{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:13:02Z","timestamp":1785402782549,"version":"3.56.0"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006742","name":"CESPU\u2014Cooperativa de Ensino Superior Polit\u00e9cnico e Universit\u00e1rio","doi-asserted-by":"publisher","award":["POUCAMRISK_GI2-CESPU_2023"],"award-info":[{"award-number":["POUCAMRISK_GI2-CESPU_2023"]}],"id":[{"id":"10.13039\/501100006742","id-type":"DOI","asserted-by":"publisher"}]},{"award":["POUCAMRISK_GI2-CESPU_2023"],"award-info":[{"award-number":["POUCAMRISK_GI2-CESPU_2023"]}],"id":[{"id":"https:\/\/ror.org\/00w7bj245","id-type":"ROR","asserted-by":"publisher"}]},{"name":"FCT\/MCTES","award":["UIDB\/50006\/2020 DOI 10.54499\/UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020 DOI 10.54499\/UIDB\/50006\/2020"]}]},{"name":"FCT\/MCTES","award":["UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDP\/50006\/2020"]}]},{"name":"FCT\/MCTES","award":["UIDB\/05937\/2020"],"award-info":[{"award-number":["UIDB\/05937\/2020"]}]},{"name":"FCT\/MCTES","award":["UIDP\/05937\/2020"],"award-info":[{"award-number":["UIDP\/05937\/2020"]}]},{"name":"FCT\/MCTES","award":["UID\/50027"],"award-info":[{"award-number":["UID\/50027"]}]},{"name":"FCT\/MCTES","award":["UIDP\/04378\/2020"],"award-info":[{"award-number":["UIDP\/04378\/2020"]}]},{"name":"FCT\/MCTES","award":["UIDB\/04378\/2020"],"award-info":[{"award-number":["UIDB\/04378\/2020"]}]},{"name":"FCT\/MCTES","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Campylobacter jejuni and C. coli are leading causes of bacterial gastroenteritis worldwide, commonly linked to undercooked poultry. Within a One Health perspective, understanding their dissemination from farm-to-slaughterhouse is essential. This study aimed to detect Campylobacter spp. in intensively reared broiler chickens at critical points along the production chain and to assess antimicrobial resistance genes. Two production cycles, November to December 2024 and July to August 2025, were monitored weekly across three Portuguese poultry farms and a shared slaughterhouse. In total, 2164 samples were collected, including farm, transport, and slaughterhouse samples. Detection followed ISO 10272-1:2017 and 10272-2:2017 standards. Campylobacter prevalence peaked during weeks 3 and 4 on farms (9.4% cloacal, 23.5% litter). Transport crates showed contamination before\/after loading (15.0%). At the slaughterhouse, contamination was low (8.3%) in process waters but high in neck skin samples (76.7%), with 15.2% exceeding the 1000 CFU\/g limit (EU Regulation 2017\/1495). Among 56 confirmed isolates, C. jejuni was most prevalent (46.4%), followed by C. coli (32.1%). The tetO gene was detected in most isolates (69.4%), while blaOXA-61 was less frequent (30.6%). Findings highlight the need for integrated control strategies, including improved biosecurity, crate sanitation, optimized processing procedures, systematic monitoring, and responsible antimicrobial use to enhance food safety and public health.<\/jats:p>","DOI":"10.3390\/pathogens15080803","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:09:42Z","timestamp":1785398982000},"page":"803","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dynamics of Campylobacter spp. Contamination in Broilers from Farm-to-Slaughter Chain"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9640-9377","authenticated-orcid":false,"given":"Carla","family":"Miranda","sequence":"first","affiliation":[{"name":"University Institute of Health Sciences (IUCS), Polytechnic and University Higher Education Cooperative (CESPU), Avenida Central de Gandra, 1317, 4585-116 Gandra, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), Avenida Central de Gandra 1317, 4585-116 Paredes, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, University Institute of Health Sciences CESPU, Rua Central de Gandra, 1317, 4585-116 Gandra, Portugal"},{"name":"LAQV-REQUIMTE\u2014Associated Laboratory for Green Chemistry, University NOVA of Lisbon, 1099-085 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Sousa","sequence":"additional","affiliation":[{"name":"University Institute of Health Sciences (IUCS), Polytechnic and University Higher Education Cooperative (CESPU), Avenida Central de Gandra, 1317, 4585-116 Gandra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6734-9863","authenticated-orcid":false,"given":"Ana","family":"Cardoso","sequence":"additional","affiliation":[{"name":"University Institute of Health Sciences (IUCS), Polytechnic and University Higher Education Cooperative (CESPU), Avenida Central de Gandra, 1317, 4585-116 Gandra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio","family":"Afonso","sequence":"additional","affiliation":[{"name":"University Institute of Health Sciences (IUCS), Polytechnic and University Higher Education Cooperative (CESPU), Avenida Central de Gandra, 1317, 4585-116 Gandra, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0622-7348","authenticated-orcid":false,"given":"Mariana Caipira","family":"Lei","sequence":"additional","affiliation":[{"name":"University Institute of Health Sciences (IUCS), Polytechnic and University Higher Education Cooperative (CESPU), Avenida Central de Gandra, 1317, 4585-116 Gandra, Portugal"},{"name":"Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1405-2277","authenticated-orcid":false,"given":"Nuno V.","family":"Brito","sequence":"additional","affiliation":[{"name":"University