{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T11:29:45Z","timestamp":1786361385562,"version":"build-2736575974"},"reference-count":40,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Comprehensive Research Project","award":["VF.24.30"],"award-info":[{"award-number":["VF.24.30"]}]},{"name":"Elaz\u0131\u011f Veterinary Control Institute, Ministry of Agriculture and Forestry of the Republic of T\u00fcrkiye"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Babesia aktasi is a recently discovered species that is highly prevalent in native goats in T\u00fcrkiye\u2019s Mediterranean region. Although it does not induce clinical disease in local breeds, it causes severe illness in Saanen goats, manifesting with high fever, anemia, hemoglobinuria, and jaundice. Imidocarb dipropionate (IMDP) has been reported to be therapeutically effective against Babesia species. This study aimed to evaluate the therapeutic efficacy of IMDP and its ability to eliminate the parasite in experimentally infected Saanen goats. Twelve goats were assigned to treatment and control groups (n = 5 per group) and infected using fresh blood from two splenectomized donors. All goats developed clinical babesiosis, with parasitemia ranging from 3.8% to 22. Semi-nested PCR specific to B. aktasi was performed for 30 days post-treatment. Following treatment with IMDP (1.2 mg\/kg), clinical signs resolved by the third and fourth days, and parasitemia became microscopically undetectable. However, one goat in the treatment group and four goats in the control group died shortly after infection. Hematological parameters (HCT, RBC, HB) decreased during infection but normalized in surviving animals. Notably, B. aktasi DNA remained detectable by PCR up to 30 days after treatment. These findings indicated that IMDP resolved clinical signs and eliminated microscopically detectable parasitemia; however, it may not completely eliminate the parasite at the molecular level in B. aktasi infections. The results of this study provide valuable information for optimizing therapeutic approaches and improving our understanding of treatment responses in new species B. aktasi.<\/jats:p>","DOI":"10.3390\/pathogens15080833","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:23:50Z","timestamp":1786357430000},"page":"833","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Treatment Efficacy of Imidocarb Dipropionate in Saanen Goats Experimentally Infected with Babesia aktasi"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4492-143X","authenticated-orcid":false,"given":"Mehmet Can","family":"Ulucesme","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elaz\u0131\u011f 23200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sezayi","family":"Ozubek","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elaz\u0131\u011f 23200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3188-8757","authenticated-orcid":false,"given":"Munir","family":"Aktas","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elaz\u0131\u011f 23200, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S247","DOI":"10.1017\/S0031182004005190","article-title":"Babesiosis of Cattle","volume":"129","author":"Bock","year":"2004","journal-title":"Parasitology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1788","DOI":"10.1016\/j.meegid.2012.07.004","article-title":"Babesia: A world emerging","volume":"12","author":"Schnittger","year":"2012","journal-title":"Infect. Genet. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1146\/annurev-ento-011118-111932","article-title":"Vectors of babesiosis","volume":"64","author":"Gray","year":"2019","journal-title":"Annu. Rev. Entomol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.ijpara.2018.11.007","article-title":"Human Babesiosis","volume":"49","author":"Krause","year":"2019","journal-title":"Int. J. Parasitol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/s11250-025-04450-3","article-title":"A Review of babesiosis caused by Babesia ovis in small ruminants","volume":"57","author":"Esmaeilnejad","year":"2025","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1007\/s00436-022-07424-8","article-title":"The Piroplasmida Babesia, Cytauxzoon, and Theileria in farm and companion animals: Species compilation, molecular phylogeny, and evolutionary Insights","volume":"121","author":"Schnittger","year":"2022","journal-title":"Parasitol. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1007\/s00436-011-2658-z","article-title":"Babesia ovis as the main causative agent of sheep babesiosis in Iran","volume":"110","author":"Eckert","year":"2012","journal-title":"Parasitol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.vetpar.2012.07.025","article-title":"Babesia ovis infections: Detailed clinical and laboratory observations in the pre-and post-treatment periods of 97 field cases","volume":"191","author":"Sevinc","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Firat, R., Ulucesme, M.C., Aktas, M., Ceylan, O., Sevinc, F., Bastos, R.G., Suarez, C.E., and Ozubek, S. (2024). ole of Rhipicephalus bursa larvae in transstadial transmission and endemicity of Babesia ovis in chronically infected sheep. Front. Cell. Infect. Microbiol., 14.","DOI":"10.3389\/fcimb.2024.1428719"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.vetpar.2007.03.032","article-title":"At least two genetically distinct large Babesia species infective to sheep and goats in China","volume":"147","author":"Liu","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.exppara.2009.05.001","article-title":"new ovine Babesia species transmitted by Hyalomma anatolicum anatolicum","volume":"122","author":"Guan","year":"2009","journal-title":"Exp. Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2703","DOI":"10.1128\/JCM.02266-09","article-title":"Babesia lengau sp. nov., a novel Babesia species in cheetah (Acinonyx jubatus, Schreber, 1775) populations in South Africa","volume":"48","author":"Bosman","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.meegid.2016.03.007","article-title":"Genetic diversity and molecular characterization of Babesia motasi-like in small ruminants and ixodid ticks from China","volume":"41","author":"Niu","year":"2016","journal-title":"Infect. Genet. Evol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Ozubek, S., Ulucesme, M.C., and Aktas, M. (2023). Discovery of a novel species infecting goats: Morphological and molecular characterization of Babesia aktasi n. sp.. Pathogens, 12.","DOI":"10.3390\/pathogens12010113"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetpar.2016.11.016","article-title":"Molecular evidence of a new Babesia sp. in goats","volume":"233","author":"Ozubek","year":"2017","journal-title":"Vet. Parasitol."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ulucesme, M.C., Ozubek, S., Karoglu, A., Turk, Z.I., Olmus, I., Irehan, B., and Aktas, M. (2023). Small ruminant piroplasmosis: High prevalence of Babesia aktasi n. sp. in goats in T\u00fcrkiye. Pathogens, 12.","DOI":"10.3390\/pathogens12040514"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Ozubek, S., Ulucesme, M.C., Bastos, R.G., Alzan, H.F., Laughery, J.M., Suarez, C.E., and Aktas, M. (2023). Experimental infection of non-immunosuppressed and immunosuppressed goats reveals differential pathogenesis of Babesia aktasi n. sp. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1277956"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Ulucesme, M.C., Ozubek, S., and Aktas, M. (2024). Experimental infection of purebred Saanen goats high pathogenicity and virulence of Babesia aktasi. PLoS Neglected Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0012705"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Ulucesme, M.C., Ozubek, S., and Aktas, M. (2024). Sheep displayed no clinical and parasitological signs upon experimental infection with Babesia aktasi. Vet. Sci., 11.","DOI":"10.3390\/vetsci11080359"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.2174\/092986712799828355","article-title":"Current advances in detection and treatment of babesiosis","volume":"19","author":"Mosqueda","year":"2012","journal-title":"Curr. Med. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"109013","DOI":"10.1016\/j.vetpar.2019.109013","article-title":"Assay methods for in vitro and in vivo anti-Babesia drug efficacy testing: Current progress, outlook, and challenges","volume":"279","author":"Rizk","year":"2020","journal-title":"Vet. Parasitol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/S0034-5288(18)32501-3","article-title":"Investigations on Babesia motasi isolated from wales","volume":"31","author":"Lewis","year":"1981","journal-title":"Res. Vet. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.vetpar.2006.01.048","article-title":"Chemotherapy against babesiosis","volume":"138","author":"Vial","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/S0034-5288(18)30565-4","article-title":"Efficacy, toxicity and metabolism of imidocarb dipropionate in the treatment of Babesia ovis infection in sheep","volume":"41","author":"McHardy","year":"1986","journal-title":"Res. Vet. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1128\/CMR.16.4.622-636.2003","article-title":"Babesia divergens, a bovine blood parasite of veterinary and zoonotic importance","volume":"16","author":"Zintl","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.vetpar.2007.07.014","article-title":"Therapeutic and prophylactic efficacy of imidocarb dipropionate on experimental Babesia ovis infection of lambs","volume":"149","author":"Sevinc","year":"2007","journal-title":"Vet. Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Sears, K., Knowles, D., Dinkel, K., Mshelia, P.W., Onzere, C., Silva, M., and Fry, L. (2020). Imidocarb dipropionate lacks efficacy against Theileria haneyi and fails to consistently clear Theileria equi in horses co-infected with T. haneyi. Pathogens, 9.","DOI":"10.20944\/preprints202011.0379.v1"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"102282","DOI":"10.1016\/j.ttbdis.2023.102282","article-title":"Babesia negevi infection in dogs and response to treatment","volume":"15","author":"Salant","year":"2024","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_29","unstructured":"Kuttler, K.L. (1988). World-Wide Impact of babesiosis. Babesiosis of Domestic Animals and Man, CRC Press."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vetpar.2018.03.001","article-title":"Antiprotozoal treatment of canine babesiosis","volume":"254","author":"Baneth","year":"2018","journal-title":"Vet. Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2817","DOI":"10.1007\/s00436-013-3452-x","article-title":"Molecular identification of Theileria and Babesia in sheep and goats in the Black Sea Region in Turkey","volume":"112","author":"Aydin","year":"2013","journal-title":"Parasitol. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1186\/s13071-021-04712-7","article-title":"Assessment of Babesia bovis 6cys A and 6cys B as components of transmission blocking vaccines for babesiosis","volume":"14","author":"Alzan","year":"2021","journal-title":"Parasites Vectors"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Bastos, R.G., Laughery, J.M., Ozubek, S., Alzan, H.F., Taus, N.S., Ueti, M.W., and Suarez, C.E. (2022). Identification of novel immune correlates of protection against acute bovine babesiosis by superinfecting cattle with in vitro culture attenuated and virulent Babesia bovis strains. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.1045608"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1038\/s41541-023-00741-8","article-title":"Vaccination of cattle with the Babesia bovis sexual-stage protein HAP2 abrogates parasite transmission by Rhipicephalus microplus ticks","volume":"8","author":"Silva","year":"2023","journal-title":"npj Vaccines"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Ulucesme, M.C., Ozubek, S., and Aktas, M. (2024). Incompetence of vector capacity of Rhipicephalus bursa to transmit Babesia aktasi following feeding on clinically infected goat with high level of parasitemia. Vet. Sci., 11.","DOI":"10.3390\/vetsci11070309"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Ulucesme, M.C., Ozubek, S., and Aktas, M. (2024). Development and evaluation of a semi-nested PCR method based on the 18S Ribosomal RNA gene for the detection of Babesia aktasi infections in goats. Vet. Sci., 11.","DOI":"10.3390\/vetsci11100466"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1136\/vr.129.17.383","article-title":"Ovine and caprine babesiosis in Iran: Treatment with Iimidocarb","volume":"129","year":"1991","journal-title":"Vet. Rec."},{"key":"ref_38","first-page":"31","article-title":"Investigation of hematological and biochemical parameters in small ruminants naturally infected with Babesia ovis","volume":"3","author":"Esmaeilnejad","year":"2012","journal-title":"Vet. Res. Forum"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1007\/s12639-019-01109-3","article-title":"Detection of incidence of Babesia spp. in sheep and goats by parasitological diagnostic techniques","volume":"43","author":"Kage","year":"2019","journal-title":"J. Parasit. Dis."},{"key":"ref_40","unstructured":"Latimer, K.S. (2011). Erythrocytes. Duncan and Prasse\u2019s Veterinary Laboratory Medicine: Clinical Pathology, Iowa State University Press."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/8\/833\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:48:08Z","timestamp":1786358888000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/15\/8\/833"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":40,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["pathogens15080833"],"URL":"https:\/\/doi.org\/10.3390\/pathogens15080833","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]}}}