{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:57:38Z","timestamp":1791601058690,"version":"4.3.4"},"reference-count":66,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T00:00:00Z","timestamp":1776038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Silesia","award":["BNW-1-017\/N\/5\/I"],"award-info":[{"award-number":["BNW-1-017\/N\/5\/I"]}]},{"award":["BNW-1-017\/N\/5\/I"],"award-info":[{"award-number":["BNW-1-017\/N\/5\/I"]}],"id":[{"id":"https:\/\/ror.org\/005k7hp45","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Slovak Research and Development Agency","doi-asserted-by":"crossref","award":["APVV-SK-PL-25-0018"],"award-info":[{"award-number":["APVV-SK-PL-25-0018"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"crossref"}]},{"award":["APVV-SK-PL-25-0018"],"award-info":[{"award-number":["APVV-SK-PL-25-0018"]}],"id":[{"id":"https:\/\/ror.org\/037nx0e70","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Slovak Scientific Grant Agency VEGA","award":["2\/0033\/25"],"award-info":[{"award-number":["2\/0033\/25"]}]},{"name":"Slovak Scientific Grant Agency VEGA","award":["2\/0051\/24"],"award-info":[{"award-number":["2\/0051\/24"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Haematopota pluvialis is a widely distributed hematophagic insect occurring across Eurasia. This horse fly may be a highly efficient mechanical vector of pathogens, including viruses, bacteria, and protozoa. Furthermore, its painful bites can cause local skin lesions and systemic symptoms. The aim of this study was to determine human exposure to H. pluvialis attacks in various types of open space habitats in Eastern Poland and to perform molecular screening of these tabanids for the presence of hematopathogens: Bartonella spp. and Anaplasma phagocytophilum. Specimens of H. pluvialis were collected at three distinct sites in Eastern Poland. The presence of Bartonella spp. and A. phagocytophilum was investigated using PCR-based methods. In total, 141 H. pluvialis females were analyzed. The molecular analysis of the rpoB gene fragment yielded one new haplotype of Bartonella sp. in 0.7% (1) of all studied samples, which may hypothetically exhibit zoonotic potential. Anaplasma phagocytophilum was not detected in the studied material. Moreover, a high level of human and animals exposure to horse fly bites was noted in the studied areas of Eastern Poland. The present results highlight the need for further targeted research on H. pluvialis to quantify pathogen prevalence, transmission efficiencies, and conditions facilitating pathogen transmission in natural settings.<\/jats:p>","DOI":"10.3390\/pathogens15040417","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T10:19:14Z","timestamp":1776075554000},"page":"417","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["New Haplotype of Bartonella sp. in Haematopota pluvialis (Linnaeus, 1758)"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4527-9012","authenticated-orcid":false,"given":"Katarzyna","family":"Bartosik","sequence":"first","affiliation":[{"name":"Department of Biology and Parasitology, Chair of Pharmacology and Biology, Faculty of Health Sciences, Medical University of Lublin, Radziwi\u0142\u0142owska 11 St., 20-080 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1808-9037","authenticated-orcid":false,"given":"Magdalena","family":"\u015awis\u0142ocka-Cutter","sequence":"additional","affiliation":[{"name":"Department of Zoology and Genetics, Faculty of Biology, University of Bialystok, Ciolkowskiego 1J, 15-245 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Werszko","sequence":"additional","affiliation":[{"name":"Department of General Biology and Parasitology, Medical University of Warsaw, Cha\u0142ubi\u0144skiego 5, 02-004 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Aftyka","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiological and Intensive Care Nursing, Medical University of Lublin, ul. Chod\u017aki 7, 20-093 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1871-3362","authenticated-orcid":false,"given":"Klaudia M\u00e1ria","family":"\u0160virlochov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"},{"name":"Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Ko\u0161ice, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0882-7806","authenticated-orcid":false,"given":"Dana","family":"Zubrikov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6538-3044","authenticated-orcid":false,"given":"Bronislava","family":"V\u00edchov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2742-3989","authenticated-orcid":false,"given":"Magdalena","family":"Raszewska-Famielec","sequence":"additional","affiliation":[{"name":"Faculty of Physical Education and Health, University of Physical Education, 21-500 Biala Podlaska, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8832-7726","authenticated-orcid":false,"given":"Marek","family":"Asman","sequence":"additional","affiliation":[{"name":"Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Jordana 19 St., 41-808 Zabrze, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,13]]},"reference":[{"key":"ref_1","unstructured":"(2025, November 20). 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