{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:22:12Z","timestamp":1785424932040,"version":"3.56.0"},"reference-count":53,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:00:00Z","timestamp":1774656000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007045","name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["CBF-2023-2024-4199"],"award-info":[{"award-number":["CBF-2023-2024-4199"]}],"id":[{"id":"10.13039\/501100007045","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007045","name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["CBF-2025-I-3383"],"award-info":[{"award-number":["CBF-2025-I-3383"]}],"id":[{"id":"10.13039\/501100007045","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CBF-2023-2024-4199"],"award-info":[{"award-number":["CBF-2023-2024-4199"]}],"id":[{"id":"https:\/\/ror.org\/059ex5q34","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CBF-2025-I-3383"],"award-info":[{"award-number":["CBF-2025-I-3383"]}],"id":[{"id":"https:\/\/ror.org\/059ex5q34","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Dengue virus (DENV), an important mosquito-borne orthoflavivirus, represents a growing global threat due to its geographic expansion and recent outbreaks worldwide. In resource-limited endemic settings, the development of affordable diagnostic assays is needed. In this study, we developed and validated a colorimetric reverse transcription loop-mediated isothermal amplification assay (RT-LAMP) for the detection of DENV type 3 (DENV-3) using 95 previously diagnosed clinical samples from Southeastern Mexico. Primers targeting the 3\u2032 untranslated region (3\u2032 UTR) of DENV-3 were designed, and assay conditions were standardized. The colorimetric RT-LAMP DENV-3 system achieved a preliminary limit of detection of 1 \u00d7 103 copies per reaction, with 90.7% sensitivity and 100% specificity. The colorimetric format enabled visual readout without specialized equipment, supporting its potential applicability in point-of-care and resource-limited settings. The developed colorimetric RT-LAMP detection for DENV-3 is intended as a rapid screening\/triage tool that can trigger confirmatory testing or public-health actions.<\/jats:p>","DOI":"10.3390\/pathogens15040359","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T12:11:59Z","timestamp":1774872719000},"page":"359","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection of Dengue Virus Serotype 3 Using a Colorimetric Reverse Transcription Loop-Mediated Isothermal Amplification Assay: Evaluation with Clinical Samples from Southeastern Mexico"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2334-9447","authenticated-orcid":false,"given":"Perla","family":"P\u00e9rez-Tepos","sequence":"first","affiliation":[{"name":"Unidad de Investigaci\u00f3n Biom\u00e9dica Oncol\u00f3gica Gen\u00f3mica, Hospital Gineco Pediatr\u00eda 3A, OOAD Cd Mx Norte, Instituto Mexicano del Seguro Social, Ciudad de Mexico 07760, Mexico"},{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Ciudad de Mexico 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7112-1489","authenticated-orcid":false,"given":"Gilma Guadalupe","family":"S\u00e1nchez-Burgos","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica Yucat\u00e1n, Instituto Mexicano del Seguro Social, M\u00e9rida 97150, Yucat\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7366-7725","authenticated-orcid":false,"given":"Beatriz","family":"Xoconostle-C\u00e1zares","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Ciudad de Mexico 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3961-6449","authenticated-orcid":false,"given":"Gloria Mar\u00eda","family":"Molina-Salinas","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n M\u00e9dica Yucat\u00e1n, Instituto Mexicano del Seguro Social, M\u00e9rida 97150, Yucat\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Huch\u00edn-Cetz","sequence":"additional","affiliation":[{"name":"Laboratorio de Apoyo a la Vigilancia Epidemiol\u00f3gica, Unidad de Investigaci\u00f3n M\u00e9dica Yucat\u00e1n, Instituto Mexicano del Seguro Social, M\u00e9rida 97150, Yucat\u00e1n, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7047-0222","authenticated-orcid":false,"given":"Edgar","family":"Sevilla-Reyes","sequence":"additional","affiliation":[{"name":"Laboratorio de Transcript\u00f3mica e Inmunolog\u00eda Molecular, Instituto Nacional de Enfermedades Respiratorias \u201cIsmael Cos\u00edo Villegas\u201d, Ciudad de Mexico 14080, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6144-1411","authenticated-orcid":false,"given":"Berenice","family":"Calder\u00f3n-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Ciudad de Mexico 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0223-7776","authenticated-orcid":false,"given":"Roberto","family":"Ruiz-Medrano","sequence":"additional","affiliation":[{"name":"Departamento de Biotecnolog\u00eda y Bioingenier\u00eda, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional, Ciudad de Mexico 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6812-9471","authenticated-orcid":false,"given":"Rosalia","family":"Lira","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n Biom\u00e9dica Oncol\u00f3gica Gen\u00f3mica, Hospital Gineco Pediatr\u00eda 3A, OOAD Cd Mx Norte, Instituto Mexicano del Seguro Social, Ciudad de Mexico 07760, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/j.ijid.2023.12.004","article-title":"Climate change and the rising incidence of vector-borne diseases globally","volume":"139","author":"George","year":"2024","journal-title":"Int. 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