{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:43:10Z","timestamp":1791909790255,"version":"4.3.4"},"reference-count":26,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T00:00:00Z","timestamp":1782086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technology, and Innovation"},{"id":[{"id":"https:\/\/ror.org\/050zdnc69","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["403761\/2020-4"],"award-info":[{"award-number":["403761\/2020-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["400172\/2022-4"],"award-info":[{"award-number":["400172\/2022-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"award":["403761\/2020-4"],"award-info":[{"award-number":["403761\/2020-4"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"award":["400172\/2022-4"],"award-info":[{"award-number":["400172\/2022-4"]}],"id":[{"id":"https:\/\/ror.org\/03swz6y49","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["2022.10212.BD"],"award-info":[{"award-number":["2022.10212.BD"]}]},{"award":["2022.10212.BD"],"award-info":[{"award-number":["2022.10212.BD"]}],"id":[{"id":"https:\/\/ror.org\/00snfqn58","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>In this study, we conducted a molecular investigation of hemotropic Mycoplasma spp. in bat species captured in the northern region of the Pantanal biome, Mato Grosso State, Brazil. Tissue samples were screened by qPCR targeting the 16S rRNA gene. Positive samples were subsequently subjected to conventional PCR assays targeting partial fragments of the 16S rRNA (~900 bp) and 23S rRNA (~800 bp) genes. Hemoplasma DNA was detected in four bat species: Glossophaga soricina, Molossops temminckii, Molossus rufus, and Desmodus rotundus. Phylogenetic analyses based on partial 16S and 23S rRNA gene sequences demonstrated that the detected hemoplasmas clustered predominantly with previously described bat-associated hemoplasmas from Brazil and other countries in the Americas. Notably, the detection in M. temminckii represents, to our knowledge, the first molecular evidence of hemotropic Mycoplasma infection in this bat species. These findings expand current knowledge regarding the occurrence, host range, and genetic diversity of hemotropic Mycoplasma spp. in bats from the Pantanal biome and contribute to wildlife surveillance efforts in this ecologically important region.<\/jats:p>","DOI":"10.3390\/pathogens15060654","type":"journal-article","created":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T07:57:24Z","timestamp":1782287844000},"page":"654","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Characterization of Hemotropic Mycoplasma spp. From Bats (Chiroptera) in the Northern Pantanal, Brazil"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4045-9073","authenticated-orcid":false,"given":"Nathalia de Assis","family":"Pereira","sequence":"first","affiliation":[{"name":"Laboratory of Virology and Rickettsiosis, Faculty of Veterinary Medicine, Federal University of Mato Grosso State, Cuiab\u00e1 78060-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane","family":"Saldanha","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Rickettsiosis, Faculty of Veterinary Medicine, Federal University of Mato Grosso State, Cuiab\u00e1 78060-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4379-5993","authenticated-orcid":false,"given":"Thiago Borges Fernandes","family":"Semedo","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Rickettsiosis, Faculty of Veterinary Medicine, Federal University of Mato Grosso State, Cuiab\u00e1 78060-900, Brazil"},{"name":"CIBIO, Centro de Investigac\u00e3o em Biodiversidade e Recursos Gen\u00e9ticos, Universidade do Porto, 4099-002 Porto, Portugal"},{"name":"Departamento de Biologia, Universidade do Porto, 4099-002 Porto, Portugal"},{"name":"BIOPOLIS Program in Genomics, Biodiversity and Land Planning, 4099-002 Porto, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1701-5930","authenticated-orcid":false,"given":"Guilherme Siniciato Terra","family":"Garbino","sequence":"additional","affiliation":[{"name":"Museu de Zoologia Jo\u00e3o Moojen, Departamento de Biologia Animal, Universidade Federal de Vicosa, Vi\u00e7osa 36570-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2353-5000","authenticated-orcid":false,"given":"Rog\u00e9rio Vieira","family":"Rossi","sequence":"additional","affiliation":[{"name":"Laboratory of Mastozoology, BioScience Institute, Federal University of Mato Grosso State, Cuiab\u00e1 78060-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5467-9591","authenticated-orcid":false,"given":"Sofia de Souza Pereira","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Rickettsiosis, Faculty of Veterinary Medicine, Federal University of Mato Grosso State, Cuiab\u00e1 78060-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayanne Luns","family":"Hatum","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Rickettsiosis, Faculty of Veterinary Medicine, Federal University of Mato Grosso State, Cuiab\u00e1 78060-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8631-2712","authenticated-orcid":false,"given":"Th\u00e1llitha Samih Wischral Jayme","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6613-0287","authenticated-orcid":false,"given":"Rafael Felipe da Costa","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Community Health, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA"},{"name":"Center for Computational Intelligence to Predict Environment and Health Risks, The University of North Carolina at Charlotte, Charlotte, NC 28223, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7250-3024","authenticated-orcid":false,"given":"Jansen","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Emerging Viruses Research and Clinical and Molecular Virology Laboratories, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4898-6553","authenticated-orcid":false,"given":"Edison Luiz","family":"Durigon","sequence":"additional","affiliation":[{"name":"Emerging Viruses Research and Clinical and Molecular Virology Laboratories, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"},{"name":"Institut Pasteur of S\u00e3o Paulo, S\u00e3o Paulo 05508-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8631-522X","authenticated-orcid":false,"given":"Daniel Moura","family":"de Aguiar","sequence":"additional","affiliation":[{"name":"Laboratory of Virology and Rickettsiosis, Faculty of Veterinary Medicine, Federal University of Mato Grosso State, Cuiab\u00e1 78060-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,22]]},"reference":[{"key":"ref_1","unstructured":"Kunz, T.H., and Fenton, M.B. 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