{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T17:10:02Z","timestamp":1785172202114,"version":"3.55.0"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: Leishmaniasis remains a major public health problem in Peru, and the Apur\u00edmac, Ene and Mantaro River Valley (VRAEM) is an endemic area where, despite its epidemiological relevance, information on incidence and circulating Leishmania species remains limited. Methods: We estimated district-level cumulative incidence of leishmaniasis in the 60 districts of the VRAEM during 2015\u20132024, using national surveillance data and census population figures. In addition, molecular species typing was performed on 112 Giemsa-stained tissue smears obtained in health facilities located in Ayacucho districts belonging to the VRAEM region. Results: A total of 3589 cases were reported, yielding an estimated annual incidence rate of 80.01 cases per 100,000 inhabitants, which is 4.3-fold higher than the national average. Marked spatial heterogeneity was observed, with districts such as Pangoa, R\u00edo Tambo, Llochegua, and Pichari reporting estimated annual incidence rates above 130 cases per 100,000 inhabitants. Furthermore, of 55 samples processed by High-Resolution Melting Analysis, Leishmania braziliensis was found in 54.6% of samples, followed by Leishmania guyanensis in 21.8%. Conclusions: The VRAEM constitutes a high-transmission endemic focus of tegumentary leishmaniasis with marked inter-district heterogeneity and circulation of multiple Leishmania species, with Leishmania braziliensis as the predominant species. These findings support the need for targeted surveillance and species-informed clinical management strategies.<\/jats:p>","DOI":"10.3390\/pathogens15080792","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T16:11:56Z","timestamp":1785168716000},"page":"792","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leishmaniasis Incidence and Leishmania Species Distribution in the Apur\u00edmac, Ene and Mantaro River Valley, a High-Transmission Endemic Area of Peru"],"prefix":"10.3390","volume":"15","author":[{"given":"Jime","family":"Rivera-Villar","sequence":"first","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas, Universidad Nacional San Crist\u00f3bal de Huamanga, Ayacucho 05001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1303-3744","authenticated-orcid":false,"given":"Nyshon","family":"Rojas-Palomino","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima 150108, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3755-5988","authenticated-orcid":false,"given":"Jos\u00e9","family":"Alarc\u00f3n-Guerrero","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas, Universidad Nacional San Crist\u00f3bal de Huamanga, Ayacucho 05001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"C\u00e1rdenas-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas, Universidad Nacional San Crist\u00f3bal de Huamanga, Ayacucho 05001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3610-1976","authenticated-orcid":false,"given":"Rilder","family":"Gastel\u00fa-Quispe","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas, Universidad Nacional San Crist\u00f3bal de Huamanga, Ayacucho 05001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aide","family":"Sandoval-Juarez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Salud P\u00fablica, Instituto Nacional de Salud, Lima 150108, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sa\u00fal","family":"Chuch\u00f3n-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas, Universidad Nacional San Crist\u00f3bal de Huamanga, Ayacucho 05001, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"ref_1","unstructured":"Organizaci\u00f3n Mundial de la Salud, and Organizaci\u00f3n Panamericana de la Salud (2023). Leishmaniasis: Informe Epidemiol\u00f3gico de las Am\u00e9ricas Num. 12, OPS."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/S1473-3099(16)00003-7","article-title":"Global burden of cutaneous leishmaniasis: A cross-sectional analysis from the Global Burden of Disease Study 2013","volume":"16","author":"Karimkhani","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Gabriel, \u00c1., Val\u00e9rio-Bolas, A., Palma-Marques, J., Mourata-Gon\u00e7alves, P., Ruas, P., Dias-Guerreiro, T., and Santos-Gomes, G. (2019). Cutaneous Leishmaniasis: The Complexity of Host\u2019s Effective Immune Response against a Polymorphic Parasitic Disease. J. Immunol. Res., 2019.","DOI":"10.1155\/2019\/2603730"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"607","DOI":"10.4269\/ajtmh.2004.70.607","article-title":"Epidemiology of Andean cutaneous leishmaniasis: Incrimination of Lutzomyia ayacuchensis (Diptera: Psychodidae) as a vector of Leishmania in geographically isolated, upland valleys of Peru","volume":"70","author":"Villaseca","year":"2004","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"De los Santos, M.B., Ram\u00edrez, I.M., Rodr\u00edguez, J.E., Beerli, P., and Valdivia, H.O. (2022). Genetic diversity and population structure of Leishmania (Viannia) braziliensis in the Peruvian jungle. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010374"},{"key":"ref_6","unstructured":"Centro Nacional de Epidemiologia Prevencion y Control de Enfermedades, and Ministerio de Salud (2024). Sala de Situaci\u00f3n de Salud. Semana Epidemiol\u00f3gica No. 52."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1590\/abd1806-4841.20142226","article-title":"American tegumentary leishmaniasis: Correlations among immunological, histopathological and clinical parameters","volume":"89","author":"Martins","year":"2014","journal-title":"An. Bras. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"312","DOI":"10.4269\/ajtmh.1998.59.312","article-title":"Geographic distribution and clinical description of leishmaniasis cases in Peru","volume":"59","author":"Lucas","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"87","DOI":"10.17843\/rpmesp.2020.371.4514","article-title":"Identificaci\u00f3n de especies de Leishmania en pacientes derivados al Instituto Nacional de Salud del Per\u00fa","volume":"37","year":"2020","journal-title":"Rev. Peru. Med. Exp. Salud P\u00fablica"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/j.ijpara.2008.08.004","article-title":"Typing of four genetic loci discriminates among closely related species of New World Leishmania","volume":"39","author":"Tsukayama","year":"2009","journal-title":"Int. J. Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zorrilla, V.O., Lozano, M.E., Espada, L.J., Kosoy, M., McKee, C., Valdivia, H.O., Arevalo, H., Troyes, M., Stoops, C.A., and Fisher, M.L. (2021). Comparison of sand fly trapping approaches for vector surveillance of Leishmania and Bartonella species in ecologically distinct, endemic regions of Peru. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009517"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3661","DOI":"10.1128\/JCM.00498-10","article-title":"Use of FTA cards for direct sampling of patients\u2019 lesions in the ecological study of cutaneous leishmaniasis","volume":"48","author":"Kato","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_13","first-page":"94","article-title":"Uso de anfotericina B liposomal en Leishmaniasis cutanea diseminada causada por Leishmania braziliensis en un paciente pedi\u00e1tico con s\u00edndrome de down","volume":"40","year":"2023","journal-title":"Rev. Peru. Med. Exp. Salud P\u00fablica"},{"key":"ref_14","first-page":"595","article-title":"Leishmaniosis cutanea: Manifestaci\u00f3n cl\u00ednica inusual","volume":"31","author":"Falconi","year":"2014","journal-title":"Rev. Peru. Med. Exp. Salud P\u00fablica"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"260","DOI":"10.4269\/ajtmh.1990.43.260","article-title":"Diffuse cutaneous leishmaniasis acquired in Peru","volume":"43","author":"Franke","year":"1990","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.abd.2021.03.003","article-title":"Successful treatment of diffuse cutaneous leishmaniasis caused by Leishmania amazonensis","volume":"96","author":"Sampaio","year":"2021","journal-title":"An. Bras. Dermatol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Scorza, B.M., Carvalho, E.M., and Wilson, M.E. (2017). Cutaneous manifestations of human and murine leishmaniasis. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18061296"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1159\/000441120","article-title":"Disseminated Cutaneous Leishmaniasis in Colombia: Report of 27 Cases","volume":"7","author":"Robledo","year":"2015","journal-title":"Case Rep. Dermatol."},{"key":"ref_19","unstructured":"Mendoza, W., and Leyva, J. (2017). La econom\u00eda del VRAEM. Diagn\u00f3stico y Opciones de Pol\u00edtica, Nova Print S.A.C.. [1st ed.]."