{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:14:10Z","timestamp":1785413650885,"version":"3.56.0"},"reference-count":60,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["R01GM120108"],"award-info":[{"award-number":["R01GM120108"]}]},{"award":["R01GM120108"],"award-info":[{"award-number":["R01GM120108"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"USDA National Institute of Food and Agriculture","doi-asserted-by":"crossref","award":["1007272"],"award-info":[{"award-number":["1007272"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"crossref"}]},{"award":["1007272"],"award-info":[{"award-number":["1007272"]}],"id":[{"id":"https:\/\/ror.org\/05qx3fv49","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Ministry of Higher Education of the Arab Republic of Egypt","award":["GM 1147"],"award-info":[{"award-number":["GM 1147"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Potyvirus rapae (turnip mosaic virus, TuMV) is widely used as a model system in plant\u2013virus interaction studies. The TuMV RNA genome encodes 11 proteins, some of which remain poorly characterized, while the functions of others are well defined. Studying individual proteins in isolation may not recapitulate native expression levels, subcellular localization, and interaction with host factors during virus replication and movement. An alternative approach is to tag individual viral proteins in the context of an infectious clone. Epitope tags may alter protein functions and affect viral replication, movement, or a combination of essential steps, thus leading to changes in pathogenicity. Because they have central roles in viral infection, here we measured the effect of individually tagging the helper component proteinase (HC-Pro) and nuclear inclusion protein b (NIb) with a 6His-3xFLAG tag. Epitope tags were placed at the N-terminus of HC-Pro and the N- and C-termini of NIb within a TuMV infectious clone carrying coding sequences for the green fluorescent protein (TuMV-GFP). Constructs carrying a tagged HC-Pro displayed pathogenicity similar to that observed for TuMV-GFP in Nicotiana benthamiana and Arabidopsis thaliana plants. In contrast, infectivity of NIb-tagged clones became temperature sensitive and, even at the permissive temperature, showed reduced pathogenicity compared to TuMV-GFP. Providing a silencing suppressor in trans did not restore infection efficiency, suggesting reduced viral fitness due to structural or functional disruption caused by the epitope tags. Structural models generated using AlphaFold2 showed no effect of the tag on HC-Pro. In contrast, structural models illustrated tag interference with the NIb catalytic site. AlphaFold2 was further used to predict the structural impact of several tags on NIb and to predict the effect of a 6HIS-3xFlag tag on all other TuMV proteins. This study provides a broadly applicable framework for selecting suitable epitope tags to mark viral proteins and maintain function in the context of virus infection.<\/jats:p>","DOI":"10.3390\/pathogens15060611","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T10:04:14Z","timestamp":1780913054000},"page":"611","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Contrasting Effects of Tagging Turnip Mosaic Virus Proteins"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7309-5229","authenticated-orcid":false,"given":"Amany E.","family":"Gomaa","sequence":"first","affiliation":[{"name":"Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA"},{"name":"Department of Botany, Faculty of Science, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Parperides","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2706-2028","authenticated-orcid":false,"given":"Xin-Qiu","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Nebraska-Omaha, Omaha, NE 68182, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Espinoza Vergara","sequence":"additional","affiliation":[{"name":"Departamento de Parasitolog\u00eda Agr\u00edcola, Universidad Aut\u00f3noma Chapingo, Texcoco 56230, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziomara","family":"Jurado","sequence":"additional","affiliation":[{"name":"Biology Department, Nebraska Wesleyan University, Lincoln, NE 68504, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4681-470X","authenticated-orcid":false,"given":"Hernan","family":"Garcia-Ruiz","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1146\/annurev-phyto-020620-114550","article-title":"Research advances in potyviruses: From the laboratory bench to the field","volume":"59","author":"Yang","year":"2021","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.coviro.2021.03.002","article-title":"Cell-to-cell movement of plant viruses via plasmodesmata: A current perspective on potyviruses","volume":"48","author":"Wang","year":"2021","journal-title":"Curr. Opin. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"10460","DOI":"10.1128\/JVI.00819-09","article-title":"Turnip mosaic virus RNA replication complex vesicles are mobile, align with microfilaments, and are each derived from a single viral genome","volume":"83","author":"Cotton","year":"2009","journal-title":"J. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Suman, R., Rishi, N., and Chandel, V. (2023). Molecular approaches in plant host-virus interaction studies. Plant RNA Viruses, Elsevier.","DOI":"10.1016\/B978-0-323-95339-9.00003-X"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1073\/pnas.1910787117","article-title":"Rescue of tomato spotted wilt virus entirely from complementary DNA clones","volume":"117","author":"Feng","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1105\/tpc.109.073056","article-title":"Arabidopsis RNA-dependent RNA polymerases and dicer-like proteins in antiviral defense and small interfering RNA biogenesis during turnip mosaic virus infection","volume":"22","author":"Takeda","year":"2010","journal-title":"Plant Cell"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7066","DOI":"10.1073\/pnas.81.22.7066","article-title":"Multicomponent RNA plant virus infection derived from cloned viral cDNA","volume":"81","author":"Ahlquist","year":"1984","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"117282","DOI":"10.1016\/j.celrep.2026.117282","article-title":"Low expression levels of the conserved nuclear histone acetyltransferase 1 restrict virus infection in plants","volume":"45","author":"Dong","year":"2026","journal-title":"Cell Rep."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Li, Y., Yang, X., Wen, Z., Nagalakshmi, U., and Dinesh-Kumar, S.P. (2020). Turboid-based proximity labeling for in planta identification of protein-protein interaction networks. J. Vis. Exp., e60728.","DOI":"10.3791\/60728-v"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Martinez, F., Rodrigo, G., Aragones, V., Ruiz, M., Lodewijk, I., Fernandez, U., Elena, S.F., and Daros, J.A. (2016). Interaction network of tobacco etch potyvirus NIa protein with the host proteome during infection. BMC Genom., 17.","DOI":"10.1186\/s12864-016-2394-y"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Arakawa, T., and Akuta, T. (2025). Beyond purification: Evolving roles of fusion tags in biotechnology. Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47090768"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Garcia-Ruiz, H., Carbonell, A., Hoyer, J.S., Fahlgren, N., Gilbert, K.B., Takeda, A., Giampetruzzi, A., Garcia Ruiz, M.T., McGinn, M.G., and Lowery, N. (2015). Roles and programming of arabidopsis ARGONAUTE proteins during turnip mosaic virus infection. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004755"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s43556-025-00266-7","article-title":"Development of viral infectious clones and their applications based on yeast and bacterial artificial chromosome platforms","volume":"6","author":"Wu","year":"2025","journal-title":"Mol. Biomed."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Wang, R., Cao, X., Lu, K., Chang, Z., Dong, X., Guo, H., Wei, X., Dang, R., Wang, J., and Wang, X. (2024). Rescuing Newcastle disease virus with tag for screening viral-host interacting proteins based on highly efficient reverse genetics. Front. Vet. Sci., 11.","DOI":"10.3389\/fvets.2024.1418760"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"581093","DOI":"10.1155\/2013\/581093","article-title":"Several affinity tags commonly used in chromatographic purification","volume":"2013","author":"Zhao","year":"2013","journal-title":"J. Anal. Methods Chem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1038\/nbt.4201","article-title":"Efficient proximity labeling in living cells and organisms with TurboID","volume":"36","author":"Branon","year":"2018","journal-title":"Nat. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1586\/14789450.2016.1147353","article-title":"Proteomic approaches to uncovering virus\u2013host protein interactions during the progression of viral infection","volume":"13","author":"Lum","year":"2016","journal-title":"Expert Rev. Proteom."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Zarzy\u0144ska-Nowak, A., Minicka, J., Wieczorek, P., and Hasi\u00f3w-Jaroszewska, B. (2024). Development of stable infectious cDNA clones of tomato black ring virus tagged with green fluorescent protein. Viruses, 16.","DOI":"10.3390\/v16010125"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Garcia-Ruiz, H., Gabriel Peralta, S.M., and Harte-Maxwell, P.A. (2018). Tomato spotted wilt virus NSs protein supports infection and systemic movement of a potyvirus and is a symptom determinant. Viruses, 10.","DOI":"10.3390\/v10030129"},{"key":"ref_20","first-page":"389","article-title":"Gene tagging strategies to assess protein expression, localization, and function in Drosophila","volume":"207","author":"Kanca","year":"2017","journal-title":"Genetics"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Merleau, N.S.C., P\u00e9nisson, S., Gerrish, P.J., Elena, S.F., and Smerlak, M. (2021). Why are viral genomes so fragile? The bottleneck hypothesis. PLoS Comput. Biol., 17.","DOI":"10.1101\/2021.01.23.427881"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1016\/j.jmb.2018.12.004","article-title":"Assessment of GFP tag position on protein localization and growth fitness in yeast","volume":"431","author":"Weill","year":"2019","journal-title":"J. Mol. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"9106","DOI":"10.1039\/C8CS00537K","article-title":"Recent progress in enzymatic protein labelling techniques and their applications","volume":"47","author":"Zhang","year":"2018","journal-title":"Chem. Soc. Rev."