{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T19:15:14Z","timestamp":1792523714431,"version":"4.5.0"},"reference-count":77,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T00:00:00Z","timestamp":1775088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Rio de Janeiro State Research Support Foundation","award":["E-26\/210.041\/2024"],"award-info":[{"award-number":["E-26\/210.041\/2024"]}]},{"name":"Young Scientist of Our State Grant","award":["E-26\/204.492\/2024"],"award-info":[{"award-number":["E-26\/204.492\/2024"]}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["312037\/2022-8"],"award-info":[{"award-number":["312037\/2022-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"award":["312037\/2022-8"],"award-info":[{"award-number":["312037\/2022-8"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Babesia vogeli is considered endemic in urban settings of Brazil, whereas Hepatozoon canis remains insufficiently documented in several regions, including the metropolitan area of Rio de Janeiro. This study investigated the frequency, spatial distribution, and determinants of infection by piroplasmids and Hepatozoon spp. in dogs from distinct environments. A total of 372 blood samples from pet dogs were collected between June and October 2023 in Maric\u00e1 (Area 1; n = 105) and in the Administrative Regions of Barra da Tijuca, Guaratiba, and Jacarepagu\u00e1 (Area 2; n = 267). Molecular screening was performed using 18S rRNA gene-based PCR assays, followed by sequencing and phylogenetic inference. Conventional PCR was used for piroplasmids, while both conventional and nested PCR were applied for Hepatozoon detection. Overall, 30 dog samples (8.1%) tested positive. Piroplasmids were detected in 3.5% of dogs, with a higher occurrence in Area 1, whereas Hepatozoon spp. infection was identified in 5.4% of samples, with co-positivity with piroplasmids being rare. All piroplasmid sequences corresponded to B. vogeli, while H. canis was confirmed in thirteen dogs. The absence of owner-reported tick-borne disease history was the main factor associated with hemoparasite positivity. These findings provide the first molecular epidemiological evidence of H. canis circulation in different areas of Rio de Janeiro and highlight the need for integrated diagnostics, surveillance, and targeted actions to improve control.<\/jats:p>","DOI":"10.3390\/pathogens15040383","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T14:09:54Z","timestamp":1775138994000},"page":"383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Epidemiology of Babesia vogeli and Hepatozoon canis in Dogs from Urban and Peri-Urban Areas of Rio de Janeiro, Brazil"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7788-7159","authenticated-orcid":false,"given":"Mariana Santos","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia e Parasitologia, Instituto Biom\u00e9dico, Universidade Federal Fluminense, Rua Outeiro de S\u00e3o Jo\u00e3o Batista, s\/n, Centro, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7716-2743","authenticated-orcid":false,"given":"Jo\u00e3o Pedro Siqueira","family":"Palmer","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Parasitologia, Instituto Biom\u00e9dico, Universidade Federal Fluminense, Rua Outeiro de S\u00e3o Jo\u00e3o Batista, s\/n, Centro, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9694-9453","authenticated-orcid":false,"given":"La\u00eds Verdan","family":"Dib","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Parasitologia, Instituto Biom\u00e9dico, Universidade Federal Fluminense, Rua Outeiro de S\u00e3o Jo\u00e3o Batista, s\/n, Centro, Niter\u00f3i 24020-141, RJ, Brazil"},{"name":"Parasitologia, Faculdade de Medicina de Campos, Alberto Torres Avenue, 217, Centro, Campos dos Goytacazes 28035-581, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4176-5224","authenticated-orcid":false,"given":"Camila Souza Carvalho","family":"Class","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Parasitologia, Instituto Biom\u00e9dico, Universidade Federal Fluminense, Rua Outeiro de S\u00e3o Jo\u00e3o