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This study evaluated the antifungal efficacy and safety of clinically relevant antiseptics against 60 C. parapsilosis clinical isolates using a surface carrier test designed to simulate contamination and disinfection events on hospital surfaces. Antifungal activity was assessed by logarithmic reduction (log10) assays on surface carriers and by minimum inhibitory concentration (MIC) testing. Potential synergistic interactions between antiseptics and selected phytochemicals were investigated using checkerboard assays, and toxicity was evaluated in vivo using Caenorhabditis elegans. Surface carrier assays showed that 70% ethanol and 0.5% alcoholic chlorhexidine (CHG) achieved the highest fungicidal activity, with reductions of up to 5 log10 after 1 min exposure at 25 \u00b0C. Polyhexamethylene guanidine hydrochloride (PHMGH) displayed consistently low MIC values (0.4\u20130.9 ppm) and intermediate surface activity. CHG combined with eugenol or menthol produced strong synergistic interactions, reducing CHG MICs from up to 6250 ppm to as low as 20 ppm (&gt;300-fold). Toxicity assays revealed a narrow safety margin for CHG, whereas PHMGH showed a more gradual concentration-dependent toxicity profile. These findings highlight clinically relevant differences in antiseptic performance and identify combination strategies that may reduce CHG exposure while maintaining antifungal efficacy.<\/jats:p>","DOI":"10.3390\/pathogens15040410","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T09:08:12Z","timestamp":1775812092000},"page":"410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Surface Carrier Testing of Hospital Antiseptics Against Candida parapsilosis from Healthcare Workers\u2019 Hands"],"prefix":"10.3390","volume":"15","author":[{"given":"Jenyffie Ara\u00fajo","family":"Beliz\u00e1rio","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, University of Franca, Franca 14404-600, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Eduarda Brites","family":"Jardine","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, University of Franca, Franca 14404-600, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle Lameado","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, University of Franca, Franca 14404-600, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3380-9603","authenticated-orcid":false,"given":"Murilo Molina","family":"Stefani","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, University of Franca, Franca 14404-600, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8499-9090","authenticated-orcid":false,"given":"Ralciane de Paula","family":"Menezes","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise von Dolinger de Brito","family":"R\u00f6der","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3010-5754","authenticated-orcid":false,"given":"Reginaldo dos Santos","family":"Pedroso","sequence":"additional","affiliation":[{"name":"Technical School of Health, Federal University of Uberl\u00e2ndia, Uberl\u00e2ndia 38408-100, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Ricardo","family":"Ambr\u00f3sio","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, University of Franca, Franca 14404-600, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5469-3602","authenticated-orcid":false,"given":"Gil","family":"Benard","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Mycology (LIM-53), Institute of Tropical Medicine, Hospital das Cl\u00ednicas, Department of Dermatology, Faculty of Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-220, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6004-5951","authenticated-orcid":false,"given":"Regina Helena","family":"Pires","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, University of Franca, Franca 14404-600, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Thomaz, D.Y., de Almeida, J.N., Lima, G.M.E., Nunes, M.O., Camargo, C.H., Grenfell, R.C., Benard, G., and Del Negro, G.M. 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