{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:48:57Z","timestamp":1785775737830,"version":"3.56.0"},"reference-count":77,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: The spread of extended-spectrum \u03b2-lactamase-producing Klebsiella is an emerging public health concern presenting severe clinical impacts. This problem is particularly severe in developing countries where irrational use of antibiotics makes the treatment of such infections more challenging. Methods: In this cross-sectional study, we investigated antibiotic resistance among clinical Klebsiella pneumoniae isolates from 256 patient specimens from Al-Diwaniyah hospitals, using standard microbiological methods followed by PCR. Antimicrobial susceptibility testing identified resistance rates and proportions of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. Carbapenemase genes were detected by multiplex PCR and sequencing. Mutations were characterized, and their functional significance was predicted using in silico prediction tools. Results: Fifty Klebsiella pneumoniae isolates were recovered, exhibiting high resistance rates (42\u2013100%) to penicillins, cephalosporins, fluoroquinolones and carbapenems, with 62% classified as MDR and 38% as XDR. Carbapenemase genes were highly prevalent (blaOXA-48 56%, blaIMP 44%, blaVIM 30%, blaKPC 28%, blaNDM 26%), with 60% of isolates co-harbouring \u2265 two genes. Most mutations were predicted to be structurally tolerated, while active-site-proximal substitutions (H120L in NDM-52 and V120L in OXA-48) were predicted to affect enzyme activity. However, docking analysis suggested no significant alteration in carbapenem binding affinity. Conclusions: Our results highlight a very high prevalence of MDR\/XDR Klebsiella pneumoniae, associated with diverse carbapenemase genes and resistance-related polymorphisms that may indicate potential functional impacts. To the best of our knowledge, this is the first study in Iraq to combine carbapenemase gene mutation analysis with structural modelling and molecular docking. This study emphasizes the urgent need for effective antimicrobial resistance surveillance in Iraq.<\/jats:p>","DOI":"10.3390\/pathogens15080819","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:51:23Z","timestamp":1785772283000},"page":"819","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterization and In Silico Functional Insights into Carbapenem Resistance in Clinical Klebsiella pneumoniae Isolates from Al-Diwaniyah, Iraq"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1394-3329","authenticated-orcid":false,"given":"Nada Ahmed","family":"Fairooz","sequence":"first","affiliation":[{"name":"Diwaniyah Technical Institute, AL-Furat AL-Awsat Technical University, Najaf 54003, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7046-487X","authenticated-orcid":false,"given":"Amal","family":"Ben Hassena","sequence":"additional","affiliation":[{"name":"Laboratory of Electrochemistry and Environment, National School of Engineers of Sfax, University of Sfax, BP 1173, Sfax 3038, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baheega Abees","family":"Al Khalidi","sequence":"additional","affiliation":[{"name":"College of Nursing, University of Al-Qadisiyah, Al-Diwaniyah 58001, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6045-0183","authenticated-orcid":false,"given":"Erdi Can","family":"Aytar","sequence":"additional","affiliation":[{"name":"Department of Horticulture, Faculty of Agriculture, Usak University, U\u015fak 64200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Sami","family":"Aifa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mounira","family":"Hmani","sequence":"additional","affiliation":[{"name":"Molecular and Functional Genetics Laboratory, Faculty of Science of Sfax, University of Sfax, Sfax 3000, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1086\/675598","article-title":"Frequency of Klebsiella pneumoniae carbapenemase (KPC)-producing and non-KPC-producing Klebsiella species contamination of healthcare workers and the environment","volume":"35","author":"Rock","year":"2014","journal-title":"Infect. 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