{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T05:00:44Z","timestamp":1785042044222,"version":"3.55.0"},"reference-count":28,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and ICT (MIST), Republic of Korea","award":["2020R1A2C2012721"],"award-info":[{"award-number":["2020R1A2C2012721"]}]},{"DOI":"10.13039\/501100003716","name":"Korea Basic Science Institute","doi-asserted-by":"crossref","award":["2019R1A6C1010003"],"award-info":[{"award-number":["2019R1A6C1010003"]}],"id":[{"id":"10.13039\/501100003716","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Korean government","award":["2120240615426"],"award-info":[{"award-number":["2120240615426"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Herpes simplex virus type 1 (HSV-1) causes recurrent mucocutaneous lesions, yet existing animal models incompletely recapitulate characteristic skin manifestations. Here, we established a mouse model of cutaneous HSV-1 infection by combining epithelial barrier disruption with localized viral inoculation. Superficial scarification of the ear followed by HSV-1 exposure resulted in consistent lesion formation. Administration of hydrocortisone further increased the incidence of lesions, highlighting the critical roles of epithelial integrity and host immune regulation in the pathogenesis of HSV-1 infection. Using this model, we evaluated the antiviral efficacy of Acyclovir. Treatment significantly reduced lesion severity, lesion size, and viral gene expression, indicating partial suppression of viral replication. Notably, acyclovir treatment was associated with increased expression of T-bet and Foxp3 in lymphoid tissues, suggesting modulation of both effector and regulatory immune responses. Collectively, this model successfully reproduces localized HSV-1 skin lesions, serving as a useful platform for investigating viral pathogenesis and evaluating antiviral therapies. However, further studies, including direct viral quantification and histopathological analysis, are required.<\/jats:p>","DOI":"10.3390\/pathogens15080787","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T13:53:26Z","timestamp":1784901206000},"page":"787","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Establishment of an HSV-1 Mouse Model with Cutaneous Lesions"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0349-9815","authenticated-orcid":false,"given":"Hye-Myung","family":"Ryu","sequence":"first","affiliation":[{"name":"Department of Microbiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5754-1324","authenticated-orcid":false,"given":"Bushra","family":"Riaz","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 16499, Republic of Korea"},{"name":"BK21 R&E Initiative for Advanced Precision Medicine, Graduate School of Ajou University, Suwon 16499, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seonghyang","family":"Sohn","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea"},{"name":"Department of Biomedical Sciences, Ajou University School of Medicine, Suwon 16499, Republic of Korea"},{"name":"BK21 R&E Initiative for Advanced Precision Medicine, Graduate School of Ajou University, Suwon 16499, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2670","DOI":"10.1080\/21505594.2021.1982373","article-title":"Pathogenesis and virulence of herpes simplex virus","volume":"12","author":"Zhu","year":"2021","journal-title":"Virulence"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e2491","DOI":"10.1002\/rmv.2491","article-title":"Immunopathology of herpes simplex virus-associated neuroinflammation: Unveiling the mysteries","volume":"34","author":"Hussain","year":"2024","journal-title":"Rev. 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