{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:55:34Z","timestamp":1785412534646,"version":"3.56.0"},"reference-count":34,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["1R21AI51483"],"award-info":[{"award-number":["1R21AI51483"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"award":["1R21AI51483"],"award-info":[{"award-number":["1R21AI51483"]}],"id":[{"id":"https:\/\/ror.org\/043z4tv69","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Orthoebolavirus causes severe Ebola virus disease (EVD) and deadly outbreaks in humans. This infection occurs through macropinocytosis and trafficking to late endosomes or lysosomes that utilize the receptor Niemann\u2013Pick C1 (NPC1) to enter the cell cytoplasm. We designed peptide inhibitors based on the NPC1 receptor to target the NPC1 binding site to block viral entry. The results indicated that the ligand-based peptide inhibitors showed potent inhibition activities in vitro studies against pseudotyped or replication-competent Orthoebolavirus. Therefore, we further evaluated one of them in a mouse model challenged with mice-adapted Ebola viruses, which showed some protection efficacy compared with the control group. This study suggests that ligand-based peptides are encouraging inhibitors in the development of inhibitors against Ebola virus infection.<\/jats:p>","DOI":"10.3390\/pathogens15060640","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T09:06:52Z","timestamp":1781600812000},"page":"640","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of Peptide Entry Inhibitors Targeting the Endosomal Receptor NPC1 Binding Site of Orthoebolavirus"],"prefix":"10.3390","volume":"15","author":[{"given":"Leah Liu","family":"Wang","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine and Biomedical Sciences, Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6031-5360","authenticated-orcid":false,"given":"Kendra","family":"Alfson","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute, 8715 W. Military Drive, San Antonio, TX 78227, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9770-7525","authenticated-orcid":false,"given":"J. J.","family":"Patten","sequence":"additional","affiliation":[{"name":"National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc E.","family":"Mattix","sequence":"additional","affiliation":[{"name":"Nonclinical Pathology Services, LLC, 5920 Clubhouse Pointe Dr., Medina, OH 44256, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yenny","family":"Goez-Gazi","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute, 8715 W. Military Drive, San Antonio, TX 78227, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0935-6455","authenticated-orcid":false,"given":"Sean N.","family":"Avedissian","sequence":"additional","affiliation":[{"name":"College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9168-2892","authenticated-orcid":false,"given":"Robert A.","family":"Davey","sequence":"additional","affiliation":[{"name":"National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7046-7683","authenticated-orcid":false,"suffix":"Jr.","given":"Ricardo","family":"Carrion","sequence":"additional","affiliation":[{"name":"Texas Biomedical Research Institute, 8715 W. Military Drive, San Antonio, TX 78227, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5656-5698","authenticated-orcid":false,"given":"Shi-Hua","family":"Xiang","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Biomedical Sciences, Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68588, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1007\/s00705-023-05834-2","article-title":"Renaming of genera Ebolavirus and Marburgvirus to Orthoebolavirus and Orthomarburgvirus, respectively, and introduction of binomial species names within family Filoviridae","volume":"168","author":"Biedenkopf","year":"2023","journal-title":"Arch. 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