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The ability to survive long periods without food promotes the persistence of this vector in the environment and contributes to its evolutionary success. This study employed one- and two-dimensional electrophoretic techniques combined with Western blot to evaluate the abundance of \u03b1-tubulin, \u03b2-actin, and the heat shock protein HSP70. These proteins were more abundant in the anterior midgut tissue of unfed insects than in that of fed insects. As these responses were similar in females and males, the observed adaptations primarily depend on feeding status and intestinal region. These findings provide further insight into the intestinal physiology of T. pallidipennis, a vector of the flagellate parasite Trypanosoma cruzi, the causative agent of Chagas disease.<\/jats:p>","DOI":"10.3390\/pathogens15050482","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T10:47:28Z","timestamp":1777546048000},"page":"482","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Food Deprivation in Triatoma pallidipennis Increases the Expression of \u03b1-Tubulin, \u03b2-Actin, and a Heat Shock Protein in the Anterior Midgut"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0046-6731","authenticated-orcid":false,"given":"Olivia Alicia","family":"Reynoso-Ducoing","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5340-7867","authenticated-orcid":false,"given":"Elsa Gabriela","family":"D\u00edaz-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8829-9777","authenticated-orcid":false,"given":"Elia","family":"Torres-Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9414-1968","authenticated-orcid":false,"given":"Mauro Omar","family":"Vences-Blanco","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6883-8000","authenticated-orcid":false,"given":"Berenice","family":"Gonz\u00e1lez-Rete","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Guevara-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8639-2612","authenticated-orcid":false,"given":"Margarita","family":"Cabrera-Bravo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8490-5293","authenticated-orcid":false,"given":"Paz Mar\u00eda Silvia","family":"Salazar-Schettino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, M\u00e9xico City 04510, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2026, February 11). Chagas Disease (American Trypanosomiasis). Available online: https:\/\/www.who.int\/es\/news-room\/fact-sheets\/detail\/chagas-disease-(american-trypanosomiasis)."},{"key":"ref_2","unstructured":"Pan American Health Organization (2026, February 11). Chagas Disease. Available online: https:\/\/www.paho.org\/en\/topics\/chagas-disease."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"57","DOI":"10.36610\/j.jsars.2010.100100057x","article-title":"Revisi\u00f3n de 13 especies de la familia Triatominae (Hemiptera: Reduviidae) vectores de la enfermedad de Chagas, en M\u00e9xico","volume":"1","year":"2010","journal-title":"J. Selva Andin. Res. Soc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1111\/j.1948-7134.2012.00255.x","article-title":"Bionomics of populations of Meccus pallidipennis (St\u00e5l, 1872) (Hemiptera: Reduviidae) from Mexico","volume":"37","year":"2012","journal-title":"J. Vector Ecol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/nature19949","article-title":"Mass-spectrometric exploration of proteome structure and function","volume":"537","author":"Aebersold","year":"2016","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ouali, R., Valentim de Brito, K.C., Salmon, D., and Bousbata, S. (2020). High-throughput identification of the Rhodnius prolixus midgut proteome unravels a sophisticated hematophagic machinery. Proteomes, 8.","DOI":"10.3390\/proteomes8030016"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/S0169-4758(00)01724-5","article-title":"The development of Trypanosoma cruzi in Triatominae","volume":"16","author":"Kollien","year":"2000","journal-title":"Parasitol. Today"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Ouali, R., Vieira, L.R., Salmon, D., and Bousbata, S. (2021). Early post-prandial regulation of protein expression in the midgut of Rhodnius prolixus. Microorganisms, 9.","DOI":"10.3390\/microorganisms9040804"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ribeiro, J.M.C., Genta, F.A., Sorgine, M.H.F., Logullo, R., Mesquita, R.D., Paiva-Silva, G.O., Majerowicz, D., Medeiros, M., Koerich, L., and Terra, W.R. (2014). An insight into the transcriptome of the digestive tract of the bloodsucking bug, Rhodnius prolixus. PLoS Neglected Trop. Dis., 8.","DOI":"10.1371\/journal.pntd.0002594"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"a027896","DOI":"10.1101\/cshperspect.a027896","article-title":"Microtubule motors in establishment of epithelial cell polarity","volume":"10","author":"Kreitzer","year":"2018","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Herrmann, H., and Strelkov, S.V. (2011). History and phylogeny of intermediate filaments: Now in insects. BMC Biol., 9.","DOI":"10.1186\/1741-7007-9-16"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Terra, W.R., Ferreira, C., and Silva, C.P. (2023). Molecular Physiology and Evolution of Insect Digestive Systems, Springer. Entomology in Focus.","DOI":"10.1007\/978-3-031-39233-7"},{"key":"ref_13","unstructured":"Gilbert, L.I. (2012). Biochemistry and molecular biology of digestion. Insect Molecular Biology and Biochemistry, Academic Press."