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s00253-002-1158-6","article-title":"Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems","volume":"60","author":"Terpe","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1093\/plphys\/kiab479","article-title":"Advances in enzyme-mediated proximity labeling and its potential for plant research","volume":"188","author":"Mair","year":"2022","journal-title":"Plant Physiol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"H\u00fdskov\u00e1, V., B\u011blono\u017en\u00edkov\u00e1, K., Chmel\u00edk, J., Hoffmeisterov\u00e1, H., \u010ce\u0159ovsk\u00e1, N., Moravec, T., and Ry\u0161lav\u00e1, H. (2024). Potyviral helper-component protease: Multifaced functions and interactions with host proteins. Plants, 13.","DOI":"10.3390\/plants13091236"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1111\/mpp.12553","article-title":"The HCPro from the potyviridae family: An enviable multitasking helper component that every virus would like to have","volume":"19","author":"Valli","year":"2018","journal-title":"Mol. Plant Pathol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Tu, Y., Zhang, Z., Li, D., Li, H., Dong, J., and Wang, T. (2015). Potato virus Y HC-Pro reduces the ATPase activity of NtMinD, which results in enlarged chloroplasts in HC-Pro transgenic tobacco. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0136210"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1099\/vir.0.81107-0","article-title":"HcPro, a multifunctional protein encoded by a plant RNA virus, targets the 20S proteasome and affects its enzymic activities","volume":"86","author":"Ballut","year":"2005","journal-title":"J. Gen. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ocampo Ocampo, T., Gabriel Peralta, S.M., Bacheller, N., Uiterwaal, S., Knapp, A., Hennen, A., Ochoa-Martinez, D.L., and Garcia-Ruiz, H. (2016). Antiviral RNA silencing suppression activity of Tomato spotted wilt virus NSs protein. Genet. Mol. Res., 15, Available online: https:\/\/pubmed.ncbi.nlm.nih.gov\/27323202\/ (accessed on 3 May 2026).","DOI":"10.4238\/gmr.15028625"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Shen, W., Shi, Y., Dai, Z., and Wang, A. (2020). The RNA-dependent RNA polymerase NIb of potyviruses plays multifunctional, contrasting roles during viral infection. Viruses, 12.","DOI":"10.3390\/v12010077"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Venkataraman, S., Prasad, B.V., and Selvarajan, R. (2018). RNA dependent RNA polymerases: Insights from structure, function and evolution. Viruses, 10.","DOI":"10.3390\/v10020076"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Zhang, M., Gong, P., Ge, L., Li, Y., Chang, Z., Qiao, R., Zhou, X., Wang, A., and Li, F. (2022). Nuclear exportin 1 (XPO1) binds to the nuclear localization\/export signal of the turnip mosaic virus NIb to promote viral infection. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.780724"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1104\/pp.103.027979","article-title":"A gateway cloning vector set for high-throughput functional analysis of genes in planta","volume":"133","author":"Curtis","year":"2003","journal-title":"Plant Physiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Huovinen, T., Brockmann, E.C., Akter, S., Perez-Gamarra, S., Yl\u00e4-Pelto, J., Liu, Y., and Lamminm\u00e4ki, U. (2012). Primer extension mutagenesis powered by selective rolling circle amplification. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0031817"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1126\/science.aad3436","article-title":"Using decoys to expand the recognition specificity of a plant disease resistance protein","volume":"351","author":"Kim","year":"2016","journal-title":"Science"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3507","DOI":"10.1038\/emboj.2010.215","article-title":"Plant virus-mediated induction of miR168 is associated with repression of ARGONAUTE1 accumulation","volume":"29","author":"Agyi","year":"2010","journal-title":"EMBO J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/pro.3943","article-title":"UCSF ChimeraX: Structure visualization for researchers, educators, and developers","volume":"30","author":"Pettersen","year":"2021","journal-title":"Protein Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3084","DOI":"10.1021\/ct400341p","article-title":"PTRAJ and CPPTRAJ: Software for processing and analysis of molecular dynamics trajectory data","volume":"9","author":"Roe","year":"2013","journal-title":"J. Chem. Theory Comput."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"W368","DOI":"10.1093\/nar\/gky309","article-title":"MOLEonline: A web-based tool for analyzing channels, tunnels and pores (2018 update)","volume":"46","author":"Pravda","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Pasin, F., Simon-Mateo, C., and Garc\u00eda, J.A. (2014). The hypervariable amino-terminus of P1 protease modulates potyviral replication and host defense responses. PLoS Pathog., 10.","DOI":"10.1371\/journal.ppat.1003985"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5119","DOI":"10.1128\/JVI.00024-16","article-title":"Plum pox virus 6K1 protein is required for viral replication and targets the viral replication complex at the early stage of infection","volume":"90","author":"Cui","year":"2016","journal-title":"J. