{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:51:02Z","timestamp":1785433862032,"version":"3.56.0"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004539","name":"Kasetsart University","doi-asserted-by":"publisher","award":["RA plus 67\/12"],"award-info":[{"award-number":["RA plus 67\/12"]}],"id":[{"id":"10.13039\/501100004539","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004539","name":"Kasetsart University","doi-asserted-by":"publisher","award":["VET.KU2025-TWP.05"],"award-info":[{"award-number":["VET.KU2025-TWP.05"]}],"id":[{"id":"10.13039\/501100004539","id-type":"DOI","asserted-by":"publisher"}]},{"award":["RA plus 67\/12"],"award-info":[{"award-number":["RA plus 67\/12"]}],"id":[{"id":"https:\/\/ror.org\/05gzceg21","id-type":"ROR","asserted-by":"publisher"}]},{"award":["VET.KU2025-TWP.05"],"award-info":[{"award-number":["VET.KU2025-TWP.05"]}],"id":[{"id":"https:\/\/ror.org\/05gzceg21","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Canine vector-borne pathogens (CVBPs) pose a major challenge in shelter medicine, yet data from shelter populations in Vietnam remain unknown. This study determined the prevalence, and risk factors of CVBPs in shelter dogs in northern Vietnam. Blood samples from 300 apparently healthy dogs from three shelters in Hanoi were screened by PCR for Babesia vogeli, Hepatozoon canis, Rickettsia spp., and Mycoplasma spp. Representative positive amplicons underwent Sanger sequencing and BLAST analysis. Sequence analysis showed 96.07\u2013100% identity with reference strains, with phylogenetic trees confirming clustering within B. vogeli, H. canis, M. haemocanis and R. felis clades. Overall, 43.7% (131\/300) of dogs were infected with at least one pathogen, with shelter-level prevalence ranging from 38.0 to 52.0%. Single infections accounted for 35.0%, dominated by R. felis (25.7%) and M. haemocanis (24.0%), B. vogeli and H. canis were low (1.3% each). Co-infections were found in 8.7% of dogs, primarily R. felis and M. haemocanis (8.3%). No evaluated host factors (age, sex, breed, body size, housing style) significantly associated with infection (p &gt; 0.05). This study provides the first molecular evidence of canine vector-borne pathogen circulation in Vietnamese shelter dogs, emphasizing the need of ectoparasite control and One Health-oriented surveillance.<\/jats:p>","DOI":"10.3390\/pathogens15070702","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T15:52:24Z","timestamp":1783007544000},"page":"702","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular-Based Detection of Vector-Borne Diseases in Shelter Dogs in Northern of Vietnam"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9693-1915","authenticated-orcid":false,"given":"Bach Xuan","family":"Pham","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"},{"name":"Graduate Program in Animal Health and Biomedical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linh Khanh","family":"Bui","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 12406, Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5982-4811","authenticated-orcid":false,"given":"Tawin","family":"Inpankaew","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.pt.2009.01.003","article-title":"Managing canine vector-borne diseases of zoonotic concern: Part one","volume":"25","author":"Otranto","year":"2009","journal-title":"Trends Parasitol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1186\/s13071-023-05994-9","article-title":"Association between vector-borne pathogen seroprevalence in shelter-housed and owned dog populations in the contiguous United States of America","volume":"16","author":"Gettings","year":"2023","journal-title":"Parasites Vectors"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1186\/s13071-023-05813-1","article-title":"Spatial and risk factor analyses of vector-borne pathogens among shelter dogs in the Eastern United States","volume":"16","author":"Hazelrig","year":"2023","journal-title":"Parasites Vectors"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100112","DOI":"10.1016\/j.crpvbd.2022.100112","article-title":"Geographical analysis of seroprevalence of Ehrlichia spp., Anaplasma spp., Borrelia burgdorferi and Dirofilaria immitis, in clinics and dog shelters in different Mexican states","volume":"3","author":"Bedoya","year":"2022","journal-title":"Curr. 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