Batista, s\/n, Centro, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0965-779X","authenticated-orcid":false,"given":"Lucas Fernandes","family":"Lob\u00e3o","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Parasitologia, Instituto Biom\u00e9dico, Universidade Federal Fluminense, Rua Outeiro de S\u00e3o Jo\u00e3o Batista, s\/n, Centro, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4560-1339","authenticated-orcid":false,"given":"Fabiana Batalha","family":"Knackfuss","sequence":"additional","affiliation":[{"name":"Zootecnia e Estat\u00edstica, Universidade do Grande Rio, Rua Professor Jos\u00e9 de Souza Herdy, 1160, Jardim Vinte e Cinco de Agosto, Duque de Caxias 25071-202, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5007-1339","authenticated-orcid":false,"given":"Alynne da Silva","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia e Parasitologia, Instituto Biom\u00e9dico, Universidade Federal Fluminense, Rua Outeiro de S\u00e3o Jo\u00e3o Batista, s\/n, Centro, Niter\u00f3i 24020-141, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.2478\/acve-2018-0011","article-title":"Canine babesiosis: Where do we stand?","volume":"68","author":"Petra","year":"2018","journal-title":"Acta Vet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1590\/S1984-29612011000300002","article-title":"Brazilian canine Hepatozoonosis","volume":"20","year":"2011","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1016\/S0195-5616(03)00039-1","article-title":"Canine Babesiosis","volume":"33","author":"Boozer","year":"2003","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"94","DOI":"10.4103\/2229-5070.162490","article-title":"Morphology, epidemiology, and phylogeny of Babesia: An overview","volume":"5","author":"Laha","year":"2015","journal-title":"Trop. Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.vetpar.2011.03.024","article-title":"Detection and molecular characterization of a canine piroplasm from Brazil","volume":"180","author":"Soares","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ren\u00e9-Martellet, M., Moro, C.V., Ch\u00eane, J., Bourdoiseau, G., Chabanne, L., and Mavingui, P. (2015). Update on epidemiology of canine babesiosis in southern France. BMC Vet. Res., 11.","DOI":"10.1186\/s12917-015-0525-3"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1007\/BF00931839","article-title":"Characterization and comparison of merozoite antigens of different Babesia canis isolates by serological and immunological investigations","volume":"81","author":"Hauschild","year":"1995","journal-title":"Parasitol. Res."},{"key":"ref_8","first-page":"100417","article-title":"Canine Babesiosis: A literature review of prevalence, distribution, and diagnosis in Latin America and the Caribbean","volume":"21","year":"2020","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.vetpar.2006.07.030","article-title":"Canine babesiosis: A brazilian perspective","volume":"141","author":"Figueredo","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"565","DOI":"10.2307\/3283781","article-title":"The genus Hepatozoon (Apicomplexa: Adeleina)","volume":"82","author":"Smith","year":"1996","journal-title":"J. Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1016\/j.cvsm.2022.06.011","article-title":"Hepatozoonosis of dogs and cats","volume":"52","author":"Baneth","year":"2022","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0304-4017(00)00378-2","article-title":"Hepatozoon canis infection associated with dog ticks of rural areas of Rio de Janeiro State, Brazil","volume":"94","author":"Massard","year":"2001","journal-title":"Vet. Parasitol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1548","DOI":"10.1007\/s11686-021-00426-z","article-title":"Microscopic detection, hematological evaluation and molecular characterization of piroplasms from naturally infected dogs in Rio de Janeiro, Brazil","volume":"66","author":"Palmer","year":"2021","journal-title":"Acta Parasitol."},{"key":"ref_14","unstructured":"IBGE\u2014Instituto Brasileiro de Geografia e Estat\u00edstica (2025, November 02). Maric\u00e1 (RJ)\u2014Cidades e Estados, Available online: https:\/\/www.ibge.gov.br\/cidades-e-estados\/rj\/marica.html."