{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:45:38Z","timestamp":1785422738617,"version":"3.56.0"},"reference-count":82,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T00:00:00Z","timestamp":1778112000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Flemish International Cooperation Agency"},{"DOI":"10.13039\/100018370","name":"Institute of Tropical Medicine","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100018370","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Instituto Nacional de Sa\u00fade"},{"id":[{"id":"https:\/\/ror.org\/03hq46410","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003130","name":"Research Foundation Flanders","doi-asserted-by":"crossref","award":["1S74321N"],"award-info":[{"award-number":["1S74321N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"award":["1S74321N"],"award-info":[{"award-number":["1S74321N"]}],"id":[{"id":"https:\/\/ror.org\/03qtxy027","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Malariology Unit"},{"name":"Belgium Directorate-General Development Cooperation and Humanitarian Aid","award":["FA5"],"award-info":[{"award-number":["FA5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-SP) remains the main strategy to prevent malaria in pregnancy. However, continued drug pressure may also contribute to the emergence of resistant parasites and impact the gametocyte carriage and subsequent infectiousness. Pregnant women are thought to be a potential reservoir for malaria transmission due to the increased carriage of gametocytes following long-lasting infections. We used molecular methods to examine 100 Plasmodium falciparum (P. falciparum) isolates collected from Mozambican women at delivery in 2014-15 to determine sulfadoxine-pyrimethamine (SP) resistance polymorphisms in P. falciparum dihydrofolate reductase (pfdhfr) and dihydropteroate synthetase (pfdhps) genes, as well as the presence of gametocytes by RT-qPCR. Overall, 54% and 7% of parasites harbored quintuple and sextuple pfdhfr\/pfdhps mutant haplotypes, respectively. Gametocytes were detected in 34% of isolates. Gametocyte carriage was significantly associated with quintuple mutant infections (AOR = 7.5, p = 0.001), which accounted for 80% of infections with detectable gametocytes. Results indicate the relevance of ongoing surveillance of SP resistance in Mozambique to guide future evaluation of alternative intermittent preventive treatment in pregnancy (IPTp) approaches as resistance levels evolve and anticipate potential implications for parasite transmission and maternal\u2013fetal health.<\/jats:p>","DOI":"10.3390\/pathogens15050504","type":"journal-article","created":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T14:05:46Z","timestamp":1778162746000},"page":"504","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence of pfdhfr-pfdhps Sextuple and Gametocyte-Associated Quintuple Sulfadoxine-Pyrimethamine Resistance Mutations in Plasmodium falciparum Isolates from Pregnant Women in Mozambique"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7390-097X","authenticated-orcid":false,"given":"Yasmina","family":"Drissi-El Boukili","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"},{"name":"Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4765-1076","authenticated-orcid":false,"given":"Eduard","family":"Rovira-Vallbona","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"Guetens","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Driss","family":"Chiheb","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna Helena","family":"Kattenberg","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Kestens","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"},{"name":"Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia Maria Mauricio","family":"Enosse","sequence":"additional","affiliation":[{"name":"Divis\u00e3o de Investiga\u00e7\u00e3o em Sa\u00fade e Bem-Estar, Instituto Nacional de Sa\u00fade, Maputo 264, Mozambique"},{"name":"Malaria Consortium, Maputo 118, Mozambique"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Rosanas-Urgell","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8747-5209","authenticated-orcid":false,"given":"Paulo","family":"Arnaldo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Institute of Tropical Medicine, 2000 Antwerp, Belgium"},{"name":"Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium"},{"name":"Divis\u00e3o de Investiga\u00e7\u00e3o em Sa\u00fade e Bem-Estar, Instituto Nacional de Sa\u00fade, Maputo 264, Mozambique"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,7]]},"reference":[{"key":"ref_1","unstructured":"WHO (2025, August 10). 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