{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T09:06:04Z","timestamp":1779786364975,"version":"3.53.1"},"reference-count":23,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>In 2023, ASF was recognized in Lombardy in wild boars, but shortly thereafter the disease was also identified in pig farms, with serious economic repercussions for the entire national pig sector. To identify factors related to the introduction of the infection into pig farms, a case\u2013control study was conducted with the aim of gaining knowledge on the risk and protective factors involved in the introduction of the ASF virus into intensive pig farms. To this end, a questionnaire was developed on risk factors related to ASFV transmission into pig farms and on good management and biosecurity practices. The results of the study showed that measures aimed at strengthening the segregation of the farm from the external environment (external biosecurity), such as the mandatory passage through a hygiene lock upon entry, the presence of a hygiene lock for farm personnel and external visitors, the presence of special equipment and disinfection points at the entrance to the sheds, the loading of dead pigs outside the animal housing area and the ownership of non-adjacent agricultural land, were associated with a reduced risk. This information, if effectively communicated, could be of direct practical value to farmers to ensure the successful implementation of farm biosecurity.<\/jats:p>","DOI":"10.3390\/pathogens15060569","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T08:16:08Z","timestamp":1779783368000},"page":"569","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["African Swine Fever (ASF): A Study to Identify Risk Factors Associated with the Introduction of the Disease into Pig Farms"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0061-7724","authenticated-orcid":false,"given":"Silvia","family":"Bellini","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia-Romagna, Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3427-722X","authenticated-orcid":false,"given":"Alessandra","family":"Scaburri","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia-Romagna, Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7544-2693","authenticated-orcid":false,"given":"Matteo","family":"Tonni","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia-Romagna, Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Maggiano","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia-Romagna, Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8073-4968","authenticated-orcid":false,"given":"Sara","family":"Fusar Poli","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia-Romagna, Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Bernardis","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia-Romagna, Via A. Bianchi 9, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Santucci","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia-Romagna, Via A. 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Pathogens, 10.","DOI":"10.3390\/pathogens10010084"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Animal Health and Animal Welfare (AHAW) (2014). Scientific Opinion on African swine fever. EFSA J., 12, 3628.","DOI":"10.2903\/j.efsa.2014.3628"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA), Boklund, A.E., St\u00e5hl, K., Miranda Chueca, M.A., Podg\u00f3rski, T., Vergne, T., Corti\u00f1as Abrahantes, J., Cattaneo, E., Dhollander, S., and Papanikolaou, A. (2024). Risk and protective factors for ASF in domestic pigs and wild boar in the EU, and mitigation measures for managing the disease in wild boar. EFSA J., 22, e9095.","DOI":"10.2903\/j.efsa.2024.9095"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13081","DOI":"10.1038\/s41598-021-92593-2","article-title":"The first genotype II African swine fever virus isolated in Africa provides insight into the current Eurasian pandemic","volume":"11","author":"Njau","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Cwynar, P., Stojkov, J., and Wlazlak, K. (2019). African Swine Fever Status in Europe. Viruses, 11.","DOI":"10.3390\/v11040310"},{"key":"ref_7","unstructured":"(2026, January 29). World Animal Health Information System (WOAH). Available online: https:\/\/wahis.woah.org\/#\/home."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Bellini, S., Salvato, S., Bernardis, M., Chiari, M., Martinello, F., Galimberti, L., Maggiano, V., Feliziani, F., Farioli, M., and Alborali, G.L. (2025). Epidemiological Characteristics of the African Swine Fever Genotype II Epidemic in Domestic Pigs in Lombardy (Northern Italy) in 2023 and 2024. Viruses, 17.","DOI":"10.3390\/v17030327"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8850856","DOI":"10.1155\/tbed\/8850856","article-title":"Risk Mapping of African Swine Fever in Domestic Pigs and Wild Boars to Enhance Management and Surveillance in Asia","volume":"2025","author":"Kawaguchi","year":"2025","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Bellini, S. (2021). 7. The pig sector in the European Union. Understanding and Combatting African Swine Fever, Wageningen Academic.","DOI":"10.3920\/978-90-8686-910-7_7"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA), Boklund, A., Cay, B., Depner, K., F\u00f6ldi, Z., Guberti, V., Masiulis, M., Miteva, A., More, S., and Olsevskis, E. (2018). Epidemiological analyses of African swine fever in the European Union (November 2017 until November 2018). EFSA J., 16, e05494.","DOI":"10.2903\/j.efsa.2018.5494"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA), Ba\u00f1os, J.V., Boklund, A., Gogin, A., Gort\u00e1zar, C., Guberti, V., Helyes, G., Kantere, M., Korytarova, D., and Linden, A. (2021). Epidemiological analysis of African swine fever in the European Union (September 2019 to August 2020). EFSA J., 19, e06572.","DOI":"10.2903\/j.efsa.2021.6572"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s13028-016-0264-x","article-title":"Preventive measures aimed at minimizing the risk of African swine fever virus spread in pig farming systems","volume":"58","author":"Bellini","year":"2016","journal-title":"Acta Vet. Scand."},{"key":"ref_14","unstructured":"Unit for Veterinary Epidemiology UGent (2021). Biocheck UGent, Faculty of Veterinary Medicine, Ghent University."},{"key":"ref_15","unstructured":"(2025, July 03). ClassyFarm IZSLER and Italian Ministry of Health. Available online: https:\/\/www.classyfarm.it\/."},{"key":"ref_16","unstructured":"Italian Ministry of Health (2022). National Decree of 28 June 2022, No. 173."},{"key":"ref_17","unstructured":"World Animal Health Information System (WOAHS) (2024, December 20). Terrestrial Animal Health Code. Chapter 15.1\u2014Infection with African Swine Fever Virus. Available online: https:\/\/www.woah.org\/fileadmin\/Home\/eng\/Health_standards\/tahm\/3.09.01_ASF.pdf."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1111\/tbed.12391","article-title":"Statistical Exploration of Local Transmission Routes for African Swine Fever in Pigs in the Russian Federation, 2007\u20132014","volume":"64","author":"Vergne","year":"2017","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.3201\/eid2707.204230","article-title":"Transmission Dynamics of African Swine Fever Virus, South Korea, 2019","volume":"27","author":"Yoo","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10215","DOI":"10.1038\/s41598-020-66381-3","article-title":"Risk factors for African swine fever incursion in Romanian domestic farms during 2019","volume":"10","author":"Boklund","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_21","unstructured":"R Core Team (2018). R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https:\/\/www.R-project.org\/."},{"key":"ref_22","unstructured":"Open-Source Geospatial Foundation (2009). Quantum GIS Software, Open-Source Geospatial Foundation. Available online: https:\/\/www.qgis.org."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"105962","DOI":"10.1016\/j.prevetmed.2023.105962","article-title":"Estimating the effectiveness of control actions on African swine fever transmission in commercial swine populations in the United States","volume":"217","author":"Sykes","year":"2023","journal-title":"Prev. Vet. 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