},{"key":"ref_15","unstructured":"(2025, November 02). Atlas do Desenvolvimento Humano no Brasil. Available online: http:\/\/www.atlasbrasil.org.br."},{"key":"ref_16","unstructured":"(2025, November 02). Meu Munic\u00edpio. Available online: https:\/\/meumunicipio.org.br\/perfil-municipio\/3302700-marica-rj."},{"key":"ref_17","unstructured":"(2025, November 21). Rodrigues, T.d.S.S.R. Maric\u00e1, Transforma\u00e7\u00f5es Urbanas de Um Munic\u00edpio Petro-Rentista. Revista Continentes (UFRRJ). Available online: https:\/\/revistacontinentes.com.br\/index.php\/continentes\/article\/view\/442."},{"key":"ref_18","unstructured":"(2025, November 20). Barra Da Tijuca\u2014Citypopulation. Available online: https:\/\/www.citypopulation.de\/en\/brazil\/rio\/_\/33045570529__barra_da_tijuca\/."},{"key":"ref_19","unstructured":"IBGE\u2014Instituto Brasileiro de Geografia e Estat\u00edstica (2026, January 31). Censo Demogr\u00e1fico 2022: Popula\u00e7\u00e3o e Domic\u00edlios Por Bairros (Dados Preliminares). Available online: https:\/\/www.data.rio\/items\/fd354740f1934bf5bf8e9b0e2b509aa9."},{"key":"ref_20","unstructured":"IBGE\u2014Instituto Brasileiro de Geografia e Estat\u00edstica (2026, January 31). Censo Demogr\u00e1fico 2022: Popula\u00e7\u00e3o e Domic\u00edlios\u2014Resultados Do Universo, Available online: https:\/\/censo2022.ibge.gov.br\/panorama\/."},{"key":"ref_21","unstructured":"(2025, November 21). Pr\u00f3lotes\u2014Parque Estadual Da Pedra Branca, o Maior Do Mundo. Available online: https:\/\/prolotes.com.br\/parque-estadual-da-pedra-branca-o-maior-do-mundo\/."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1590\/s1413-41522017132701","article-title":"A implanta\u00e7\u00e3o do parque natural municipal de Marapendi, no Rio de Janeiro","volume":"22","author":"Poian","year":"2017","journal-title":"Eng. Sanit. Ambient."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"69","DOI":"10.6008\/ESS1983-8344.2012.001.0007","article-title":"Os desafios do planejamento e conserva\u00e7\u00e3o ambiental da Reserva Biol\u00f3gica de Guaratiba (RJ)","volume":"5","author":"Castro","year":"2012","journal-title":"Nat. Conserv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4172","DOI":"10.1128\/JCM.41.9.4172-4177.2003","article-title":"Development and evaluation of a seminested PCR for detection and differentiation of Babesia gibsoni (asian genotype) and B. canis DNA in canine blood samples","volume":"41","author":"Birkenheuer","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1645\/GE-464R.1","article-title":"New molecular data on mammalian Hepatozoon species (Apicomplexa: Adeleorina) from Brazil and Spain","volume":"92","author":"Ruas","year":"2006","journal-title":"J. Parasitol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1645\/0022-3395(2000)086[0366:PROHAA]2.0.CO;2","article-title":"Phylogenetic relationships of Hepatozoon (Apicomplexa: Adeleorina) based on molecular, morphologic, and life-cycle characters","volume":"86","author":"Mathew","year":"2000","journal-title":"J. Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1645\/GE-204R","article-title":"High Prevalence of Hepatozoon spp. (Apicomplexa, Hepatozoidae) infection in water pythons (Liasis fuscus) from tropical Australia","volume":"90","author":"Ujvari","year":"2004","journal-title":"J. Parasitol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1007\/s11686-024-00843-w","article-title":"Piroplasmid infections among domestic dogs in the mountain city of Rio de Janeiro, Brazil","volume":"69","author":"Palmer","year":"2024","journal-title":"Acta Parasitol."},{"key":"ref_29","first-page":"95","article-title":"BioEdit: A User-Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symp. Ser."