{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:42:23Z","timestamp":1785436943697,"version":"3.56.0"},"reference-count":27,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T00:00:00Z","timestamp":1776211200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005668","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Distrito Federal","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Sanitization of hatching eggs is part of established poultry management practices, and its effectiveness is essential for productive success. This study aimed to investigate the relationships between microbiological and incubation performance variables obtained from a controlled experimental dataset of hatching eggs subjected to sanitization with essential oils or not under commercial conditions, and to determine the efficacy of these sanitizers, using a multivariate approach. Data were analyzed using principal component, canonical, cluster, and discriminant analysis. The results suggested that bacterial contamination of the eggshell may promote internal contamination, leading to embryonic mortality. Essential oil-based treatments are associated with lower microbial indicators and improved hatchability, while formaldehyde showed an opposite trend despite its antibacterial efficacy. Multivariate analyses clarified the interrelationships between microbiological and incubation performance variables, allowing the identification of response patterns that evidenced the functional efficiency of essential oil\u2013based treatments for hatching egg sanitization under commercial conditions.<\/jats:p>","DOI":"10.3390\/pathogens15040426","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T08:11:18Z","timestamp":1776240678000},"page":"426","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multivariate Assessment of Microbiological and Incubation Data from an Experimental Trial Evaluating Essential Oil\u2013Based Sanitizers and Formaldehyde on Hatching Eggs"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1249-0230","authenticated-orcid":false,"given":"Vin\u00edcius Machado","family":"dos Santos","sequence":"first","affiliation":[{"name":"Laboratory of Poultry Science, Federal Institute of Bras\u00edlia\u2014Campus Planaltina, Bras\u00edlia 73380-900, Brazil"},{"name":"Faculty of Agronomy and Veterinary Medicine, University of Bras\u00edlia, Bras\u00edlia 70910-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2948-6652","authenticated-orcid":false,"given":"Gabriel da Silva","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Faculty of Agronomy and Veterinary Medicine, University of Bras\u00edlia, Bras\u00edlia 70910-900, Brazil"},{"name":"Faculty of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concepta","family":"McManus","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-270, Brazil"},{"name":"Center for Nuclear Energy in Agriculture (CENA), University of S\u00e3o Paulo, S\u00e3o Paulo 13416-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Oliveira, G.d.S., McManus, C., and dos Santos, V.M. (2025). Multivariate Analysis of Microbiological and Incubation Parameters in Hatching Eggs Sanitized With or Without Essential Oils. Vet. Sci., 12.","DOI":"10.3390\/vetsci12070600"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2466","DOI":"10.3382\/ps\/pez022","article-title":"An Evaluation of Alternative Methods for Sanitizing Hatching Eggs","volume":"98","author":"Melo","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2852","DOI":"10.1080\/09603123.2023.2276361","article-title":"The effects of using UV light instead of formaldehyde in disinfection of hatching eggs on shell microbial load, embryo development, hatchability, and chick characteristics","volume":"34","author":"Coulibaly","year":"2024","journal-title":"Int. J. Environ. Health Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2478","DOI":"10.1016\/j.psj.2019.12.039","article-title":"Use of Reactive Oxygen Species (Ozone, Hydrogen Peroxide) for Disinfection of Hatching Eggs","volume":"99","author":"Wlazlo","year":"2020","journal-title":"Poult. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.21608\/epsj.2018.22699","article-title":"Comparative Study of the Effect of Some Disinfectants on Embryonic Mortality, Hatchability, and Some Blood Components","volume":"38","author":"Badran","year":"2018","journal-title":"Egypt. Poult. Sci. J."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2372","DOI":"10.3382\/ps\/pey102","article-title":"Evaluation of Propolis Extract as a Disinfectant of Japanese Quail (Coturnix coturnix Japonica) Hatching Eggs","volume":"97","author":"Batkowska","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"291","DOI":"10.21608\/epsj.2019.29843","article-title":"Effect of Spraying Hatching Eggs by Different Levels of Vinegar on Embryological Development, Hatchability and Physiological Performance of Dandarwi Chicks","volume":"39","author":"Fouad","year":"2019","journal-title":"Egypt. Poult. Sci. J."},{"key":"ref_8","first-page":"45","article-title":"Comparison of Virkon S and Formaldehyde on Hatchability and Survival Rate of Chicks in Disinfection of Fertile Eggs","volume":"167","author":"Shahzamani","year":"2016","journal-title":"Rev. Med. Vet."