Institute of Health Sciences (IUCS), Polytechnic and University Higher Education Cooperative (CESPU), Avenida Central de Gandra, 1317, 4585-116 Gandra, Portugal"},{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, University Institute of Health Sciences (1H-TOXRUN, IUCS-CESPU), Avenida Central de Gandra 1317, 4585-116 Paredes, Portugal"},{"name":"Associate Laboratory i4HB\u2014Institute for Health and Bioeconomy, University Institute of Health Sciences CESPU, Rua Central de Gandra, 1317, 4585-116 Gandra, Portugal"},{"name":"CISAS\u2014Center for Research and Development in Agrifood Systems and Sustainability, Polytechnic Institute of Viana do Castelo, Rua Escola Industrial e Comercial de Nun\u2019\u00c1lvares, 4900-347 Viana do Castelo, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1128\/CMR.00006-15","article-title":"Global epidemiology of Campylobacter infection","volume":"28","author":"Kaakoush","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","unstructured":"EFSA (European Food Safety Authority) (2020). Update and review of control options for Campylobacter in broilers at primary production. EFSA J., 18, e06090."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"EFSA (European Food Safety Authority) (2024). The European Union One Health 2023 Zoonoses Report. EFSA J., 22, e9106.","DOI":"10.2903\/j.efsa.2024.9106"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Silva, J., Leite, D., Fernandes, M., Mena, C., Gibbs, P.A., and Teixeira, P. (2011). Campylobacter spp. as a foodborne pathogen: A review. Front. Microbiol., 2.","DOI":"10.3389\/fmicb.2011.00200"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e145","DOI":"10.1002\/puh2.145","article-title":"Probability of sequelae following Campylobacter spp. infections: Update of systematic reviews and meta-analyses","volume":"2","author":"Schorling","year":"2023","journal-title":"Public Health Chall."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1079\/AHRR200244","article-title":"Campylobacter colonization in poultry: Sources of infection and modes of transmission","volume":"3","author":"Sahin","year":"2002","journal-title":"Anim. Health Res. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.1017\/S0950268812000040","article-title":"The prevalence of Campylobacter spp. in broiler flocks and on broiler carcases, and the risks associated with highly contaminated carcases","volume":"140","author":"Powell","year":"2012","journal-title":"Epidemiol. Infect."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Biological Hazards (2011). Scientific opinion on Campylobacter in broiler meat production: Control options and performance objectives and\/or targets at different stages of the food chain. EFSA J., 9, 2105.","DOI":"10.2903\/j.efsa.2011.2105"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1086\/597417","article-title":"Attribution of Campylobacter infections in northeast Scotland to specific sources by use of multilocus sequence typing","volume":"199","author":"Strachan","year":"2009","journal-title":"J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.ijfoodmicro.2005.12.007","article-title":"The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter","volume":"108","author":"Rosenquist","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"ref_11","unstructured":"European Commission (2017). Commission Regulation (EU) 2017\/1495 of 23 August 2017 amending Regulation (EC) No 2073\/2005 as regards Campylobacter in broiler carcasses. Off. J. Eur. Union, Available online: https:\/\/eur-lex.europa.eu\/eli\/reg\/2017\/1495\/oj."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"189","DOI":"10.2217\/17460913.4.2.189","article-title":"Antibiotic resistance in Campylobacter: Emergence, transmission and persistence","volume":"4","author":"Luangtongkum","year":"2009","journal-title":"Future Microbiol."},{"key":"ref_13","unstructured":"European Medicines Agency (EMA) (2025). European Sales and Use of Antimicrobials for Veterinary Medicine (ESUAvet)\u2014Annual Surveillance Report for 2023, EMA\/CVMP\/ESUAVET\/80289\/2025."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"de Vries, S.P.W., Gupta, S., Baig, A., Wright, E., Wedley, A., Jensen, A.N., Anacarso, I., Humphrey, T.J., Williams, N.J., and de Cecco, M. (2018). Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0194481"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"908","DOI":"10.5455\/javar.2025.l952","article-title":"Phenotypic characterization and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli in broiler chickens and slaughterhouse workers in Algeria","volume":"12","author":"Messaoudene","year":"2025","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Tedersoo, T., Roasto, M., M\u00e4esaar, M., H\u00e4kkinen, L., Kisand, V., Ivanova, M., Valli, M.H., and Merem\u00e4e, K. (2022). Antibiotic resistance in Campylobacter spp. isolated from broiler chicken meat and human patients in Estonia. Microorganisms, 10.","DOI":"10.3390\/microorganisms10051067"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"EFSA (2019). The European Union One Health 2018 Zoonoses Report. EFSA J., 17, e05926.","DOI":"10.2903\/j.efsa.2019.5926"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2436060","DOI":"10.1080\/21505594.2024.2436060","article-title":"Pathogenicity and virulence of Campylobacter jejuni: What do we really know?","volume":"15","author":"Omole","year":"2024","journal-title":"Virulence"},{"key":"ref_19","unstructured":"(2017). Microbiology of the Food Chain\u2014Horizontal Method for Detection and Enumeration of Campylobacter spp.