{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:01:06Z","timestamp":1785416466946,"version":"3.56.0"},"reference-count":30,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Ministry of Science and Higher Education of the Republic of Kazakhstan","award":["AP26198215"],"award-info":[{"award-number":["AP26198215"]}]},{"award":["AP26198215"],"award-info":[{"award-number":["AP26198215"]}],"id":[{"id":"https:\/\/ror.org\/05tyne317","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Helminth infections remain a major constraint to livestock productivity, particularly in regions where domestic animals and wildlife share grazing habitats. This study investigated the molecular diversity and transmission dynamics of helminth communities in sheep (Ovis aries) and saiga antelope (Saiga tatarica) in West Kazakhstan. A total of 35 animals (20 sheep and 15 saiga) were examined, and helminths were identified using polymerase chain reaction targeting the ITS1 region of ribosomal DNA for nematodes and the mitochondrial cox1 gene for cestodes. Of the 20 analyzed samples, 80% were successfully identified at the molecular level. Detected species included Haemonchus contortus, Trichuris ovis, Chabertia ovina, Moniezia expansa, and Avitellina centripunctata. Phylogenetic analysis revealed that Chabertia ovina isolates from both hosts clustered within a single monophyletic clade, indicating high genetic similarity and supporting potential cross-species transmission. Mitochondrial markers provided higher resolution for cestode differentiation, whereas ITS1 was effective for nematode identification. The predominance of Chabertia ovina in saiga suggests ecological adaptation and efficient transmission within wild populations. These findings highlight the epidemiological significance of shared grazing ecosystems and underscore the need for integrated parasite control strategies that consider both livestock and wildlife reservoirs.<\/jats:p>","DOI":"10.3390\/pathogens15050550","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T09:12:41Z","timestamp":1779268361000},"page":"550","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Epidemiology of Helminths at the Wildlife\u2013Livestock Interface in Kazakhstan: Evidence from Sheep and Saiga"],"prefix":"10.3390","volume":"15","author":[{"given":"Nurzhan","family":"Abekeshev","sequence":"first","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhangeldi","family":"Ussenov","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rinat","family":"Abdrakhmanov","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zukhra","family":"Aitpayeva","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Svotina","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhadyra","family":"Valiyeva","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Askhat","family":"Zhumabayev","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albina","family":"Darmenova","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilana","family":"Abirova","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhadyra","family":"Ryskaliyeva","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azamat","family":"Aitkaliyev","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine and Agrotechnology, Zhangir Khan West Kazakhstan Agrarian-Technical University, Oral 090000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aigul","family":"Kaliyeva","sequence":"additional","affiliation":[{"name":"Department of Biology, K. Zhubanov Aktobe Regional University, Aktobe 030000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anargul","family":"Berkaliyeva","sequence":"additional","affiliation":[{"name":"Department of Biology, K. Zhubanov Aktobe Regional University, Aktobe 030000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakhima","family":"Bissalyyeva","sequence":"additional","affiliation":[{"name":"Department of Biology, K. Zhubanov Aktobe Regional University, Aktobe 030000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Assylbek","family":"Zhanabayev","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulmira","family":"Abulgazimova","sequence":"additional","affiliation":[{"name":"Institute of Animal Science and Veterinary Medicine, Saken Seifullin Kazakh Agrotechnical University, Astana 010000, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1186\/s13071-022-05449-7","article-title":"Parasitic strongyle nemabiome communities in wild ruminants in Sweden","volume":"15","author":"Halvarsson","year":"2022","journal-title":"Parasites Vectors"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1655","DOI":"10.1017\/S003118201700227X","article-title":"Control of helminth ruminant infections by 2030","volume":"145","author":"Vercruysse","year":"2018","journal-title":"Parasitology"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Babayani, N.D., Rose Vineer, H., Walker, J.G., and Davidson, R.K. (2022). Editorial: Climate and Parasite Transmission at the Livestock-Wildlife Interface. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.816303"},{"key":"ref_4","first-page":"2359","article-title":"Strongyloides and Eimeria Infection and Its Treatment Way in Sheep","volume":"77","author":"Akhmetzhanova","year":"2022","journal-title":"Arch. Razi Inst."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Seisenov, B., Duimbayev, D., Kazhgaliyev, N., Abdrakhmanov, T., Tegza, A., Abeldinov, R., Burambayeva, N., Temirzhanova, A., Tegza, I., and Kemeshev, Z. (2023). In Vitro Fertilization in Kazakh Whiteheaded Cattle: A Comparative Study. Life, 13.","DOI":"10.3390\/life13081632"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kulpiisova, A., Yessengaliyev, K., Kassimova, G., Kozhakhmetova, A., Kadraliyeva, B., Rustem, A., Temirzhanova, A., Burambayeva, N., Chylbak-Ool, S., and Pakhomova, E. (2024). Utilization of Assisted Reproductive Technologies in Breeding Auliekol Cattle: A Comparative Study. Life, 14.","DOI":"10.3390\/life14091167"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"485","DOI":"10.5455\/OVJ.2023.v13.i4.11","article-title":"Study of the saiga helminth fauna and Ural sheep in the western region of Kazakhstan","volume":"13","author":"Kushaliyev","year":"2023","journal-title":"Open Vet. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.vetpar.2011.09.004","article-title":"Frequency distributions of helminths of wolves in Kazakhstan","volume":"184","author":"Abdybekova","year":"2012","journal-title":"Vet. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.14202\/vetworld.2024.1648-1654","article-title":"Epizootiological monitoring of wolf helminths in Northern and Central Kazakhstan","volume":"17","author":"Uakhit","year":"2024","journal-title":"Vet. World"},{"key":"ref_10","first-page":"11","article-title":"Epizootic situation on horse helminthiases in the northern region of Kazakhstan","volume":"59","author":"Zhanabayev","year":"2023","journal-title":"Vet. Ital."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhanabayev, A.A., Nurgalie, B.Y., Kereyev, A.K., Paritova, A.Y., Ussenbayev, A.Y., Bayantassova, S.M., Seitkamzina, D., Zhumagaliyeva, G.K., and Yelemessova, B. (2022). Parasite Fauna and Infection of Sheep with Parasitosis, FAO.","DOI":"10.3844\/ojbsci.2022.404.414"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1186\/s13071-021-04737-y","article-title":"Assessing the suitability of mitochondrial and nuclear DNA genetic markers for molecular systematics and species identification of helminths","volume":"14","author":"Chan","year":"2021","journal-title":"Parasites Vectors"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"108933","DOI":"10.1016\/j.vetpar.2019.108933","article-title":"Validation of ITS-2 rDNA nemabiome sequencing for ovine gastrointestinal nematodes and its application to a large scale survey of UK sheep farms","volume":"275","author":"Redman","year":"2019","journal-title":"Vet. Parasitol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ginayatov, N., Aitpayeva, Z., Zhubantayev, I., Kassymbekova, L., Zhanabayev, A., Abulgazimova, G., Arynova, R., Bizhanov, A., Mussayeva, A., and Berdikulov, M. (2025). Smallholder Cattle Farmers\u2019 Knowledge, Attitudes, and Practices Toward Rabies: A Regional Survey in Kazakhstan. Vet. Sci., 12.","DOI":"10.3390\/vetsci12040335"},{"key":"ref_15","unstructured":"Peters, W., and Pasvol, G. (2002). Tropical Medicine and Parasitology, Mosby."},{"key":"ref_16","unstructured":"Nabarro, L., Morris-Jones, S., and Moore, D. (2018). Peters\u2019 Atlas of Tropical Medicine and Parasitology E-Book, Elsevier Health Sciences."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.vetpar.2011.07.014","article-title":"Role of pet dogs and cats in the transmission of helminthic zoonoses in Europe, with a focus on echinococcosis and toxocarosis","volume":"182","author":"Deplazes","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0166-6851(92)90109-W","article-title":"Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing","volume":"54","author":"Bowles","year":"1992","journal-title":"Mol. Biochem. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1186\/s13071-015-1164-z","article-title":"Effect of gastro-intestinal nematode infection on sheep performance: A systematic review and meta-analysis","volume":"8","author":"Mavrot","year":"2015","journal-title":"Parasites Vectors"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1186\/s13071-017-2377-0","article-title":"Genetic diversity of Haemonchus contortus isolated from sympatric wild blue sheep (Pseudois nayaur) and sheep in Helan Mountains, China","volume":"10","author":"Shen","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"71","DOI":"10.2478\/macvetrev-2024-0017","article-title":"Parasites in Wildlife in the Federation of Bosnia and Herzegovina","volume":"47","author":"Kapo","year":"2024","journal-title":"Maced. Vet. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.biocon.2006.04.012","article-title":"Assessing risks of disease transmission between wildlife and livestock: The Saiga antelope as a case study","volume":"131","author":"Morgan","year":"2006","journal-title":"Biol. Conserv."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Urazayeva, S.T., Kurmangaliyeva, S.S., Kaliyev, A.A., Tussupkaliyeva, K.S., Issimov, A., Urazayeva, A.B., Tashimova, Z.K., Mussin, N.M., Begalin, T., and Amanshiyeva, A.A. (2024). Attitude toward vaccination against COVID-19 and acceptance of the national \u201cQazVac\u201d vaccine in the Aktobe city population, West Kazakhstan: A cross-sectional survey. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0303854"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Kutumbetov, L., Ragatova, A., Azanbekova, M., Myrzakhmetova, B., Aldayarov, N., Zhugunissov, K., Abduraimov, Y., Nissanova, R., Sarzhigitova, A., and Kemalova, N. (2025). Investigation of the Pathogenesis of Lumpy Skin Disease Virus in Indigenous Cattle in Kazakhstan. Pathogens, 14.","DOI":"10.3390\/pathogens14060577"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Tursunkulov, S., Zakirova, F., Moldakhmetova, Z., Tegza, A., Sayakova, Z., Abekeshev, N., Bizhanov, A., Mussayeva, A., Kanatbayev, S., and Admanova, G. (2026). Brucellosis in Kazakhstan: Knowledge, Attitudes, and Practices Among Smallholder Farmers and Veterinary Specialists. Vet. Sci., 13.","DOI":"10.3390\/vetsci13020191"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"338","DOI":"10.14202\/vetworld.2020.338-344","article-title":"Helminth infections of great concern among cattle in Nigeria: Insight to its prevalence, species diversity, patterns of infections and risk factors","volume":"13","author":"Ganiyu","year":"2020","journal-title":"Vet. World"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.cvfa.2019.12.001","article-title":"Biology, Epidemiology, Diagnosis, and Management of Anthelmintic Resistance in Gastrointestinal Nematodes of Livestock","volume":"36","author":"Kaplan","year":"2020","journal-title":"Vet. Clin. N. Am. Food Anim. Pract."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Kulpiisova, A., Dikhanbayeva, F., Tegza, A., Tegza, I., Abzhanova, S., Moldakhmetova, Z., Uazhanova, R., Alikhanov, K., Yerzhigitov, Y., and Shambulova, G. (2025). Assessment of food safety awareness and hygiene practices among food handlers in Almaty, Kazakhstan. BMC Public Health, 25.","DOI":"10.1186\/s12889-025-24124-x"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1051\/parasite\/2020062","article-title":"Increasing importance of anthelmintic resistance in European livestock: Creation and meta-analysis of an open database","volume":"27","author":"Vineer","year":"2020","journal-title":"Parasite"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"32","DOI":"10.31016\/1998-8435-2020-14-2-32-37","article-title":"Helminth Contamination of Wild Ruminants in the West Kazakhstan Region and Activity of the Supramolecular Complex of Albendazole with Polyvinylpyrrolidone at Helminthoses","volume":"14","author":"Karmaliev","year":"2020","journal-title":"Russ. J. Parasitol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/5\/550\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T09:47:18Z","timestamp":1779270438000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/5\/550"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,20]]},"references-count":30,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["pathogens15050550"],"URL":"https:\/\/doi.org\/10.3390\/pathogens15050550","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,20]]}}}