},{"key":"ref_9","first-page":"193","article-title":"The Effect of Various Disinfectants on Hatching Results in Chickens","volume":"54","author":"Hanus","year":"2021","journal-title":"Sci. Pap. Anim. Sci. Biotechnol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"El-Soufi, A., Al Khatib, A., Khazaal, S., El Darra, N., and Raafat, K. (2025). Evaluation of essential oils as natural antibacterial agents for eggshell sanitization and quality preservation. Processes, 13.","DOI":"10.3390\/pr13010224"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1080\/01919512.2021.1967722","article-title":"Antifungal Action of Ozone on Chicken Eggshell Cuticles: A Preliminary Study","volume":"44","author":"Soares","year":"2022","journal-title":"Ozone Sci. Eng."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Oliveira, G.D.S., McManus, C., Santos, P.H.G.D.S., de Sousa, D.E.R., Jivago, J.L.D.P.R., de Castro, M.B., and dos Santos, V.M. (2024). Hatching egg sanitizers based on essential oils: Microbiological parameters, hatchability, and poultry health. Antibiotics, 13.","DOI":"10.3390\/antibiotics13111066"},{"key":"ref_13","first-page":"1","article-title":"Rosemary Essential Oil as a Natural Disinfectant in Japanese Quail Eggs (Coturnix coturnix Japonica): Effect on the Bacterial Load and Hatchability Characteristics","volume":"13","author":"Touazi","year":"2025","journal-title":"Int. J. Vet. Sci. Med."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.foodcont.2017.08.015","article-title":"Antimicrobial and antioxidant efficiency of nanoemulsion-based edible coating containing ginger (Zingiber officinale) essential oil and its effect on safety and quality attributes of chicken breast fillets","volume":"84","author":"Noori","year":"2018","journal-title":"Food Control"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Oliveira, G.D.S., McManus, C., Pires, P.G.D.S., and dos Santos, V.M. (2022). Combination of Cassava Starch Biopolymer and Essential Oils for Coating Table Eggs. Front. Sustain. Food Syst., 6.","DOI":"10.3389\/fsufs.2022.957229"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3939","DOI":"10.1002\/fsn3.3380","article-title":"Plectranthus amboinicus and rosemary (Rosmarinus officinalis L.) essential oils effects on performance, antioxidant activity, intestinal health, immune response, and plasma biochemistry in broiler chickens","volume":"11","author":"Hosseinzadeh","year":"2023","journal-title":"Food Sci. Nutr."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"dos Santos, V.M., Oliveira, G.D.S., Santos, P.H.G.D.S., Cerqueira, L.D.A., Jivago, J.L.D.P.R., Paix\u00e3o, S.S.R.M., de Castro, M.B., and McManus, C. (2025). Measuring Contamination Levels and Incubation Results of Hatching Eggs Sanitized with Essential Oils. Antibiotics, 14.","DOI":"10.3390\/antibiotics14111076"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1007\/s11250-025-04556-8","article-title":"Egg sanitation with ginger and garlic solutions affects embryonic development, hatchability, blood parameters and post-hatch performance of Japanese quail","volume":"57","year":"2025","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1399\/eps.2017.211","article-title":"Effect of Using Colloidal Silver in the Disinfection of Hatching Eggs on Some Microbial, Hatchability and Performance Traits in Japanese Quail (Coturnix Cot. Japonica)","volume":"81","author":"Batkowska","year":"2017","journal-title":"Eur. Poult. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.21608\/epsj.2017.5655","article-title":"Effect of Spraying Hatching Eggs of Japanese Quails by Live Yeast on Physiological Changes in the Embryonic Development, Hatchability and Total Bacterial Count","volume":"37","author":"Fouad","year":"2017","journal-title":"Egypt. Poult. Sci. J."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1080\/09712119.2022.2138894","article-title":"Essential Oil Sanitizers to Sanitize Hatching Eggs","volume":"50","author":"Bekhet","year":"2022","journal-title":"J. Appl. Anim. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jobaz.2014.12.005","article-title":"Comparing the Effectiveness of Egg Disinfectants against Bacteria and Mitotic Indices of Developing Chick Embryos","volume":"70","author":"Zeweil","year":"2015","journal-title":"J. Basic Appl. Zool."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Iraqi, E.E., El-Sahn, A.A., El-Barbary, A.M., Ahmed, M.M., and Elkomy, A.E. (2025). Antimicrobial activity of tea tree and lavender essential oils and their effects on hatching performance and eggshell bacterial count of Japanese quail eggs. BMC Vet. Res., 21.","DOI":"10.1186\/s12917-025-04576-4"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"623","DOI":"10.4315\/0362-028X-70.3.623","article-title":"Eggshell penetration of various types of hens\u2019 eggs by Salmonella enterica serovar Enteritidis","volume":"70","author":"Messens","year":"2007","journal-title":"J. Food Prot."},{"key":"ref_25","first-page":"828","article-title":"Demystifying the power of essential oils: A review of their antibacterial properties and potential as natural food preservatives","volume":"24","author":"Falleh","year":"2025","journal-title":"EXCLI J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1128\/CMR.12.1.147","article-title":"Antiseptics and Disinfectants: Activity, Action, and Resistance","volume":"12","author":"McDonnell","year":"1999","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1016\/j.molcel.2025.04.018","article-title":"Emerging Mechanisms Underlying Formaldehyde Toxicity and Response","volume":"85","author":"Pontel","year":"2025","journal-title":"Mol. 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