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"W78","DOI":"10.1093\/nar\/gkae268","article-title":"Interactive Tree of Life (iTOL) v6: Recent updates to the phylogenetic tree display and annotation tool","volume":"52","author":"Letunic","year":"2024","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Palmer, J.P., Gaz\u00eata, G., Andr\u00e9, M., Coelho, A., Corr\u00eaa, L., Damasceno, J., Israel, C., Pereira, R., and Barbosa, A. (2022). Piroplasm Infection in Domestic Cats in the Mountainous Region of Rio de Janeiro, Brazil. Pathogens, 11.","DOI":"10.3390\/pathogens11080900"},{"key":"ref_33","first-page":"238","article-title":"Freq\u00fc\u00eancia de c\u00e3es infectados por Babesia spp. em Campos dos Goytacazes, RJ","volume":"9","author":"Miranda","year":"2008","journal-title":"Ci\u00ean. Anim. Bras."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1590\/S1984-29612012000200012","article-title":"Detection and molecular characterization of piroplasms species from naturally infected dogs in southeast Brazil","volume":"21","author":"Lemos","year":"2012","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_35","first-page":"63","article-title":"Altera\u00e7\u00f5es cl\u00ednico-hematol\u00f3gicas da infec\u00e7\u00e3o por Babesia canis vogeli em c\u00e3es do munic\u00edpio de Serop\u00e9dica, Rio de Janeiro, Brasil","volume":"35","author":"Vilela","year":"2013","journal-title":"Ver. Bras. Med. Vet."},{"key":"ref_36","first-page":"160","article-title":"Molecular epidemiology of Babesia vogeli in dogs from the southeastern region of Rio de Janeiro, Brazil","volume":"13","author":"Paulino","year":"2018","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e032120","DOI":"10.29374\/2527-2179.bjvm032120","article-title":"Molecular Detection and hematological changes in dogs naturally infected by Babesia vogeli in metropolitan region of Rio de Janeiro","volume":"42","author":"Assad","year":"2020","journal-title":"Braz. J. Vet. Med."},{"key":"ref_38","first-page":"178","article-title":"Estudo retrospectivo (1991\u20132005), dos casos de babesiose canina na cidade de Salvador e regi\u00e3o metropolitana, Bahia","volume":"8","author":"Bittencourt","year":"2007","journal-title":"Rev. Bras. Sa\u00fade Prod. Anim."},{"key":"ref_39","first-page":"73","article-title":"Hepatozoonose canina em Campos Dos Goytacazes, RJ","volume":"11","author":"Miranda","year":"2008","journal-title":"Arq. Ci\u00eanc. Vet. Zool. Unipar"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.pt.2012.07.003","article-title":"Ticks and Tick-borne diseases: A one health perspective","volume":"28","author":"Chomel","year":"2012","journal-title":"Trend. Parasitol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e021321","DOI":"10.1590\/s1984-29612022010","article-title":"Occurrence of tick-borne pathogens in dogs in a coastal region of the state of Cear\u00e1, Northeastern Brazil","volume":"31","author":"Oliveira","year":"2022","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1007\/s00436-024-08147-8","article-title":"New Insights on the phylogeography of Hepatozoon canis in Brazil","volume":"123","author":"Fernandes","year":"2024","journal-title":"Parasitol. Res."},{"key":"ref_43","first-page":"4","article-title":"first molecular evidence of Hepatozoon canis in domestic dogs and ticks in fragmented rainforest areas in Mexico","volume":"6","year":"2016","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1007\/s00442-009-1430-x","article-title":"Altitudinal Patterns of Tick and Host Abundance: A potential role for climate change in regulating tick-borne diseases?","volume":"162","author":"Gilbert","year":"2010","journal-title":"Oecologia"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.vetpar.2009.09.011","article-title":"To What Extent has climate change contributed to the recent epidemiology of tick-borne diseases?","volume":"167","author":"Randolph","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1016\/j.ijppaw.2015.07.001","article-title":"Climate change, biodiversity, ticks and tick-borne diseases: The butterfly effect","volume":"4","year":"2015","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez P\u00e9rez, M., Feria Arroyo, T.P., Venegas Barrera, C.S., Sosa-Guti\u00e9rrez, C., Torres, J., Brown, K.A., and Gordillo P\u00e9rez, G. (2023). Predicting the Impact of Climate Change on the Distribution of Rhipicephalus sanguineus in the Americas. Sustainability, 15.","DOI":"10.3390\/su15054557"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1756-3305-3-26","article-title":"Biology