{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:57:06Z","timestamp":1786978626016,"version":"build-2736575974"},"reference-count":52,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>ZIKV is an emerging mosquito-borne pathogen with the unique capability to cause severe congenital abnormalities. Mosquito-borne viruses are reliant on an optimal temperature within the mosquito host to spread to humans. Few studies explore how day\/night temperature variations impact virus replication within the mosquito. Most laboratory studies are conducted at constant temperatures, or with very minimal temperature oscillations. In consequence, ZIKV transmission models and risk maps utilize biological data derived from constant temperature studies. We sought to determine if the use of viral replication data obtained in more biologically relevant, fluctuating temperatures alters the geographic range for ZIKV transmission risk. We used a growing degree day (GDD)-based model to determine ZIKV transmission potential across the US. Replication curves were conducted at eight different average temperatures between 18\u00b0C and 32\u00b0C with daily fluctuations of either +\/\u22122.5\u00b0C or +\/\u22125\u00b0C from the baseline. We found that at lower temperatures (18\u201322\u00b0C), fluctuation was beneficial for virus replication, where it had less of an impact at more optimal temperatures (24\u201330\u00b0C). Our transmission potential map exhibited the highest proportion of infected mosquitoes in southeastern United States, with discrete pockets of elevated potential across interior regions, including the southern Great Plains and lower Mississippi Valley.<\/jats:p>","DOI":"10.3390\/pathogens15080839","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T14:45:06Z","timestamp":1786545906000},"page":"839","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of Day\/Night Cycle Temperature Regimes on Zika Virus Replication in Mosquito Cells and Implications for Transmission Potential"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-5929-1941","authenticated-orcid":false,"given":"Breanna R.","family":"Timani","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel N.","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine D.","family":"Shields","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mekala","family":"Sundaram","sequence":"additional","affiliation":[{"name":"Savannah River Ecology Laboratory, University of Georgia, Athens, GA 30602, USA"},{"name":"Department of Infectious Diseases and Center for Precision One Health, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4929-8085","authenticated-orcid":false,"given":"Melinda A.","family":"Brindley","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2022). 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