},{"key":"ref_20","first-page":"326","article-title":"Post-senderismo, meta-seguridad y meta-violencia peruana en el caso VRAEM","volume":"9","year":"2020","journal-title":"Araucaria"},{"key":"ref_21","first-page":"1","article-title":"An\u00e1lisis de los determinantes sociales de la salud en el VRAEM","volume":"17","year":"2013","journal-title":"Rev. Peru. Epidemiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3768","DOI":"10.1128\/JCM.38.10.3768-3773.2000","article-title":"Diagnosis of cutaneous leishmaniasis in Colombia: The sampling site within lesions influences the sensitivity of parasitologic diagnosis","volume":"38","author":"Ramirez","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1128\/JCM.02555-06","article-title":"Detection and identification of Leishmania species from clinical specimens by using a real-time PCR assay and sequencing of the cytochrome B gene","volume":"45","author":"Foulet","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0001-706X(01)00140-1","article-title":"Sensitivity of the polymerase chain reaction for the diagnosis of cutaneous leishmaniasis due to Leishmania (Viannia) guyanensis","volume":"79","author":"Romero","year":"2001","journal-title":"Acta Trop."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Yupari-Azabache, I.L., D\u00edaz-Ortega, J.L., Bardales-Aguirre, L.B., Barros-Sevillano, S., and Paredes-D\u00edaz, S.E. (2023). Cluster Analysis of Factors Associated with Leishmaniasis in Peru. Trop. Med. Infect. Dis., 8.","DOI":"10.3390\/tropicalmed8110484"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1007\/s12639-023-01600-y","article-title":"Leishmaniases in a tropical area of Brazil: A spatio-temporal analysis","volume":"47","author":"Filho","year":"2023","journal-title":"J. Parasit. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1007\/s10393-014-0941-z","article-title":"Anthropogenic Land Use Change and Infectious Diseases: A Review of the Evidence","volume":"11","author":"Gottdenker","year":"2014","journal-title":"Ecohealth"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s41182-021-00332-0","article-title":"Risk factors for cutaneous leishmaniasis in a high-altitude forest region of Peru","volume":"49","author":"Lana","year":"2021","journal-title":"Trop. Med. Health"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1590\/15174522-88054","article-title":"Los Estados en los m\u00e1rgenes: Soberan\u00eda y gubernamentalidad en el principal valle cocalero peruano","volume":"21","author":"Castillo","year":"2019","journal-title":"Sociologias"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"23","DOI":"10.31389\/jied.7","article-title":"The Effect of Illicit Economies in the Margins of the State\u2014The VRAEM","volume":"1","author":"Heuser","year":"2019","journal-title":"J. Illicit. Econ. Dev."},{"key":"ref_31","unstructured":"Comisi\u00f3n Nacional para el Desarrollo y Vida sin Drogas (DEVIDA) (2025). Monitoreo de Cultivos de Coca 2024, DEVIDA."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Claborn, D.M. (2014). Conflict Leishmaniasis. Leishmaniasis\u2014Trends in Epidemiology, Diagnosis and Treatment, InTech.","DOI":"10.5772\/57067"},{"key":"ref_33","unstructured":"Ministerio de Salud (2025). Sala de Situaci\u00f3n de Salud. Semana Epidemiol\u00f3gica No. 52-2025."},{"key":"ref_34","first-page":"38","article-title":"Miner\u00eda informal e ilegal y contaminaci\u00f3n con mercurio en Madre de Dios: Un problema de salud p\u00fablica","volume":"29","author":"Plenge","year":"2012","journal-title":"Acta Med. Peru."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"De los Santos, M.B., Loyola, S., Perez-Velez, E.S., Santos, R.D.P., Ram\u00edrez, I.M., and Valdivia, H.O. (2024). Sampling is decisive to determination of Leishmania (Viannia) species. PLoS Negl. Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0012113"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kato, H., C\u00e1ceres, A.G., Seki, C., Garc\u00eda, C.R.S., Mauricci, C.H., Mart\u00ednez, S.C.C., Paico, D.M., Muniz, J.L.C., Rivera, L.D.T., and Briones, Z.I.V. (2019). Further insight into the geographic distribution of Leishmania species in Peru by cytochrome b and mannose phosphate isomerase gene analyses. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007496"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.actatropica.2016.02.024","article-title":"Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP","volume":"158","author":"Koarashi","year":"2016","journal-title":"Acta Trop."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/8\/792\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T16:23:54Z","timestamp":1785169434000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/8\/792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":37,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["pathogens15080792"],"URL":"https:\/\/doi.org\/10.3390\/pathogens15080792","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]}}}