{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:21:45Z","timestamp":1787235705224,"version":"build-2736575974"},"reference-count":53,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI21CIII\/00034"],"award-info":[{"award-number":["PI21CIII\/00034"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"award":["PI21CIII\/00034"],"award-info":[{"award-number":["PI21CIII\/00034"]}],"id":[{"id":"https:\/\/ror.org\/00ca2c886","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004892","name":"Spanish Agency for International Development Cooperation","doi-asserted-by":"publisher","award":["2024\/SUN\/0000500012"],"award-info":[{"award-number":["2024\/SUN\/0000500012"]}],"id":[{"id":"10.13039\/501100004892","id-type":"DOI","asserted-by":"publisher"}]},{"award":["2024\/SUN\/0000500012"],"award-info":[{"award-number":["2024\/SUN\/0000500012"]}],"id":[{"id":"https:\/\/ror.org\/00r6akf90","id-type":"ROR","asserted-by":"publisher"}]},{"name":"CIBERINFEC, ISCIII","award":["INFEC24PI05"],"award-info":[{"award-number":["INFEC24PI05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Malaria diagnosis in Equatorial Guinea (EG) is based on conventional microscopy, sometimes combined with rapid diagnostic tests (RDTs). However, some laboratories, such as the Baney Research Laboratory (BRL) have implemented molecular technology for the diagnosis of infectious diseases. This study aimed to evaluate, for the first time in EG, a LAMP method for malaria diagnosis, comparing its performance with conventional microscopy performed in EG and with LAMP and PCR diagnostic methods performed at the National Centre of Tropical Medicine (NCTM), Spain. A total of 178 blood samples (including P. falciparum samples and malaria-negative controls) were evaluated using Dual-LAMP-Pspp and Nested-PCR-Pf in Equatorial Guinea. Dual-LAMP-Pspp performed at the BRL showed sensitivity and specificity values of 97.28% and 100%, respectively, for malaria diagnosis, with no differences between colorimetric and fluorescence-based result readings. Meanwhile, Nested-PCR-Pf showed a sensitivity of 87.07% and a specificity of 100%. Comparison of parasite densities determined by Dual-LAMP-Pspp and conventional microscopy showed a significant moderate negative correlation (correlation coefficient = \u22120.584). The comparison of Dual-LAMP-Pspp results between BRL and NCTM showed a kappa coefficient of 0.93, whereas the kappa coefficient of the Nested-PCR-Pf assay was 0.70. These findings indicate that Dual-LAMP-Pspp and Nested-PCR-Pf are suitable methods for malaria diagnosis in EG. However, the Dual-LAMP-Pspp assay was easy to perform and interpret and provided results in a shorter time than Nested-PCR-Pf.<\/jats:p>","DOI":"10.3390\/pathogens15080872","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T09:48:31Z","timestamp":1787219311000},"page":"872","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Loop-Mediated Isothermal Amplification (LAMP) Assessment for Malaria Diagnosis in Equatorial Guinea"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1132-2211","authenticated-orcid":false,"given":"Alexandra","family":"Mart\u00edn-Ram\u00edrez","sequence":"first","affiliation":[{"name":"Malaria and Emerging Parasites Laboratory, National Center for Microbiology, Institute of Health Carlos III, 28222 Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teodora","family":"Mikumu Alogo","sequence":"additional","affiliation":[{"name":"Baney Research Laboratory, Baney P.O. Box 338, Equatorial Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Palacios","sequence":"additional","affiliation":[{"name":"Baney Research Laboratory, Baney P.O. Box 338, Equatorial Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monserrat","family":"Kobe Elonga","sequence":"additional","affiliation":[{"name":"Baney Research Laboratory, Baney P.O. Box 338, Equatorial Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Mangue","sequence":"additional","affiliation":[{"name":"Baney Research Laboratory, Baney P.O. Box 338, Equatorial Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Molina-de la Fuente","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"Malariology Unit, Institute of Tropical Medicine of Antwerp, 2000 Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicenta","family":"Gonz\u00e1lez Mora","sequence":"additional","affiliation":[{"name":"Malaria and Emerging Parasites Laboratory, National Center for Microbiology, Institute of Health Carlos III, 28222 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Lanza-Su\u00e1rez","sequence":"additional","affiliation":[{"name":"Malaria and Emerging Parasites Laboratory, National Center for Microbiology, Institute of Health Carlos III, 28222 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Rodr\u00edguez-Galet","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"National Center of Tropical Medicine, Institute of Health Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilde","family":"Riloha Rivas","sequence":"additional","affiliation":[{"name":"National Malaria Programme, Ministry of Health and Social Welfare, Government of the Republic of Equatorial Guinea, Malabo, Equatorial Guinea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Policarpo","family":"Ncogo","sequence":"additional","affiliation":[{"name":"Spanish State Foundation, Health, Children and Social Welfare, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9468-3102","authenticated-orcid":false,"given":"Agust\u00edn","family":"Benito","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain"},{"name":"National Center of Tropical Medicine, Institute of Health Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1903-6711","authenticated-orcid":false,"given":"Jos\u00e9 Miguel","family":"Rubio","sequence":"additional","affiliation":[{"name":"Malaria and Emerging Parasites Laboratory, National Center for Microbiology, Institute of Health Carlos III, 28222 Madrid, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas, Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Nyakarungu","sequence":"additional","affiliation":[{"name":"Baney Research Laboratory, Baney P.O. 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