and ecology of the brown dog tick, Rhipicephalus sanguineus","volume":"3","year":"2010","journal-title":"Parasites Vectors"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetpar.2011.04.015","article-title":"Perspectives on canine and feline hepatozoonosis","volume":"181","author":"Baneth","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"82","DOI":"10.17533\/udea.rccp.v35n2a01","article-title":"Prevalence of Ehrlichia canis and Hepatozoon canis in sheltered dogs in southern Aburr\u00e1 Valley, Colombia","volume":"35","author":"Monsalve","year":"2022","journal-title":"Rev. Colomb. Cienc. Pecu."},{"key":"ref_51","first-page":"101132","article-title":"Molecular screening of selected vector-borne pathogens circulating in owned dogs in the Caribbean Archipelago of Guadeloupe (France)","volume":"56","author":"Imbert","year":"2024","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_52","first-page":"101027","article-title":"Molecular Survey of Piroplasmida, Hepatozoon spp. and Anaplasmataceae in anemic and thrombocytopenic dogs from Uruguay","volume":"51","author":"Parodi","year":"2024","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"388","DOI":"10.5216\/cab.v13i3.18439","article-title":"Detec\u00e7\u00e3o molecular de Babesia canis vogeli em c\u00e3es e em Rhipicephalus sanguineus na Mesorregi\u00e3o do Oeste Maranhense, Nordeste brasileiro","volume":"13","author":"Silva","year":"2012","journal-title":"Ci\u00eanc. Anim. Bras."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.5433\/1679-0359.2020v41n5supl1p2143","article-title":"Molecular detection of Babesia vogeli, Ehrlichia canis and Anaplasma platys in a hospital population of dogs clinically diagnosed with hemoparasitosis","volume":"41","author":"Paschoal","year":"2020","journal-title":"Semin. Ci\u00eanc. Agr\u00e1r."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e012420","DOI":"10.1590\/s1984-29612020057","article-title":"Molecular evaluation of piroplasms and hematological changes in canine blood stored in a clinical laboratory in Niter\u00f3i, Rio de Janeiro","volume":"29","author":"Santos","year":"2020","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_56","first-page":"90","article-title":"Hematological and epidemiological characterization of Hepatozoon canis infection in dogs from Buenos Aires, Argentina","volume":"8","author":"Vezzani","year":"2017","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_57","first-page":"1002","article-title":"Dogs naturally infected with Hepatozoon spp. in the Sert\u00e3o Paraibano Mesoregion, Brazilian Semiarid","volume":"52","author":"Bernardino","year":"2024","journal-title":"Acta Sci. Vet."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1590\/S0100-736X2015000500012","article-title":"Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernambuco, Brazil","volume":"35","author":"Araujo","year":"2015","journal-title":"Pesq. Vet. Bras."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"163","DOI":"10.5433\/1679-0359.2016v37n1p163","article-title":"Parasitological and molecular detection of Babesia canis vogeli in dogs of Recife, Pernambuco and evaluation of risk factors associated","volume":"37","author":"Silva","year":"2016","journal-title":"Semin. Ci\u00eanc. Agr\u00e1r."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e20190389","DOI":"10.1590\/0103-8478cr20190389","article-title":"Molecular prevalence and factors associated with Babesia vogeli infection in dogs in the Cerrado Mato-Grossense region of Brazil","volume":"50","author":"Castro","year":"2020","journal-title":"Cienc. Rural"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Ramos, R.C.F., Barbosa, A.D.S., Aleixo, A.L.Q.D.C., Arruda, I.F., and Amendoeira, M.R.R. (2024). Comparison of immunological and molecular methods for laboratory diagnosis of ocular toxoplasmosis in blood, serum and tears in Brazil. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0298393"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.ttbdis.2014.03.011","article-title":"Arthropod-borne pathogens circulating in free-roaming domestic cats in a zoo environment in Brazil","volume":"5","author":"Henrique","year":"2014","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.ttbdis.2015.07.004","article-title":"Tick-Borne agents in domesticated and stray cats from the city of Campo Grande, State of Mato Grosso Do Sul, Midwestern Brazil","volume":"6","author":"Herrera","year":"2015","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Kelly, P.J., K\u00f6ster, L., Li, J., Zhang, J., Huang, K., Branford, G.C., Marchi, S., Vandenplas, M., and Wang, C. (2017). Survey of vector-borne agents in feral cats and first report of Babesia gibsoni in cats on St Kitts, West Indies. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1230-1"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"23","DOI":"10.4322\/rbpv.01802005","article-title":"Babesia spp. Infection in dogs from rural areas of S\u00e3o Paulo state, Brazil","volume":"18","author":"Lopes","year":"2009","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.5433\/1679-0359.2019v40n3p1357","article-title":"Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of midwestern Brazil","volume":"40","author":"Maia","year":"2019","journal-title":"Semin. Ci\u00eanc. Agr\u00e1r."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"35577","DOI":"10.34117\/bjdv6n6-191","article-title":"Nolecular detection of Leishmania infantum, Babesia vogeli, and Rangelia vitalii in dogs from the Embu-Gua\u00e7\u00fa municipality around the Guarapiranga reservoir, S\u00e3o Paulo","volume":"6","author":"Castelli","year":"2020","journal-title":"Braz. J. Dev."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"e200761","DOI":"10.11606\/issn.1678-4456.bjvras.2023.200761","article-title":"C\u00e3es naturalmente infectados por rangelia vitalii, Babesia canis vogeli, e Ehrlichia canis em S\u00e3o Paulo, Brasil","volume":"60","author":"Batista","year":"2023","journal-title":"Braz. J. Vet. Res. Anim. Sci."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"102181","DOI":"10.1016\/j.ttbdis.2023.102181","article-title":"Canine Piroplasmids: Molecular detection and laboratory characterization in dogs from Brasilia, Brazil, with the first molecular evidence of dog exposure to a novel opossum-associated Babesia sp.","volume":"14","author":"Yang","year":"2023","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.vetpar.2006.04.036","article-title":"Babesia gibsoni genotype Asia in dogs from Brazil","volume":"141","author":"Trapp","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1590\/s1984-29612017040","article-title":"Clinical and hematological evaluation of Rangelia vitalii naturally infected dogs in Southeastern Brazil","volume":"26","author":"Lemos","year":"2017","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.vetpar.2009.05.002","article-title":"Hepatozoon canis infecting dogs in the state of Esp\u00edrito Santo, Southeastern Brazil","volume":"163","author":"Spolidorio","year":"2009","journal-title":"Vet. Parasitol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1590\/S1984-29612015019","article-title":"Molecular identification of Hepatozoon canis in dogs from Campo Grande, Mato grosso do Sul, Brazil","volume":"24","author":"Ramos","year":"2015","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.vetpar.2017.02.023","article-title":"Molecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route","volume":"237","author":"Fernandes","year":"2017","journal-title":"Vet. Parasitol"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1590\/s1984-296120180069","article-title":"Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern Brazil","volume":"27","author":"Mongruel","year":"2018","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1756-3305-4-55","article-title":"Diagnosis of Hepatozoon canis in young dogs by cytology and PCR","volume":"4","author":"Otranto","year":"2011","journal-title":"Parasites Vectors"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1645\/0022-3395(2003)089[0379:MCOHC]2.0.CO;2","article-title":"Monozoic cysts of Hepatozoon canis","volume":"89","author":"Baneth","year":"2003","journal-title":"J. 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