%). Statistical analysis showed that the prevalence of Brucella spp. and Rickettsia spp. differed significantly between the two dominant tick species (Fisher\u2019s exact test\/\u03c72 test, all p &lt; 0.001). No Brucella or Rickettsia pathogens were detected in D. silvarum and D. niveus. Notably, detection of Brucella spp. DNA does not confirm the presence of viable bacteria or tick vector competence. This study fills the regional data gap of tick-borne pathogens in Qinghai, and provides reference for the prevention and control of local tick-borne zoonotic diseases.<\/jats:p>","DOI":"10.3390\/pathogens15030305","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T09:00:37Z","timestamp":1773219637000},"page":"305","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Detection and Identification of Bacterial Pathogens in Qinghai Province, China"],"prefix":"10.3390","volume":"15","author":[{"given":"Didi","family":"Zhang","sequence":"first","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, College of Agriculture and Animal Husbandry, Academy of Animal Sciences and Veterinary, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yihong","family":"Ma","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, College of Agriculture and Animal Husbandry, Academy of Animal Sciences and Veterinary, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaixing","family":"Yang","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, College of Agriculture and Animal Husbandry, Academy of Animal Sciences and Veterinary, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuping","family":"Li","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, College of Agriculture and Animal Husbandry, Academy of Animal Sciences and Veterinary, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghua","family":"Wang","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, College of Agriculture and Animal Husbandry, Academy of Animal Sciences and Veterinary, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Hu","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, College of Agriculture and Animal Husbandry, Academy of Animal Sciences and Veterinary, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenghua","family":"Tang","sequence":"additional","affiliation":[{"name":"Qinghai Haidong Center for Animal Disease Prevention and Control, Haidong 810600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Li","sequence":"additional","affiliation":[{"name":"Animal Husbandry and Veterinary Station of Minhe County, Haidong 810800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhen","family":"Li","sequence":"additional","affiliation":[{"name":"Animal Husbandry and Veterinary Station of Menyuan County, Haibei Tibetan Autonomous Prefecture 810399, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5696-2546","authenticated-orcid":false,"given":"Yingna","family":"Jian","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, College of Agriculture and Animal Husbandry, Academy of Animal Sciences and Veterinary, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0977-0493","authenticated-orcid":false,"given":"Liqing","family":"Ma","sequence":"additional","affiliation":[{"name":"Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Diseases and Green Technical Research for Prevention and Control, College of Agriculture and Animal Husbandry, Academy of Animal Sciences and Veterinary, Qinghai University, Xining 810016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Shah, T., Li, Q., Wang, B., Baloch, Z., and Xia, X. 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