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.crvi.2010.02.001","article-title":"Digestive cells in the midgut of Triatoma vitticeps (St\u00e5l, 1859) in different starvation periods","volume":"333","author":"Rocha","year":"2010","journal-title":"C. R. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.jinsphys.2012.10.015","article-title":"Midgut proteins released by microapocrine secretion in Spodoptera frugiperda","volume":"59","author":"Silva","year":"2013","journal-title":"J. Insect Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1112","DOI":"10.1016\/j.jinsphys.2007.06.007","article-title":"Identification of midgut microvillar proteins from Tenebrio molitor F and Spodoptera frugiperda by cDNA library screening with antibodies","volume":"53","author":"Ferreira","year":"2007","journal-title":"J. Insect Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.ibmb.2016.07.011","article-title":"Functional evaluation of heat shock proteins 70 (HSP70\/HSC70) on Rhodnius prolixus physiological responses associated with feeding and starvation","volume":"77","author":"Paim","year":"2016","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1186\/s13071-016-1398-4","article-title":"Impact of Trypanosoma cruzi on antimicrobial peptide gene expression and activity in the fat body and midgut of Rhodnius prolixus","volume":"9","author":"Vieira","year":"2016","journal-title":"Parasit. Vectors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1083\/jcb.67.3.725","article-title":"Organization of an actin filament\u2013membrane complex","volume":"67","author":"Mooseker","year":"1975","journal-title":"J. Cell Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1534\/genetics.118.300224","article-title":"Anatomy and physiology of the digestive tract of Drosophila melanogaster","volume":"210","author":"Jasper","year":"2018","journal-title":"Genetics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"101383","DOI":"10.1016\/j.asd.2024.101383","article-title":"Ultrastructure of the larval rectum of Panorpa liui","volume":"82","author":"Ma","year":"2024","journal-title":"Arthropod Struct. Dev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/S0022-1910(00)00170-0","article-title":"Ionic composition of the rectal contents and excreta of the reduviid bug Triatoma infestans","volume":"47","author":"Kollien","year":"2001","journal-title":"J. Insect Physiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1002\/cm.20547","article-title":"Regulation of epithelial permeability by the actin cytoskeleton","volume":"68","author":"Rodgers","year":"2011","journal-title":"Cytoskeleton"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Gumiel, M., de Mattos, D.P., Vieira, C.S., Moraes, C.S., Moreira, C.J.C., Gonzalez, M.S., Teixeira-Ferreira, A., Waghabi, M., Azambuja, P., and Carels, N. (2020). Proteome of the triatomine digestive tract: From catalytic to immune pathways, focusing on annexin expression. Front. Mol. Biosci., 7.","DOI":"10.3389\/fmolb.2020.589435"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Orchard, I., Lee, D.H., da Silva, R., and Lange, A.B. (2011). The proctolin gene and biological effects of proctolin in the blood-feeding bug, Rhodnius prolixus. Front. Endocrinol., 2.","DOI":"10.3389\/fendo.2011.00059"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"8026","DOI":"10.1073\/pnas.1105195108","article-title":"Drinking a hot blood meal elicits a protective heat shock response in mosquitoes","volume":"108","author":"Benoit","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Carvalho-Costa, T.M., Tiveron, R.D.R., Mendes, M.T., Barbosa, C.G., Nevoa, J.C., Roza, G.A., Silva, M.V., Figueiredo, H.C.P., Rodrigues, V., and Soares, S.D.C. (2021). Salivary and intestinal transcriptomes reveal differential gene expression in starving, fed and Trypanosoma cruzi-infected Rhodnius neglectus. Front. Cell. Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.773357"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.exppara.2006.11.012","article-title":"Trypanosoma cruzi: Attachment to perimicrovillar membrane glycoproteins of Rhodnius prolixus","volume":"116","author":"Alves","year":"2007","journal-title":"Exp. Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Gonz\u00e1lez-Rete, B., L\u00f3pez-Avi\u00f1a, J.A., Reynoso-Ducoing, O.A., Cabrera-Bravo, M., Bucio-Torres, M.I., Vences-Blanco, M.O., Torres-Guti\u00e9rrez, E., and Salazar-Schettino, P.M. (2025). Lectin recognition patterns in the gut of Meccus pallidipennis and their association with Trypanosoma cruzi metacyclogenesis. Microorganisms, 13.","DOI":"10.3390\/microorganisms13081823"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1111\/1744-7917.12579","article-title":"Glycosylation on proteins of the intestine and perimicrovillar membrane of Triatoma pallidipennis under different feeding conditions","volume":"26","author":"Zandberg","year":"2019","journal-title":"Insect Sci."