\u2014Part 1: Detection Method (Standard No. ISO Standard No. 10272-1:2017)."},{"key":"ref_20","unstructured":"(2017). Microbiology of the Food Chain\u2014Horizontal Method for Detection and Enumeration of Campylobacter spp.\u2014Part 2: Colony-Count Technique (Standard No. ISO Standard No. 10272-2:2017)."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Soares, A.S., Miranda, C., Coelho, A.C., and Trindade, H. (2023). Occurrence of coliforms and Enterococcus species in drinking water samples obtained from selected dairy cattle farms in Portugal. Agriculture, 13.","DOI":"10.3390\/agriculture13040885"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1128\/AEM.64.1.363-365.1998","article-title":"Differentiation of Campylobacter isolates on the basis of sensitivity to boiling in water as measured by PCR-detectable DNA","volume":"64","author":"Mohran","year":"1998","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/j.1745-4581.1992.tb00074.x","article-title":"A rapid PCR method for direct detection of low numbers of Campylobacter jejuni","volume":"1","author":"Wang","year":"1992","journal-title":"J. Rapid Methods Autom. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/S0923-2508(97)85118-2","article-title":"Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals","volume":"147","author":"Linton","year":"1996","journal-title":"Res. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2568","DOI":"10.1128\/jcm.35.10.2568-2572.1997","article-title":"PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples","volume":"35","author":"Linton","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1093\/jac\/dki040","article-title":"Tetracycline resistance of Australian Campylobacter jejuni and Campylobacter coli isolates","volume":"55","author":"Pratt","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1111\/j.1365-2672.2012.05354.x","article-title":"Antimicrobial susceptibilities and resistance genes in Campylobacter strains isolated from poultry and pigs in Australia","volume":"113","author":"Obeng","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Yang, M., Song, X., Wu, Z., Liu, J., Zhang, H., and Li, J. (2025). Genomic analysis and antimicrobial resistance in human Campylobacter jejuni isolates. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1599555"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1038\/s41579-018-0037-9","article-title":"Campylobacter jejuni: Collective components promoting a successful enteric lifestyle","volume":"16","author":"Burnham","year":"2018","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Reichelt, B., Szott, V., Epping, L., Semmler, T., Merle, R., Roesler, U., and Friese, A. (2022). Transmission pathways of Campylobacter spp. at broiler farms and their environment in Brandenburg, Germany. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.982693"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8605","DOI":"10.1128\/AEM.01090-10","article-title":"Biosecurity-based interventions and strategies to reduce Campylobacter spp. on poultry farms","volume":"77","author":"Newell","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1128\/AEM.01878-09","article-title":"Biofilm formation by Campylobacter jejuni is increased under aerobic conditions","volume":"76","author":"Reuter","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"104328","DOI":"10.1016\/j.psj.2024.104328","article-title":"Mechanistic concepts involved in biofilm associated processes of Campylobacter jejuni: Persistence and inhibition in poultry environments","volume":"103","author":"Bundurus","year":"2024","journal-title":"Poult. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Thompson, S.A., Corcionivoschi, N., Tresse, O., and Gundogdu, O. (2025). Recent advances in Campylobacter research. Front. Microbiol., 16.","DOI":"10.3389\/fmicb.2025.1646828"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Perez-Arnedo, I., and Gonzalez-Fandos, E. (2019). Prevalence of Campylobacter spp. in poultry in three Spanish farms, a slaughterhouse and a further processing plant. Foods, 8.","DOI":"10.3390\/foods8030111"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Mwangi, A., Kunyanga, C., Ngala, S., Kaindi, D.M., Sogin, J.H., Gitau, G.K., and Ndiritu, A. (2026). Prevalence, genotypic characterization, and hygiene-related risk factors of Campylobacter in broiler carcasses and on-farm slaughter environments in peri-urban Kenya. Front. Sustain. Food Syst., 10.","DOI":"10.3389\/fsufs.2026.1786685"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bai, Y., Ma, J., Li, F., Yang, B., Ren, X., Wang, Y., Hu, Y., Dong, Y., Wang, W., and Zhang, J. (2024). Antimicrobial resistance and genomic characterization of Campylobacter jejuni and Campylobacter coli isolated from retail chickens in Beijing, China. Microorganisms, 12.","DOI":"10.3390\/microorganisms12081601"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/8\/803\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T08:30:59Z","timestamp":1785400259000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/8\/803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":37,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["pathogens15080803"],"URL":"https:\/\/doi.org\/10.3390\/pathogens15080803","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]}}}