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Bera, S., Arena, G.D., Ray, S., Flannigan, S., and Casteel, C.L. (2022). The potyviral protein 6K1 reduces plant proteases activity during turnip mosaic virus infection. Viruses, 14.","DOI":"10.3390\/v14061341"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1186\/s12985-015-0369-2","article-title":"The multifunctional protein CI of potyviruses plays interlinked and distinct roles in viral genome replication and intercellular movement","volume":"12","author":"Deng","year":"2015","journal-title":"Virol. J."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6695","DOI":"10.1128\/JVI.00503-15","article-title":"The vesicle-forming 6K2 protein of turnip mosaic virus interacts with the COPII coatomer sec24a for viral systemic infection","volume":"89","author":"Jiang","year":"2015","journal-title":"J. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"24056","DOI":"10.1073\/pnas.1904752116","article-title":"Structural studies of the eIF4E\u2013VPg complex reveal a direct competition for capped RNA: Implications for translation","volume":"116","author":"Volpon","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s10059-000-0505-7","article-title":"Characterization of active-site residues of the nia protease from tobacco vein mottling virus","volume":"10","author":"Hwang","year":"2000","journal-title":"Mol. Cells"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"e01444-21","DOI":"10.1128\/JVI.01444-21","article-title":"Proteolytic processing of plant proteins by potyvirus NIa proteases","volume":"96","author":"Xiao","year":"2022","journal-title":"J. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1111\/mpp.12973","article-title":"The cis-expression of the coat protein of turnip mosaic virus is essential for viral intercellular movement in plants","volume":"21","author":"Dai","year":"2020","journal-title":"Mol. Plant Pathol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"111","DOI":"10.7171\/jbt.17-2803-002","article-title":"Evaluation of a bead-free coimmunoprecipitation technique for identification of virus\u2013host protein interactions using high-resolution mass spectrometry","volume":"28","author":"DeBlasio","year":"2017","journal-title":"J. Biomol. Tech."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Niraula, P.M., Howell, S., Stratton, C.A., Moore, M.T., Dopler, M.B., Abeer, M.I., Gitcho, M.A., and Fondong, V.N. (2026). Potato virus Y NIb multifunctional protein suppresses antiviral defense by interacting with several protein components of the RNA silencing pathway. Int. J. Mol. Sci., 27.","DOI":"10.20944\/preprints202601.0554.v1"},{"key":"ref_53","unstructured":"Holste, N.M. (2020). Polerovirus Genomic Variation and Mechanisms of Silencing Suppression by p0 Protein. [Master\u2019s Thesis, School of Biological Sciences, University of Nebraska-Lincoln]."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"jkaf070","DOI":"10.1093\/g3journal\/jkaf070","article-title":"Multimerized epitope tags for high-sensitivity protein detection","volume":"15","author":"Stowers","year":"2025","journal-title":"G3 Genes Genomes Genet."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1038\/nature10182","article-title":"The ELF4-ELF3-LUX complex links the circadian clock to diurnal control of hypocotyl growth","volume":"475","author":"Nusinow","year":"2011","journal-title":"Nature"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Duflos, C., and Michiels, T. (2023). Regulation of viral RNA-dependent RNA polymerases by phosphorylation. Front. Virol., 3.","DOI":"10.3389\/fviro.2023.1176840"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1038\/s41477-025-01940-y","article-title":"Structural basis for the activation of plant bunyavirus replication machinery and its dual-targeted inhibition by ribavirin","volume":"11","author":"Li","year":"2025","journal-title":"Nat. Plants"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1038\/s41467-024-55288-6","article-title":"Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection","volume":"16","author":"Ge","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Qin, L., Wang, X., Dai, Z., Shen, W., Li, F., Wang, A., Valli, A.A., and Cui, H. (2025). Natural self-attenuation of pathogenic viruses by deleting the silencing suppressor coding sequence for long-term plant-virus coexistence. PLoS Pathog., 21.","DOI":"10.1101\/2025.02.28.640734"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1104\/pp.17.01198","article-title":"Turnip mosaic virus counteracts selective autophagy of the viral silencing suppressor HCpro","volume":"176","author":"Hafren","year":"2018","journal-title":"Plant Physiol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/6\/611\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T04:23:58Z","timestamp":1781065438000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/6\/611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,8]]},"references-count":60,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["pathogens15060611"],"URL":"https:\/\/doi.org\/10.3390\/pathogens15060611","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,8]]}}}