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Torres-Guti\u00e9rrez, E., Candelas-Otero, F.N., Reynoso-Ducoing, O.A., Gonz\u00e1lez-Rete, B., Vences-Blanco, M.O., Cabrera-Bravo, M., Bucio-Torres, M.I., and Salazar-Schettino, P.M. (2025). Glycosylation patterns in Meccus pallidipennis gut: Implications for vector control strategies. Microorganisms, 13.","DOI":"10.3390\/microorganisms13010058"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0003-2697(81)90281-5","article-title":"Western blotting: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to nitrocellulose","volume":"112","author":"Burnette","year":"1981","journal-title":"Anal. Biochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"113608","DOI":"10.1016\/j.ab.2020.113608","article-title":"A systematic approach to quantitative Western blot analysis","volume":"593","author":"Harford","year":"2020","journal-title":"Anal. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"361590","DOI":"10.1155\/2014\/361590","article-title":"The design of a quantitative Western blot experiment","volume":"2014","author":"Taylor","year":"2014","journal-title":"BioMed Res. Int."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"429","DOI":"10.4103\/1947-2714.100998","article-title":"Western blot: Technique, theory, and troubleshooting","volume":"4","author":"Mahmood","year":"2012","journal-title":"N. Am. J. Med. Sci."},{"key":"ref_37","unstructured":"Zubair, M., and Launico, M.V. (2025). Western blot: Principles, procedures, and clinical applications. StatPearls, StatPearls Publishing."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"tjag031","DOI":"10.1093\/jme\/tjag031","article-title":"Effect of mixed infection with Trypanosoma cruzi and the fungus Metarhizium anisopliae on survival, parasitemia, and immune response in Triatoma pallidipennis (Hemiptera: Reduviidae)","volume":"63","author":"Toriello","year":"2026","journal-title":"J. Med. Entomol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/0022-2836(71)90461-X","article-title":"Paramyosin and the filaments of molluscan catch muscles","volume":"56","author":"Cohen","year":"1971","journal-title":"J. Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1007\/s00436-014-3846-4","article-title":"Expression of cytoskeletal proteins of Taenia crassiceps","volume":"113","author":"Landa","year":"2014","journal-title":"Parasitol. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.mimet.2015.08.008","article-title":"Improved Proteomic Analysis Following Trichloroacetic Acid Extraction of Bacillus anthracis Spore Proteins","volume":"118","author":"Wunschel","year":"2015","journal-title":"J. Microbiol. Methods"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1016\/j.ijpara.2005.01.013","article-title":"Analysing proteomic data","volume":"35","author":"Barrett","year":"2005","journal-title":"Int. J. Parasitol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cca.2007.07.003","article-title":"Actin, a reliable marker of internal control?","volume":"385","author":"Ruan","year":"2007","journal-title":"Clin. Chim. Acta"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.4161\/auto.21459","article-title":"The actin cytoskeleton participates in the early events of autophagosome formation upon starvation-induced autophagy","volume":"8","author":"Aguilera","year":"2012","journal-title":"Autophagy"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1016\/j.jinsphys.2006.09.007","article-title":"Upregulation of two actin genes and redistribution of actin during diapause and cold stress in the northern house mosquito, Culex pipiens","volume":"52","author":"Kim","year":"2006","journal-title":"J. Insect Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"32939","DOI":"10.1038\/srep32939","article-title":"Roles and regulation of autophagy and apoptosis in the remodelling of the lepidopteran midgut epithelium during metamorphosis","volume":"6","author":"Romanelli","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"116","DOI":"10.2174\/138161208783378716","article-title":"Autophagy in invertebrates: Insights into development, regeneration and body remodeling","volume":"14","author":"Tettamanti","year":"2008","journal-title":"Curr. Pharm. Des."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Li, R., Xiao, Y., Li, K., and Tian, L. (2022). Transcription and post-translational regulation of autophagy in insects. Front. Physiol., 13.","DOI":"10.3389\/fphys.2022.825202"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1146\/annurev-ento-011613-162107","article-title":"Insect heat shock proteins during stress and diapause","volume":"60","author":"King","year":"2015","journal-title":"Annu. Rev. Entomol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/5\/482\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:06:43Z","timestamp":1777547203000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/5\/482"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,30]]},"references-count":49,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["pathogens15050482"],"URL":"https:\/\/doi.org\/10.3390\/pathogens15050482","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,30]]}}}