{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T08:46:01Z","timestamp":1775637961971,"version":"3.50.1"},"reference-count":24,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fundamental Fund of Khon Kaen University from National Science, Research and Innovation Fund 2025, Thailand"},{"name":"Office of the Ministry of Higher Education, Science, Research, and Innovation under the Reinventing University 2026 Visiting Professor Program"},{"name":"Research Program on Emerging and Re-Emerging Infectious Diseases from the Japan Agency for Medical Research and Development","award":["23fk0108681h0901"],"award-info":[{"award-number":["23fk0108681h0901"]}]},{"name":"JSPS KAKENHI","award":["JP20K08816"],"award-info":[{"award-number":["JP20K08816"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: Human cysticercosis, caused by the larval stage (cysticerci) of the pork tapeworm Taenia solium, is an important zoonotic disease. The disease is prevalent in developing countries where porcine cysticercosis is common and undercooked pork is habitually consumed. Objective: This study aimed to develop an immunochromatography-based test kit for the rapid diagnosis of human cysticercosis using low-molecular-weight antigens purified from cyst fluid of the T. solium Asian genotype to detect specific IgG antibodies in whole blood. The kit was designated as \u201cthe cysticercosis whole-blood test kit (iCys WB kit).\u201d Methods: It was evaluated under laboratory conditions using 164 whole-blood samples, of which 21 were from confirmed cysticercosis cases. The results of the iCys WB kit, which detects anti-T. solium (cysticercus) antibodies in simulated whole blood samples, were compared with results from corresponding human serum samples. Results: When using both sample types, iCys WB kit demonstrated an accuracy of 98.8%, a sensitivity of 91.7%, a specificity of 100%, a positive likelihood ratio of 0, a negative likelihood ratio of 0.083, and an ROC area of 0.96. The agreement between results obtained from simulated whole-blood and serum samples showed perfect concordance. Conclusions: The iCys WB kit is a valuable easy-to-handle diagnostic tool and may be applicable for supporting clinical diagnosis at the point of care.<\/jats:p>","DOI":"10.3390\/pathogens15040399","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T07:25:12Z","timestamp":1775633112000},"page":"399","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Whole-Blood Point-of-Care Test for Highly Specific Serodiagnosis of Human Cysticercosis"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0462-6209","authenticated-orcid":false,"given":"Lakkhana","family":"Sadaow","sequence":"first","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patcharaporn","family":"Boonroumkaew","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rutchanee","family":"Rodpai","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0451-0756","authenticated-orcid":false,"given":"Oranuch","family":"Sanpool","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2604-0281","authenticated-orcid":false,"given":"Tongjit","family":"Thanchomnang","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9204-0602","authenticated-orcid":false,"given":"Marcello Otake","family":"Sato","sequence":"additional","affiliation":[{"name":"Division of Global Environment Parasitology, Faculty of Medical Technology, Niigata University of Pharmacy and Medical and Life Sciences, Niigata City 956-8603, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2138-8783","authenticated-orcid":false,"given":"Pewpan M.","family":"Intapan","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Parasitology, National Institute of Infectious Diseases, Japan Institute for Health Security, Shinjuku-ku 162-8640, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhito","family":"Sako","sequence":"additional","affiliation":[{"name":"Division of Parasitology, Department of Infectious Diseases, Asahikawa Medical University, Asahikawa 078-8510, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toni","family":"Wandra","sequence":"additional","affiliation":[{"name":"Directorate of Postgraduate, Sari Mutiara Indonesia University, Kota Medan 20123, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kadek","family":"Swastika","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Medicine, Udayana University, Kota Denpasar 80232, Indonesia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6726-3759","authenticated-orcid":false,"given":"Wanchai","family":"Maleewong","sequence":"additional","affiliation":[{"name":"Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen 40002, Thailand"},{"name":"Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,7]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2025, November 14). 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